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Dependence of endocochlear potential on basolateral Na+ and Cl- concentration: a study using vascular and perilymph perfusion.
Jpn J Physiol. 1992; 42(4):617-30.JJ

Abstract

Inside-positive endocochlear potential (EP) and high potassium concentration in the endocochlear duct are generated by transepithelial K+ transport in marginal cells of the stria vascularis. In order to estimate the degree of involvement of Na+ and Cl- in K+ transport in marginal cells, EP in guinea pigs was measured under artificial vascular and perilymphatic perfusion in situ. Na+ depletion due to both vascular and perilymphatic perfusion decreased EP by -10.0 +/- 4.1 mV (delta EP = -86 +/- 5.2 mV, n = 5) from the control value of 78 +/- 4.3 mV (p < 0.01). Cl- depletion due to vascular and perilymphatic perfusion also decreased EP by -10.0 +/- 4.9 mV (delta EP = 8.5 +/- 4.8 mV, n = 6) from the control value of 77 +/- 5.1 mV (p < 0.01). However, under either vascular or perilymphatic perfusion, even lowering of Na+ or Cl- concentration in the perfusate decreased EP only slightly compared to the results under both vascular and perilymphatic perfusion. Furosemide, a blocker of Na+/K+/2Cl- symport, decreased EP under vascular perfusion. This dependency of EP on basolateral Na+ and Cl- concentration strongly suggests that K+ transport by the marginal cell is dependent on the basolateral Na+ and Cl- concentration, and that Na+/K+/2Cl- symport is raised as a possible mechanism for Na+ and Cl- dependency of EP.

Authors+Show Affiliations

Department of Otorhinolaryngology, Kyoto Prefectural Medical University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1474679

Citation

Shindo, M, et al. "Dependence of Endocochlear Potential On Basolateral Na+ and Cl- Concentration: a Study Using Vascular and Perilymph Perfusion." The Japanese Journal of Physiology, vol. 42, no. 4, 1992, pp. 617-30.
Shindo M, Miyamoto M, Abe N, et al. Dependence of endocochlear potential on basolateral Na+ and Cl- concentration: a study using vascular and perilymph perfusion. Jpn J Physiol. 1992;42(4):617-30.
Shindo, M., Miyamoto, M., Abe, N., Shida, S., Murakami, Y., & Imai, Y. (1992). Dependence of endocochlear potential on basolateral Na+ and Cl- concentration: a study using vascular and perilymph perfusion. The Japanese Journal of Physiology, 42(4), 617-30.
Shindo M, et al. Dependence of Endocochlear Potential On Basolateral Na+ and Cl- Concentration: a Study Using Vascular and Perilymph Perfusion. Jpn J Physiol. 1992;42(4):617-30. PubMed PMID: 1474679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dependence of endocochlear potential on basolateral Na+ and Cl- concentration: a study using vascular and perilymph perfusion. AU - Shindo,M, AU - Miyamoto,M, AU - Abe,N, AU - Shida,S, AU - Murakami,Y, AU - Imai,Y, PY - 1992/1/1/pubmed PY - 1992/1/1/medline PY - 1992/1/1/entrez SP - 617 EP - 30 JF - The Japanese journal of physiology JO - Jpn J Physiol VL - 42 IS - 4 N2 - Inside-positive endocochlear potential (EP) and high potassium concentration in the endocochlear duct are generated by transepithelial K+ transport in marginal cells of the stria vascularis. In order to estimate the degree of involvement of Na+ and Cl- in K+ transport in marginal cells, EP in guinea pigs was measured under artificial vascular and perilymphatic perfusion in situ. Na+ depletion due to both vascular and perilymphatic perfusion decreased EP by -10.0 +/- 4.1 mV (delta EP = -86 +/- 5.2 mV, n = 5) from the control value of 78 +/- 4.3 mV (p < 0.01). Cl- depletion due to vascular and perilymphatic perfusion also decreased EP by -10.0 +/- 4.9 mV (delta EP = 8.5 +/- 4.8 mV, n = 6) from the control value of 77 +/- 5.1 mV (p < 0.01). However, under either vascular or perilymphatic perfusion, even lowering of Na+ or Cl- concentration in the perfusate decreased EP only slightly compared to the results under both vascular and perilymphatic perfusion. Furosemide, a blocker of Na+/K+/2Cl- symport, decreased EP under vascular perfusion. This dependency of EP on basolateral Na+ and Cl- concentration strongly suggests that K+ transport by the marginal cell is dependent on the basolateral Na+ and Cl- concentration, and that Na+/K+/2Cl- symport is raised as a possible mechanism for Na+ and Cl- dependency of EP. SN - 0021-521X UR - https://www.unboundmedicine.com/medline/citation/1474679/Dependence_of_endocochlear_potential_on_basolateral_Na+_and_Cl__concentration:_a_study_using_vascular_and_perilymph_perfusion_ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/jjphysiol/42.617?from=PubMed DB - PRIME DP - Unbound Medicine ER -