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Immunoassay using probe-labelling immunogold nanoparticles with silver staining enhancement via surface-enhanced Raman scattering.
Analyst. 2004 Jan; 129(1):63-8.A

Abstract

This paper reports a novel immunoassay based on surface-enhanced Raman scattering (SERS) and immunogold labelling with silver staining enhancement. Immunoreactions between immunogold colloids modified by a Raman-active probe molecule (e.g., 4-mercaptobenzoic acid) and antigens, which were captured by antibody-assembled chips such as silicon or quartz, were detected via SERS signals of Raman-active probe molecule. All the self-assembled steps were subjected to the measurements of ultraviolet-visible (UV-vis) spectra to monitor the formation of a sandwich structure onto a substrate. The immunoassay was performed by a sandwich structure consisting of three layers. The first layer was composed of immobilized antibody molecules of mouse polyclonal antibody against Hepatitis B virus surface antigen (PAb) on a silicon or quartz substrate. The second layer was the complementary Hepatitis B virus surface antigen (Antigen) molecules captured by PAb on the substrate. The third layer was composed of the probe-labelling immunogold nanoparticles, which were modified by mouse monoclonal antibody against Hepatitis B virus surface antigen (MAb) and 4-mercaptobenzoic acid (MBA) as the Raman-active probe on the surface of gold colloids. After silver staining enhancement, the antigen is identified by a SERS spectrum of MBA. A working curve of the intensity of a SERS signal at 1585 cm(-1) due to the [small nu](8a) aromatic ring vibration of MBA versus the concentration of analyte (Antigen) was obtained and the non-optimized detection limit for the Hepatitis B virus surface antigen was found to be as low as 0.5 [micro sign]g mL(-1).

Authors+Show Affiliations

Key Laboratory for Supramolecular Structure and Material of Ministry of Education, Jilin University, Changchun 130021, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14737585

Citation

Xu, Shuping, et al. "Immunoassay Using Probe-labelling Immunogold Nanoparticles With Silver Staining Enhancement Via Surface-enhanced Raman Scattering." The Analyst, vol. 129, no. 1, 2004, pp. 63-8.
Xu S, Ji X, Xu W, et al. Immunoassay using probe-labelling immunogold nanoparticles with silver staining enhancement via surface-enhanced Raman scattering. Analyst. 2004;129(1):63-8.
Xu, S., Ji, X., Xu, W., Li, X., Wang, L., Bai, Y., Zhao, B., & Ozaki, Y. (2004). Immunoassay using probe-labelling immunogold nanoparticles with silver staining enhancement via surface-enhanced Raman scattering. The Analyst, 129(1), 63-8.
Xu S, et al. Immunoassay Using Probe-labelling Immunogold Nanoparticles With Silver Staining Enhancement Via Surface-enhanced Raman Scattering. Analyst. 2004;129(1):63-8. PubMed PMID: 14737585.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoassay using probe-labelling immunogold nanoparticles with silver staining enhancement via surface-enhanced Raman scattering. AU - Xu,Shuping, AU - Ji,Xiaohui, AU - Xu,Weiqing, AU - Li,Xiaoling, AU - Wang,Lianying, AU - Bai,Yubai, AU - Zhao,Bing, AU - Ozaki,Yukihiro, Y1 - 2003/12/11/ PY - 2004/1/23/pubmed PY - 2004/4/17/medline PY - 2004/1/23/entrez SP - 63 EP - 8 JF - The Analyst JO - Analyst VL - 129 IS - 1 N2 - This paper reports a novel immunoassay based on surface-enhanced Raman scattering (SERS) and immunogold labelling with silver staining enhancement. Immunoreactions between immunogold colloids modified by a Raman-active probe molecule (e.g., 4-mercaptobenzoic acid) and antigens, which were captured by antibody-assembled chips such as silicon or quartz, were detected via SERS signals of Raman-active probe molecule. All the self-assembled steps were subjected to the measurements of ultraviolet-visible (UV-vis) spectra to monitor the formation of a sandwich structure onto a substrate. The immunoassay was performed by a sandwich structure consisting of three layers. The first layer was composed of immobilized antibody molecules of mouse polyclonal antibody against Hepatitis B virus surface antigen (PAb) on a silicon or quartz substrate. The second layer was the complementary Hepatitis B virus surface antigen (Antigen) molecules captured by PAb on the substrate. The third layer was composed of the probe-labelling immunogold nanoparticles, which were modified by mouse monoclonal antibody against Hepatitis B virus surface antigen (MAb) and 4-mercaptobenzoic acid (MBA) as the Raman-active probe on the surface of gold colloids. After silver staining enhancement, the antigen is identified by a SERS spectrum of MBA. A working curve of the intensity of a SERS signal at 1585 cm(-1) due to the [small nu](8a) aromatic ring vibration of MBA versus the concentration of analyte (Antigen) was obtained and the non-optimized detection limit for the Hepatitis B virus surface antigen was found to be as low as 0.5 [micro sign]g mL(-1). SN - 0003-2654 UR - https://www.unboundmedicine.com/medline/citation/14737585/Immunoassay_using_probe_labelling_immunogold_nanoparticles_with_silver_staining_enhancement_via_surface_enhanced_Raman_scattering_ L2 - https://doi.org/10.1039/b313094k DB - PRIME DP - Unbound Medicine ER -