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Interaction of regular exercise and chronic nitroglycerin treatment on blood pressure and rat aortic antioxidants.
Biochim Biophys Acta. 2004 Jan 20; 1688(1):18-25.BB

Abstract

Many cardiac patients undergo exercise conditioning with or without medication. Therefore, we investigated the interaction of exercise training and chronic nitroglycerin treatment on blood pressure (BP), aortic nitric oxide (NO), oxidants and antioxidants in rats. Fisher 344 rats were divided into four groups and treated as follows: (1) sedentary control, (2) exercise training (ET) for 8 weeks, (3) nitroglycerin (15 mg/kg, s.c. for 8 weeks) and (4) ET+nitroglycerin. BP was monitored with tail-cuff method. The animals were sacrificed 24 h after the last treatments and thoracic aorta was isolated and analyzed. Exercise training on treadmill for 8 weeks significantly increased respiratory exchange ratio (RER), aortic NO levels, and endothelial nitric oxide synthase (eNOS) protein expression. Training significantly enhanced aortic glutathione (GSH), reduced to oxidized glutathione (GSH/GSSG) ratio, copper/zinc-superoxide dismutase (CuZn-SOD), Mn-SOD, catalase (CAT), glutathione peroxidase (GSH-Px) glutathione disulfide reductase (GR) activities and protein expressions. Training significantly depleted aortic malondialdehyde (MDA) and protein carbonyls without change in BP. Nitroglycerin administration for 8 weeks significantly increased aortic NO levels and eNOS protein expression. Nitroglycerin significantly enhanced aortic Mn-SOD, CAT, GR and glutathione-S-transferase (GST) activities and protein expressions with decreased MDA levels, protein carbonyls and BP. Interaction of training and nitroglycerin treatment significantly increased aortic NO levels, eNOS protein expression, GSH/GSSG ratio, antioxidant enzymes and normalized BP. The data suggest that the interaction of training and nitroglycerin maintained BP by up-regulating the aortic NO and antioxidants and reducing the oxidative stress in rats.

Authors+Show Affiliations

Department of Surgery, Southern Illinois University School of Medicine, Springfield, IL, USA. khusain@psm.edu

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14732477

Citation

Husain, Kazim. "Interaction of Regular Exercise and Chronic Nitroglycerin Treatment On Blood Pressure and Rat Aortic Antioxidants." Biochimica Et Biophysica Acta, vol. 1688, no. 1, 2004, pp. 18-25.
Husain K. Interaction of regular exercise and chronic nitroglycerin treatment on blood pressure and rat aortic antioxidants. Biochim Biophys Acta. 2004;1688(1):18-25.
Husain, K. (2004). Interaction of regular exercise and chronic nitroglycerin treatment on blood pressure and rat aortic antioxidants. Biochimica Et Biophysica Acta, 1688(1), 18-25.
Husain K. Interaction of Regular Exercise and Chronic Nitroglycerin Treatment On Blood Pressure and Rat Aortic Antioxidants. Biochim Biophys Acta. 2004 Jan 20;1688(1):18-25. PubMed PMID: 14732477.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interaction of regular exercise and chronic nitroglycerin treatment on blood pressure and rat aortic antioxidants. A1 - Husain,Kazim, PY - 2004/1/21/pubmed PY - 2004/4/21/medline PY - 2004/1/21/entrez SP - 18 EP - 25 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1688 IS - 1 N2 - Many cardiac patients undergo exercise conditioning with or without medication. Therefore, we investigated the interaction of exercise training and chronic nitroglycerin treatment on blood pressure (BP), aortic nitric oxide (NO), oxidants and antioxidants in rats. Fisher 344 rats were divided into four groups and treated as follows: (1) sedentary control, (2) exercise training (ET) for 8 weeks, (3) nitroglycerin (15 mg/kg, s.c. for 8 weeks) and (4) ET+nitroglycerin. BP was monitored with tail-cuff method. The animals were sacrificed 24 h after the last treatments and thoracic aorta was isolated and analyzed. Exercise training on treadmill for 8 weeks significantly increased respiratory exchange ratio (RER), aortic NO levels, and endothelial nitric oxide synthase (eNOS) protein expression. Training significantly enhanced aortic glutathione (GSH), reduced to oxidized glutathione (GSH/GSSG) ratio, copper/zinc-superoxide dismutase (CuZn-SOD), Mn-SOD, catalase (CAT), glutathione peroxidase (GSH-Px) glutathione disulfide reductase (GR) activities and protein expressions. Training significantly depleted aortic malondialdehyde (MDA) and protein carbonyls without change in BP. Nitroglycerin administration for 8 weeks significantly increased aortic NO levels and eNOS protein expression. Nitroglycerin significantly enhanced aortic Mn-SOD, CAT, GR and glutathione-S-transferase (GST) activities and protein expressions with decreased MDA levels, protein carbonyls and BP. Interaction of training and nitroglycerin treatment significantly increased aortic NO levels, eNOS protein expression, GSH/GSSG ratio, antioxidant enzymes and normalized BP. The data suggest that the interaction of training and nitroglycerin maintained BP by up-regulating the aortic NO and antioxidants and reducing the oxidative stress in rats. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/14732477/Interaction_of_regular_exercise_and_chronic_nitroglycerin_treatment_on_blood_pressure_and_rat_aortic_antioxidants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0925443903001662 DB - PRIME DP - Unbound Medicine ER -