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Selenium supplementation on plasma glutathione peroxidase activity in patients with end-stage chronic renal failure.
Biol Trace Elem Res. 2004 Jan; 97(1):15-30.BT

Abstract

Patients with chronic renal failure (CRF) usually have a lower than healthy level of selenium (Se) in whole blood and plasma. Plasma glutathione peroxidase (GSH-Px) is synthesized mostly in the kidney. In CRF patients, activity of this enzyme is significantly reduced and its reduction increases with the progress of the disease. The aim of the study was to evaluate the effect of Se supplementation to CRF patients at various stages of the disease on Se concentration in blood components and on plasma GSHPx activity. The study group comprised 53 CRF patients at various stages of the disease supplemented with Se (200 microg/d for 3 mo as Se-enriched yeast, containing about 70% L-selenomethionine [SeMet]). The control group consisted of 20 healthy subjects. The Se concentration in blood components was measured spectrofluorometrically with 2,3-diaminonaphthalene as a complexing reagent. GSH-Px activity in red cell hemolysates and plasma was assayed by the coupled method with tert-butyl hydroperoxide as a substrate. The Se concentration in whole blood and plasma of CRF patients is significantly lower as compared with healthy subjects, but similar at all stages of the disease. In the patients' plasma, total protein and albumin levels are also significantly lower than in healthy subjects. Plasma GSH-Px activity in patients is extremely low, and contrary to Se concentration, it decreases linearly with the increasing stage of the illness. Se-supplied patients show an increased Se concentration in all blood components and at all disease stages, whereas plasma GSH-Px activity is enhanced only at the incipient stage of the disease. Se supply has no effect on plasma GSHPx activity in uremic patients at the end stage of the disease. Total plasma protein and albumin levels did not change after Se supplementation. Our data seem to show that in patients with CRF lower total protein and albumin levels in plasma may be the chief cause of the low blood and plasma Se concentrations. GSH-Px activity decreases along with the kidney impairment. At the end stage of the disease, Se supplementation in the form of Se-enriched yeast has no effect on the increase in plasma GSH-Px activity.

Authors+Show Affiliations

Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Lodz, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

14742897

Citation

Zachara, Bronisław A., et al. "Selenium Supplementation On Plasma Glutathione Peroxidase Activity in Patients With End-stage Chronic Renal Failure." Biological Trace Element Research, vol. 97, no. 1, 2004, pp. 15-30.
Zachara BA, Koterska D, Manitius J, et al. Selenium supplementation on plasma glutathione peroxidase activity in patients with end-stage chronic renal failure. Biol Trace Elem Res. 2004;97(1):15-30.
Zachara, B. A., Koterska, D., Manitius, J., Sadowski, L., Dziedziczko, A., Salak, A., & Wasowicz, W. (2004). Selenium supplementation on plasma glutathione peroxidase activity in patients with end-stage chronic renal failure. Biological Trace Element Research, 97(1), 15-30.
Zachara BA, et al. Selenium Supplementation On Plasma Glutathione Peroxidase Activity in Patients With End-stage Chronic Renal Failure. Biol Trace Elem Res. 2004;97(1):15-30. PubMed PMID: 14742897.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selenium supplementation on plasma glutathione peroxidase activity in patients with end-stage chronic renal failure. AU - Zachara,Bronisław A, AU - Koterska,Dominika, AU - Manitius,Jacek, AU - Sadowski,Leszek, AU - Dziedziczko,Andrzej, AU - Salak,Anna, AU - Wasowicz,Wojciech, PY - 2003/03/17/received PY - 2003/05/13/revised PY - 2003/06/17/accepted PY - 2004/1/27/pubmed PY - 2004/9/8/medline PY - 2004/1/27/entrez SP - 15 EP - 30 JF - Biological trace element research JO - Biol Trace Elem Res VL - 97 IS - 1 N2 - Patients with chronic renal failure (CRF) usually have a lower than healthy level of selenium (Se) in whole blood and plasma. Plasma glutathione peroxidase (GSH-Px) is synthesized mostly in the kidney. In CRF patients, activity of this enzyme is significantly reduced and its reduction increases with the progress of the disease. The aim of the study was to evaluate the effect of Se supplementation to CRF patients at various stages of the disease on Se concentration in blood components and on plasma GSHPx activity. The study group comprised 53 CRF patients at various stages of the disease supplemented with Se (200 microg/d for 3 mo as Se-enriched yeast, containing about 70% L-selenomethionine [SeMet]). The control group consisted of 20 healthy subjects. The Se concentration in blood components was measured spectrofluorometrically with 2,3-diaminonaphthalene as a complexing reagent. GSH-Px activity in red cell hemolysates and plasma was assayed by the coupled method with tert-butyl hydroperoxide as a substrate. The Se concentration in whole blood and plasma of CRF patients is significantly lower as compared with healthy subjects, but similar at all stages of the disease. In the patients' plasma, total protein and albumin levels are also significantly lower than in healthy subjects. Plasma GSH-Px activity in patients is extremely low, and contrary to Se concentration, it decreases linearly with the increasing stage of the illness. Se-supplied patients show an increased Se concentration in all blood components and at all disease stages, whereas plasma GSH-Px activity is enhanced only at the incipient stage of the disease. Se supply has no effect on plasma GSHPx activity in uremic patients at the end stage of the disease. Total plasma protein and albumin levels did not change after Se supplementation. Our data seem to show that in patients with CRF lower total protein and albumin levels in plasma may be the chief cause of the low blood and plasma Se concentrations. GSH-Px activity decreases along with the kidney impairment. At the end stage of the disease, Se supplementation in the form of Se-enriched yeast has no effect on the increase in plasma GSH-Px activity. SN - 0163-4984 UR - https://www.unboundmedicine.com/medline/citation/14742897/Selenium_supplementation_on_plasma_glutathione_peroxidase_activity_in_patients_with_end_stage_chronic_renal_failure_ L2 - https://dx.doi.org/10.1385/BTER:97:1:15 DB - PRIME DP - Unbound Medicine ER -