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Quantitative analysis of triacylglycerol regioisomers in fats and oils using reversed-phase high-performance liquid chromatography and atmospheric pressure chemical ionization mass spectrometry.
Rapid Commun Mass Spectrom. 2004; 18(2):218-24.RC

Abstract

Positional distribution of fatty acyl chains of triacylglycerols (TGs) in vegetable oils and fats (palm oil, cocoa butter) and animal fats (beef, pork and chicken fats) was examined by reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to atmospheric pressure chemical ionization using a quadrupole mass spectrometer. Quantification of regioisomers was achieved for TGs containing two different fatty acyl chains (palmitic (P), stearic (S), oleic (O), and/or linoleic (L)). For seven pairs of 'AAB/ABA'-type TGs, namely PPS/PSP, PPO/POP, SSO/SOS, POO/OPO, SOO/OSO, PPL/PLP and LLS/LSL, calibration curves were established on the basis of the difference in relative abundances of the fragment ions produced by preferred losses of the fatty acid from the 1/3-position compared to the 2-position. In practice the positional isomers AAB and ABA yield mass spectra showing a significant difference in relative abundance ratios of the ions AA(+) to AB(+). Statistical analysis of the validation data obtained from analysis of TG standards and spiked oils showed that, under repeatability conditions, least-squares regression can be used to establish calibration curves for all pairs. The regression models show linear behavior that allow the determination of the proportion of each regioisomer in an AAB/ABA pair, within a working range from 10 to 1000 microg/mL and a 95% confidence interval of +/-3% for three replicates.

Authors+Show Affiliations

Fauconnot L
Nestlé Research Center, Nestec Ltd. Vers-chez-les-Blanc, P.O. Box 44, CH-1000 Lausanne 26, Switzerland. laetitia.fauconnot@rdls.nestle.com
Hau J
No affiliation info available
Aeschlimann JM
No affiliation info available
Fay LB
No affiliation info available
Dionisi F
No affiliation info available

MeSH

AnimalsAtmospheric PressureCalibrationChromatography, High Pressure LiquidFatsIsomerismMass SpectrometryMeatOilsReference StandardsTriglycerides

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14745773

Citation

Fauconnot, Laëtitia, et al. "Quantitative Analysis of Triacylglycerol Regioisomers in Fats and Oils Using Reversed-phase High-performance Liquid Chromatography and Atmospheric Pressure Chemical Ionization Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 18, no. 2, 2004, pp. 218-24.
Fauconnot L, Hau J, Aeschlimann JM, et al. Quantitative analysis of triacylglycerol regioisomers in fats and oils using reversed-phase high-performance liquid chromatography and atmospheric pressure chemical ionization mass spectrometry. Rapid Commun Mass Spectrom. 2004;18(2):218-24.
Fauconnot, L., Hau, J., Aeschlimann, J. M., Fay, L. B., & Dionisi, F. (2004). Quantitative analysis of triacylglycerol regioisomers in fats and oils using reversed-phase high-performance liquid chromatography and atmospheric pressure chemical ionization mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 18(2), 218-24.
Fauconnot L, et al. Quantitative Analysis of Triacylglycerol Regioisomers in Fats and Oils Using Reversed-phase High-performance Liquid Chromatography and Atmospheric Pressure Chemical Ionization Mass Spectrometry. Rapid Commun Mass Spectrom. 2004;18(2):218-24. PubMed PMID: 14745773.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative analysis of triacylglycerol regioisomers in fats and oils using reversed-phase high-performance liquid chromatography and atmospheric pressure chemical ionization mass spectrometry. AU - Fauconnot,Laëtitia, AU - Hau,Jörg, AU - Aeschlimann,Jean-Marc, AU - Fay,Laurent-Bernard, AU - Dionisi,Fabiola, PY - 2004/1/28/pubmed PY - 2004/3/27/medline PY - 2004/1/28/entrez SP - 218 EP - 24 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 18 IS - 2 N2 - Positional distribution of fatty acyl chains of triacylglycerols (TGs) in vegetable oils and fats (palm oil, cocoa butter) and animal fats (beef, pork and chicken fats) was examined by reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to atmospheric pressure chemical ionization using a quadrupole mass spectrometer. Quantification of regioisomers was achieved for TGs containing two different fatty acyl chains (palmitic (P), stearic (S), oleic (O), and/or linoleic (L)). For seven pairs of 'AAB/ABA'-type TGs, namely PPS/PSP, PPO/POP, SSO/SOS, POO/OPO, SOO/OSO, PPL/PLP and LLS/LSL, calibration curves were established on the basis of the difference in relative abundances of the fragment ions produced by preferred losses of the fatty acid from the 1/3-position compared to the 2-position. In practice the positional isomers AAB and ABA yield mass spectra showing a significant difference in relative abundance ratios of the ions AA(+) to AB(+). Statistical analysis of the validation data obtained from analysis of TG standards and spiked oils showed that, under repeatability conditions, least-squares regression can be used to establish calibration curves for all pairs. The regression models show linear behavior that allow the determination of the proportion of each regioisomer in an AAB/ABA pair, within a working range from 10 to 1000 microg/mL and a 95% confidence interval of +/-3% for three replicates. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/14745773/Quantitative_analysis_of_triacylglycerol_regioisomers_in_fats_and_oils_using_reversed_phase_high_performance_liquid_chromatography_and_atmospheric_pressure_chemical_ionization_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -
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