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Molecular cloning and expression analysis of three genes encoding starch synthase II in rice.
Planta. 2004 Apr; 218(6):1062-70.P

Abstract

Three starch synthase (SS) genes, OsSSII-1, OsSSII-2 and OsSSII-3, were identified in rice (Oryza sativa L.) and localized to chromosomes 10, 2 and 6, respectively. The three OsSSII full-length cDNAs were cloned, and the predicted amino acid sequences were found to share 52-73% similarity with other members of the plant SSII family. The SS activity of each OsSSII was confirmed by expression and enzyme activity assay in Escherichia coli. Expression profile analysis revealed that OsSSII-1 was expressed in endosperms, leaves and roots; OsSSII-2 was mainly expressed in leaves, while OsSSII-3 was mainly expressed in endosperms. Similar to the OsSSI proteins, the OsSSII-2 and OsSSII-3 proteins were found in the soluble as well as the starch-granule-bound fractions in rice. The roles of the OsSSII proteins in starch biosynthesis in rice and the evolutionary relationships of the genes encoding monocotyledonous and dicotyledonous class-II SS enzymes are discussed.

Authors+Show Affiliations

Jiang H
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, 310029 Hangzhou, PR China.
Dian W
No affiliation info available
Liu F
No affiliation info available
Wu P
No affiliation info available

MeSH

Amino Acid SequenceChromosome MappingCloning, MolecularEscherichia coliGene ExpressionGene Expression Regulation, PlantMolecular Sequence DataOryzaPlant ProteinsRecombinant ProteinsSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidStarch Synthase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14740212

Citation

Jiang, Huawu, et al. "Molecular Cloning and Expression Analysis of Three Genes Encoding Starch Synthase II in Rice." Planta, vol. 218, no. 6, 2004, pp. 1062-70.
Jiang H, Dian W, Liu F, et al. Molecular cloning and expression analysis of three genes encoding starch synthase II in rice. Planta. 2004;218(6):1062-70.
Jiang, H., Dian, W., Liu, F., & Wu, P. (2004). Molecular cloning and expression analysis of three genes encoding starch synthase II in rice. Planta, 218(6), 1062-70.
Jiang H, et al. Molecular Cloning and Expression Analysis of Three Genes Encoding Starch Synthase II in Rice. Planta. 2004;218(6):1062-70. PubMed PMID: 14740212.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and expression analysis of three genes encoding starch synthase II in rice. AU - Jiang,Huawu, AU - Dian,Weimin, AU - Liu,Feiyang, AU - Wu,Ping, Y1 - 2004/01/23/ PY - 2003/10/11/received PY - 2003/12/12/accepted PY - 2004/1/24/pubmed PY - 2004/7/1/medline PY - 2004/1/24/entrez SP - 1062 EP - 70 JF - Planta JO - Planta VL - 218 IS - 6 N2 - Three starch synthase (SS) genes, OsSSII-1, OsSSII-2 and OsSSII-3, were identified in rice (Oryza sativa L.) and localized to chromosomes 10, 2 and 6, respectively. The three OsSSII full-length cDNAs were cloned, and the predicted amino acid sequences were found to share 52-73% similarity with other members of the plant SSII family. The SS activity of each OsSSII was confirmed by expression and enzyme activity assay in Escherichia coli. Expression profile analysis revealed that OsSSII-1 was expressed in endosperms, leaves and roots; OsSSII-2 was mainly expressed in leaves, while OsSSII-3 was mainly expressed in endosperms. Similar to the OsSSI proteins, the OsSSII-2 and OsSSII-3 proteins were found in the soluble as well as the starch-granule-bound fractions in rice. The roles of the OsSSII proteins in starch biosynthesis in rice and the evolutionary relationships of the genes encoding monocotyledonous and dicotyledonous class-II SS enzymes are discussed. SN - 0032-0935 UR - https://www.unboundmedicine.com/medline/citation/14740212/Molecular_cloning_and_expression_analysis_of_three_genes_encoding_starch_synthase_II_in_rice_ DB - PRIME DP - Unbound Medicine ER -