Tags

Type your tag names separated by a space and hit enter

Cannabinoid receptors and T helper cells.
J Neuroimmunol. 2004 Feb; 147(1-2):91-4.JN

Abstract

We have reported that injection of marijuana cannabinoids, such as Delta(9)-tetrahydrocannabinol (THC), into mice, followed by infection with Legionella pneumophila (Lp), suppresses the development of cell-mediated immunity T helper 1 (Th1) activity. These effects are accompanied by suppression of interleukin (IL)-12 and interferon (IFN) gamma production and enhancement of IL-4 production suggesting THC-induced T helper cell biasing. In the current report, other T helper cell biasing mechanisms were studied. Mice were injected with THC followed 18 h later by a challenge infection with Lp. Two-hour post-infection, spleens were removed and analyzed for mRNA to either IL-12Rbeta2 or GATA3 gene products. The results showed that THC suppressed IL-12Rbeta2 but increased GATA3. Receptor antagonists for CB1 (SR141716A, SR1) and CB2 (SR144528, SR2) were also injected to analyze the involvement of cannabinoid receptors. It was determined that SR1 attenuated the THC suppression of IL-12Rbeta2, while SR2 attenuated the increase in GATA3 mRNA. These results suggest that THC suppresses Th1 biasing activity such as IL-12Rbeta2 by a CB1 mediated mechanism and enhances the Th2 biasing activity, GATA3, by a CB2 mechanism. This dichotomy of receptor involvement might result from differential expression and/or signaling function of CB1 and CB2 on Th1 and Th2 cells.

Authors+Show Affiliations

Department of Medical Microbiology and Immunology, University of South Florida, 12901 Bruce Downs Boulevard, Tampa, FL 33612, USA. tklein@hsc.usf.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14741435

Citation

Klein, Thomas W., et al. "Cannabinoid Receptors and T Helper Cells." Journal of Neuroimmunology, vol. 147, no. 1-2, 2004, pp. 91-4.
Klein TW, Newton C, Larsen K, et al. Cannabinoid receptors and T helper cells. J Neuroimmunol. 2004;147(1-2):91-4.
Klein, T. W., Newton, C., Larsen, K., Chou, J., Perkins, I., Lu, L., Nong, L., & Friedman, H. (2004). Cannabinoid receptors and T helper cells. Journal of Neuroimmunology, 147(1-2), 91-4.
Klein TW, et al. Cannabinoid Receptors and T Helper Cells. J Neuroimmunol. 2004;147(1-2):91-4. PubMed PMID: 14741435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cannabinoid receptors and T helper cells. AU - Klein,Thomas W, AU - Newton,Cathy, AU - Larsen,Kellie, AU - Chou,Joe, AU - Perkins,Izabella, AU - Lu,Lily, AU - Nong,Liang, AU - Friedman,Herman, PY - 2004/1/27/pubmed PY - 2004/3/17/medline PY - 2004/1/27/entrez SP - 91 EP - 4 JF - Journal of neuroimmunology JO - J Neuroimmunol VL - 147 IS - 1-2 N2 - We have reported that injection of marijuana cannabinoids, such as Delta(9)-tetrahydrocannabinol (THC), into mice, followed by infection with Legionella pneumophila (Lp), suppresses the development of cell-mediated immunity T helper 1 (Th1) activity. These effects are accompanied by suppression of interleukin (IL)-12 and interferon (IFN) gamma production and enhancement of IL-4 production suggesting THC-induced T helper cell biasing. In the current report, other T helper cell biasing mechanisms were studied. Mice were injected with THC followed 18 h later by a challenge infection with Lp. Two-hour post-infection, spleens were removed and analyzed for mRNA to either IL-12Rbeta2 or GATA3 gene products. The results showed that THC suppressed IL-12Rbeta2 but increased GATA3. Receptor antagonists for CB1 (SR141716A, SR1) and CB2 (SR144528, SR2) were also injected to analyze the involvement of cannabinoid receptors. It was determined that SR1 attenuated the THC suppression of IL-12Rbeta2, while SR2 attenuated the increase in GATA3 mRNA. These results suggest that THC suppresses Th1 biasing activity such as IL-12Rbeta2 by a CB1 mediated mechanism and enhances the Th2 biasing activity, GATA3, by a CB2 mechanism. This dichotomy of receptor involvement might result from differential expression and/or signaling function of CB1 and CB2 on Th1 and Th2 cells. SN - 0165-5728 UR - https://www.unboundmedicine.com/medline/citation/14741435/Cannabinoid_receptors_and_T_helper_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165572803004387 DB - PRIME DP - Unbound Medicine ER -