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New insight into progenitor/stem cells in dental pulp using Col1a1-GFP transgenes.
Cells Tissues Organs. 2004; 176(1-3):120-33.CT

Abstract

In recent years there has been increasing progress in identifying stem cells from adult tissues and their potential application in tissue engineering. These advances provide a promising future for tooth replacement/regeneration. Essential for this approach is the identification of donor stem cells for various components of the teeth. Our studies show that pOBCol3.6GFPtpz and pOBCol2.3GFPemd transgenic animals provide a unique model to gain insight into stem cells in the dental pulp. Our in vivo studies of the developing teeth of these transgenic lines show both Col1a1-GFP transgenes are expressed in functional and fully differentiated odontoblasts. The patterns of expression of Col1a1-GFP transgenes during odontoblast differentiation correlates with the expression of DSPP. In the developing craniofacial bones both Col1a1-GFP transgenes are also expressed in osteoblasts and osteocytes of alveolar and calvarial bones. In the alveolar bones, the expression of Col1a1-GFP in osteocytes correlates with the expression of DMP1. Col1a1-3.6-GFP is expressed in the entire layer of the periosteum and in suture mesenchyme containing osteoprogenitor cells. On the other hand, Col1a1-2.3- GFP expression was limited to the osteoblastic layer of the periosteum and was not detected in the fibroblastic layer of the periosteum or in the suture mesenchyme. These observations indicate that Col1a1-3.6-GFP and Col1a1-2.3-GFP transgenes identify different subpopulations of cells during intramembranous ossification. By using the coronal portion of dental pulps isolated from postnatal transgenic mice our observations also provide direct evidence that the dental pulp contains progenitor/stem cells capable of giving rise to a new generation of odontoblast-like cells, as well as osteoblast-like cells.

Authors+Show Affiliations

Department of Pediatric Dentistry, School of Dental Medicine, University of Connecticut Health Center, Farmington, Conn., USA. mina@nso1.uchc.eduNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14745241

Citation

Mina, Mina, and Alen Braut. "New Insight Into Progenitor/stem Cells in Dental Pulp Using Col1a1-GFP Transgenes." Cells, Tissues, Organs, vol. 176, no. 1-3, 2004, pp. 120-33.
Mina M, Braut A. New insight into progenitor/stem cells in dental pulp using Col1a1-GFP transgenes. Cells Tissues Organs (Print). 2004;176(1-3):120-33.
Mina, M., & Braut, A. (2004). New insight into progenitor/stem cells in dental pulp using Col1a1-GFP transgenes. Cells, Tissues, Organs, 176(1-3), 120-33.
Mina M, Braut A. New Insight Into Progenitor/stem Cells in Dental Pulp Using Col1a1-GFP Transgenes. Cells Tissues Organs (Print). 2004;176(1-3):120-33. PubMed PMID: 14745241.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New insight into progenitor/stem cells in dental pulp using Col1a1-GFP transgenes. AU - Mina,Mina, AU - Braut,Alen, PY - 2004/1/28/pubmed PY - 2004/9/21/medline PY - 2004/1/28/entrez SP - 120 EP - 33 JF - Cells, tissues, organs JO - Cells Tissues Organs (Print) VL - 176 IS - 1-3 N2 - In recent years there has been increasing progress in identifying stem cells from adult tissues and their potential application in tissue engineering. These advances provide a promising future for tooth replacement/regeneration. Essential for this approach is the identification of donor stem cells for various components of the teeth. Our studies show that pOBCol3.6GFPtpz and pOBCol2.3GFPemd transgenic animals provide a unique model to gain insight into stem cells in the dental pulp. Our in vivo studies of the developing teeth of these transgenic lines show both Col1a1-GFP transgenes are expressed in functional and fully differentiated odontoblasts. The patterns of expression of Col1a1-GFP transgenes during odontoblast differentiation correlates with the expression of DSPP. In the developing craniofacial bones both Col1a1-GFP transgenes are also expressed in osteoblasts and osteocytes of alveolar and calvarial bones. In the alveolar bones, the expression of Col1a1-GFP in osteocytes correlates with the expression of DMP1. Col1a1-3.6-GFP is expressed in the entire layer of the periosteum and in suture mesenchyme containing osteoprogenitor cells. On the other hand, Col1a1-2.3- GFP expression was limited to the osteoblastic layer of the periosteum and was not detected in the fibroblastic layer of the periosteum or in the suture mesenchyme. These observations indicate that Col1a1-3.6-GFP and Col1a1-2.3-GFP transgenes identify different subpopulations of cells during intramembranous ossification. By using the coronal portion of dental pulps isolated from postnatal transgenic mice our observations also provide direct evidence that the dental pulp contains progenitor/stem cells capable of giving rise to a new generation of odontoblast-like cells, as well as osteoblast-like cells. SN - 1422-6405 UR - https://www.unboundmedicine.com/medline/citation/14745241/New_insight_into_progenitor/stem_cells_in_dental_pulp_using_Col1a1_GFP_transgenes_ L2 - https://www.karger.com?DOI=10.1159/000075033 DB - PRIME DP - Unbound Medicine ER -