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Local application of the cannabinoid receptor agonist, WIN 55,212-2, to spinal trigeminal nucleus caudalis differentially affects nociceptive and non-nociceptive neurons.
Pain. 2004 Feb; 107(3):267-275.PAIN

Abstract

Cannabinoid receptor agonists produce analgesia for pains of non-cranial origin. However, their effectiveness for craniofacial pains is currently unclear. In the present study, the cannabinoid CB1/CB2 receptor agonist, WIN 55,212-2 (WIN), was bath applied to the brainstem while activity of spinal trigeminal nucleus caudalis (Vc) neurons evoked by transcutaneous electrical stimulation was recorded in isoflurane anesthetized rats. Neurons were characterized using mechanical and electrical stimulation of the face, and were classified as either low-threshold mechanoreceptive (LTM) or wide dynamic range (WDR). LTM neurons responded to light brushing of the receptive field and received only Abeta primary afferent fiber input. WDR neurons showed a graded response to mechanical stimulation, responding maximally to noxious stimuli, and demonstrated both A- and C-fiber evoked activity. In addition, WDR neurons displayed longer latency, C-fiber mediated post-discharge (PDC) activity after repetitive stimulation. Local bath application of 2.0 mg/ml WIN significantly reduced PDC activity (3+/-1% control, P<0.01), C-fiber evoked activity (58+/-9% control, P<0.01), and Abeta evoked activity (57+/-10% control, P<0.01) in WDR neurons. In contrast, LTM Abeta-fiber evoked activity increased after local administration of WIN (204+/-52% control, P<0.01). SR141716A, a CB1 receptor antagonist, prevented the effects of WIN on WDR PDC and LTM Abeta evoked activity. These results indicate that cannabinoid receptor agonists may be effective agents for craniofacial pain. Furthermore, the particular sensitivity of PDC activity, a measure of neuronal hyperexcitability, to cannabinoid receptor agonists may be relevant to the treatment of persistent craniofacial pain.

Authors+Show Affiliations

Department of Neurology, University of California, San Francisco, CA 94143-0114, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14736589

Citation

Papanastassiou, Alex M., et al. "Local Application of the Cannabinoid Receptor Agonist, WIN 55,212-2, to Spinal Trigeminal Nucleus Caudalis Differentially Affects Nociceptive and Non-nociceptive Neurons." Pain, vol. 107, no. 3, 2004, pp. 267-275.
Papanastassiou AM, Fields HL, Meng ID. Local application of the cannabinoid receptor agonist, WIN 55,212-2, to spinal trigeminal nucleus caudalis differentially affects nociceptive and non-nociceptive neurons. Pain. 2004;107(3):267-275.
Papanastassiou, A. M., Fields, H. L., & Meng, I. D. (2004). Local application of the cannabinoid receptor agonist, WIN 55,212-2, to spinal trigeminal nucleus caudalis differentially affects nociceptive and non-nociceptive neurons. Pain, 107(3), 267-275. https://doi.org/10.1016/j.pain.2003.11.009
Papanastassiou AM, Fields HL, Meng ID. Local Application of the Cannabinoid Receptor Agonist, WIN 55,212-2, to Spinal Trigeminal Nucleus Caudalis Differentially Affects Nociceptive and Non-nociceptive Neurons. Pain. 2004;107(3):267-275. PubMed PMID: 14736589.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Local application of the cannabinoid receptor agonist, WIN 55,212-2, to spinal trigeminal nucleus caudalis differentially affects nociceptive and non-nociceptive neurons. AU - Papanastassiou,Alex M, AU - Fields,Howard L, AU - Meng,Ian D, PY - 2004/1/23/pubmed PY - 2004/4/13/medline PY - 2004/1/23/entrez SP - 267 EP - 275 JF - Pain JO - Pain VL - 107 IS - 3 N2 - Cannabinoid receptor agonists produce analgesia for pains of non-cranial origin. However, their effectiveness for craniofacial pains is currently unclear. In the present study, the cannabinoid CB1/CB2 receptor agonist, WIN 55,212-2 (WIN), was bath applied to the brainstem while activity of spinal trigeminal nucleus caudalis (Vc) neurons evoked by transcutaneous electrical stimulation was recorded in isoflurane anesthetized rats. Neurons were characterized using mechanical and electrical stimulation of the face, and were classified as either low-threshold mechanoreceptive (LTM) or wide dynamic range (WDR). LTM neurons responded to light brushing of the receptive field and received only Abeta primary afferent fiber input. WDR neurons showed a graded response to mechanical stimulation, responding maximally to noxious stimuli, and demonstrated both A- and C-fiber evoked activity. In addition, WDR neurons displayed longer latency, C-fiber mediated post-discharge (PDC) activity after repetitive stimulation. Local bath application of 2.0 mg/ml WIN significantly reduced PDC activity (3+/-1% control, P<0.01), C-fiber evoked activity (58+/-9% control, P<0.01), and Abeta evoked activity (57+/-10% control, P<0.01) in WDR neurons. In contrast, LTM Abeta-fiber evoked activity increased after local administration of WIN (204+/-52% control, P<0.01). SR141716A, a CB1 receptor antagonist, prevented the effects of WIN on WDR PDC and LTM Abeta evoked activity. These results indicate that cannabinoid receptor agonists may be effective agents for craniofacial pain. Furthermore, the particular sensitivity of PDC activity, a measure of neuronal hyperexcitability, to cannabinoid receptor agonists may be relevant to the treatment of persistent craniofacial pain. SN - 0304-3959 UR - https://www.unboundmedicine.com/medline/citation/14736589/Local_application_of_the_cannabinoid_receptor_agonist_WIN_55212_2_to_spinal_trigeminal_nucleus_caudalis_differentially_affects_nociceptive_and_non_nociceptive_neurons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/00006396-200402000-00010 DB - PRIME DP - Unbound Medicine ER -