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Mating type-dependent constraints on the mobility of the left arm of yeast chromosome III.
J Cell Biol. 2004 Feb 02; 164(3):361-71.JC

Abstract

Mating-type gene (MAT) switching in budding yeast exhibits donor preference. MATa preferentially recombines with HML near the left telomere of chromosome III, whereas MATalpha prefers HMR near the right telomere. Donor preference is controlled by the recombination enhancer (RE) located proximal to HML. To test if HML is constrained in pairing with MATalpha, we examined live-cell mobility of LacI-GFP-bound lactose operator (lacO) arrays inserted at different chromosomal sites. Without induction of recombination, lacO sequences adjacent to HML are strongly constrained in both MATalpha and RE-deleted MATa strains, compared with MATa. In contrast, chromosome movement at HMR or near a telomere of chromosome V is mating-type independent. HML is more constrained in MATa Deltare and less constrained in MATa RE+ compared with other sites. Although HML and MATa are not prealigned before inducing recombination, the three-dimensional configuration of MAT, HML, and HMR is mating-type dependent. These data suggest there is constitutive tethering of HML, which is relieved in MATa cells through the action of RE.

Authors+Show Affiliations

Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454-9910, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14745000

Citation

Bressan, Debra A., et al. "Mating Type-dependent Constraints On the Mobility of the Left Arm of Yeast Chromosome III." The Journal of Cell Biology, vol. 164, no. 3, 2004, pp. 361-71.
Bressan DA, Vazquez J, Haber JE. Mating type-dependent constraints on the mobility of the left arm of yeast chromosome III. J Cell Biol. 2004;164(3):361-71.
Bressan, D. A., Vazquez, J., & Haber, J. E. (2004). Mating type-dependent constraints on the mobility of the left arm of yeast chromosome III. The Journal of Cell Biology, 164(3), 361-71.
Bressan DA, Vazquez J, Haber JE. Mating Type-dependent Constraints On the Mobility of the Left Arm of Yeast Chromosome III. J Cell Biol. 2004 Feb 2;164(3):361-71. PubMed PMID: 14745000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mating type-dependent constraints on the mobility of the left arm of yeast chromosome III. AU - Bressan,Debra A, AU - Vazquez,Julio, AU - Haber,James E, Y1 - 2004/01/26/ PY - 2004/1/28/pubmed PY - 2004/9/11/medline PY - 2004/1/28/entrez SP - 361 EP - 71 JF - The Journal of cell biology JO - J Cell Biol VL - 164 IS - 3 N2 - Mating-type gene (MAT) switching in budding yeast exhibits donor preference. MATa preferentially recombines with HML near the left telomere of chromosome III, whereas MATalpha prefers HMR near the right telomere. Donor preference is controlled by the recombination enhancer (RE) located proximal to HML. To test if HML is constrained in pairing with MATalpha, we examined live-cell mobility of LacI-GFP-bound lactose operator (lacO) arrays inserted at different chromosomal sites. Without induction of recombination, lacO sequences adjacent to HML are strongly constrained in both MATalpha and RE-deleted MATa strains, compared with MATa. In contrast, chromosome movement at HMR or near a telomere of chromosome V is mating-type independent. HML is more constrained in MATa Deltare and less constrained in MATa RE+ compared with other sites. Although HML and MATa are not prealigned before inducing recombination, the three-dimensional configuration of MAT, HML, and HMR is mating-type dependent. These data suggest there is constitutive tethering of HML, which is relieved in MATa cells through the action of RE. SN - 0021-9525 UR - https://www.unboundmedicine.com/medline/citation/14745000/Mating_type_dependent_constraints_on_the_mobility_of_the_left_arm_of_yeast_chromosome_III_ L2 - https://rupress.org/jcb/article-lookup/doi/10.1083/jcb.200311063 DB - PRIME DP - Unbound Medicine ER -