Tags

Type your tag names separated by a space and hit enter

Improvement of dermatitis by iontophoretically delivered antisense oligonucleotides for interleukin-10 in NC/Nga mice.
Gene Ther. 2004 Feb; 11(3):317-24.GT

Abstract

IL-10 is overexpressed in skin lesions of atopic dermatitis (AD) patients and believed to be an important factor in the pathogenesis of the disease. Thus the regulation of IL-10 production is a potential solution for immunotherapeutic intervention in AD. We examined the topical delivery of an antisense oligonucleotide for mouse IL-10 (AS6) and the therapeutic effect on the skin lesions of NC/Nga mice, a human AD model. Using an iontophoresis system, about 30% of the applied dose of AS6 penetrated the skin and was distributed in the epidermis and upper dermis. Topically delivered AS6 decreased the levels of mRNA and protein of IL-10 in the lesions of NC/Nga mice, with no effect on IL-4 levels. The dorsal lesions of NC/Nga mice disappeared with repeated topical application of AS6. Topically delivered AS6 showed an inhibitory effect on the production of IL-10 in the skin lesions of NC/Nga mice and had a therapeutic effect on the established dermatitis.

Authors+Show Affiliations

School of Pharmacy, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14737092

Citation

Sakamoto, T, et al. "Improvement of Dermatitis By Iontophoretically Delivered Antisense Oligonucleotides for Interleukin-10 in NC/Nga Mice." Gene Therapy, vol. 11, no. 3, 2004, pp. 317-24.
Sakamoto T, Miyazaki E, Aramaki Y, et al. Improvement of dermatitis by iontophoretically delivered antisense oligonucleotides for interleukin-10 in NC/Nga mice. Gene Ther. 2004;11(3):317-24.
Sakamoto, T., Miyazaki, E., Aramaki, Y., Arima, H., Takahashi, M., Kato, Y., Koga, M., & Tsuchiya, S. (2004). Improvement of dermatitis by iontophoretically delivered antisense oligonucleotides for interleukin-10 in NC/Nga mice. Gene Therapy, 11(3), 317-24.
Sakamoto T, et al. Improvement of Dermatitis By Iontophoretically Delivered Antisense Oligonucleotides for Interleukin-10 in NC/Nga Mice. Gene Ther. 2004;11(3):317-24. PubMed PMID: 14737092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improvement of dermatitis by iontophoretically delivered antisense oligonucleotides for interleukin-10 in NC/Nga mice. AU - Sakamoto,T, AU - Miyazaki,E, AU - Aramaki,Y, AU - Arima,H, AU - Takahashi,M, AU - Kato,Y, AU - Koga,M, AU - Tsuchiya,S, PY - 2004/1/23/pubmed PY - 2004/3/17/medline PY - 2004/1/23/entrez SP - 317 EP - 24 JF - Gene therapy JO - Gene Ther VL - 11 IS - 3 N2 - IL-10 is overexpressed in skin lesions of atopic dermatitis (AD) patients and believed to be an important factor in the pathogenesis of the disease. Thus the regulation of IL-10 production is a potential solution for immunotherapeutic intervention in AD. We examined the topical delivery of an antisense oligonucleotide for mouse IL-10 (AS6) and the therapeutic effect on the skin lesions of NC/Nga mice, a human AD model. Using an iontophoresis system, about 30% of the applied dose of AS6 penetrated the skin and was distributed in the epidermis and upper dermis. Topically delivered AS6 decreased the levels of mRNA and protein of IL-10 in the lesions of NC/Nga mice, with no effect on IL-4 levels. The dorsal lesions of NC/Nga mice disappeared with repeated topical application of AS6. Topically delivered AS6 showed an inhibitory effect on the production of IL-10 in the skin lesions of NC/Nga mice and had a therapeutic effect on the established dermatitis. SN - 0969-7128 UR - https://www.unboundmedicine.com/medline/citation/14737092/Improvement_of_dermatitis_by_iontophoretically_delivered_antisense_oligonucleotides_for_interleukin_10_in_NC/Nga_mice_ L2 - https://doi.org/10.1038/sj.gt.3302171 DB - PRIME DP - Unbound Medicine ER -