Tags

Type your tag names separated by a space and hit enter

Endothelium-specific Cre recombinase activity in flk-1-Cre transgenic mice.
Dev Dyn. 2004 Feb; 229(2):312-8.DD

Abstract

The use of the Cre-loxP recombination system allows the conditional inactivation of genes in mice. The availability of transgenic mice in which the Cre recombinase expression is highly cell type specific is a prerequisite to successfully use this system. We previously have characterized regulatory regions of the mouse flk-1 gene sufficient for endothelial cell-specific expression of the LacZ reporter gene in transgenic mice. These regions were fused to the Cre recombinase gene, and transgenic mouse lines were generated. In the resulting flk-1-Cre transgenic mice, specificity of Cre activity was determined by cross-breeding with the reporter mouse lines Rosa26R or CAG-CAT-LacZ. We examined double-transgenic mice at different stages of embryonic development (E9.5-E16.5) and organs of adult animals by LacZ staining. Strong endothelium-specific staining of most vascular beds was observed in embryos older than E11.5 in one or E13.5 in a second line. In addition, the neovasculature of experimental BFS-1 tumors expressed the transgene. These lines will be valuable for the conditional inactivation of floxed target genes in endothelial cells of the embryonic vascular system.

Authors+Show Affiliations

Department of Molecular Cell Biology, Max Planck Institute for Physiological and Clinical Research, Bad Nauheim, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14745955

Citation

Licht, Alexander H., et al. "Endothelium-specific Cre Recombinase Activity in flk-1-Cre Transgenic Mice." Developmental Dynamics : an Official Publication of the American Association of Anatomists, vol. 229, no. 2, 2004, pp. 312-8.
Licht AH, Raab S, Hofmann U, et al. Endothelium-specific Cre recombinase activity in flk-1-Cre transgenic mice. Dev Dyn. 2004;229(2):312-8.
Licht, A. H., Raab, S., Hofmann, U., & Breier, G. (2004). Endothelium-specific Cre recombinase activity in flk-1-Cre transgenic mice. Developmental Dynamics : an Official Publication of the American Association of Anatomists, 229(2), 312-8.
Licht AH, et al. Endothelium-specific Cre Recombinase Activity in flk-1-Cre Transgenic Mice. Dev Dyn. 2004;229(2):312-8. PubMed PMID: 14745955.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endothelium-specific Cre recombinase activity in flk-1-Cre transgenic mice. AU - Licht,Alexander H, AU - Raab,Sabine, AU - Hofmann,Ursula, AU - Breier,Georg, PY - 2004/1/28/pubmed PY - 2004/9/21/medline PY - 2004/1/28/entrez SP - 312 EP - 8 JF - Developmental dynamics : an official publication of the American Association of Anatomists JO - Dev Dyn VL - 229 IS - 2 N2 - The use of the Cre-loxP recombination system allows the conditional inactivation of genes in mice. The availability of transgenic mice in which the Cre recombinase expression is highly cell type specific is a prerequisite to successfully use this system. We previously have characterized regulatory regions of the mouse flk-1 gene sufficient for endothelial cell-specific expression of the LacZ reporter gene in transgenic mice. These regions were fused to the Cre recombinase gene, and transgenic mouse lines were generated. In the resulting flk-1-Cre transgenic mice, specificity of Cre activity was determined by cross-breeding with the reporter mouse lines Rosa26R or CAG-CAT-LacZ. We examined double-transgenic mice at different stages of embryonic development (E9.5-E16.5) and organs of adult animals by LacZ staining. Strong endothelium-specific staining of most vascular beds was observed in embryos older than E11.5 in one or E13.5 in a second line. In addition, the neovasculature of experimental BFS-1 tumors expressed the transgene. These lines will be valuable for the conditional inactivation of floxed target genes in endothelial cells of the embryonic vascular system. SN - 1058-8388 UR - https://www.unboundmedicine.com/medline/citation/14745955/Endothelium_specific_Cre_recombinase_activity_in_flk_1_Cre_transgenic_mice_ L2 - https://doi.org/10.1002/dvdy.10416 DB - PRIME DP - Unbound Medicine ER -