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High frequency of defective sperm-zona pellucida interaction in oligozoospermic infertile men.
Hum Reprod 2004; 19(2):228-33HR

Abstract

BACKGROUND

The ability of sperm to interact with the zona pellucida (ZP) plays a critical role during the process of human fertilization. The aim of this study is to determine frequency of defective sperm-ZP interaction in oligozoospermic infertile men.

METHODS

Sperm-ZP binding assays and the ZP-induced acrosome reaction (AR) were performed in 72 infertile men with a sperm concentration <20 x 10(6)/ml. Oocytes that had previously failed to fertilize in a clinical IVF programme were used for the tests. Motile sperm (2 x 10(6)/ml) selected by swim-up from each semen sample were incubated with four oocytes for 2 h. The number of sperm bound per ZP and the ZP-induced AR were assessed. Under these conditions, an average of < or =40 sperm bound/ZP was defined as low sperm-ZP binding and a ZP-induced AR < or =16% was defined as low ZP-induced AR.

RESULTS

In the 72 oligozoospermic men, 28% (20/72) had low sperm-ZP binding. Of those with normal sperm-ZP binding, 69% (36/52) had low ZP-induced AR. Overall, 78% (56/72) had either low ZP-binding or normal ZP binding but low ZP-induced AR. This means that only 22% (16/72) had both normal sperm-ZP binding and normal ZP-induced AR.

CONCLUSION

Oligozoospermic men have a very high frequency of defective sperm-ZP interaction, consistent with their low natural fertility or low fertilization rate in conventional IVF. Infertile couples with oligozoospermic semen should be treated by ICSI rather than by conventional IVF.

Authors+Show Affiliations

University of Melbourne Department of Obstetrics and Gynaecology, Reproductive Services, Royal Women's Hospital and Melbourne IVF, Victoria 3035, Australia. dyl@unimelb.edu.auNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14747159

Citation

Liu, De Yi, and H W Gordon Baker. "High Frequency of Defective Sperm-zona Pellucida Interaction in Oligozoospermic Infertile Men." Human Reproduction (Oxford, England), vol. 19, no. 2, 2004, pp. 228-33.
Liu DY, Baker HW. High frequency of defective sperm-zona pellucida interaction in oligozoospermic infertile men. Hum Reprod. 2004;19(2):228-33.
Liu, D. Y., & Baker, H. W. (2004). High frequency of defective sperm-zona pellucida interaction in oligozoospermic infertile men. Human Reproduction (Oxford, England), 19(2), pp. 228-33.
Liu DY, Baker HW. High Frequency of Defective Sperm-zona Pellucida Interaction in Oligozoospermic Infertile Men. Hum Reprod. 2004;19(2):228-33. PubMed PMID: 14747159.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High frequency of defective sperm-zona pellucida interaction in oligozoospermic infertile men. AU - Liu,De Yi, AU - Baker,H W Gordon, PY - 2004/1/30/pubmed PY - 2004/9/25/medline PY - 2004/1/30/entrez SP - 228 EP - 33 JF - Human reproduction (Oxford, England) JO - Hum. Reprod. VL - 19 IS - 2 N2 - BACKGROUND: The ability of sperm to interact with the zona pellucida (ZP) plays a critical role during the process of human fertilization. The aim of this study is to determine frequency of defective sperm-ZP interaction in oligozoospermic infertile men. METHODS: Sperm-ZP binding assays and the ZP-induced acrosome reaction (AR) were performed in 72 infertile men with a sperm concentration <20 x 10(6)/ml. Oocytes that had previously failed to fertilize in a clinical IVF programme were used for the tests. Motile sperm (2 x 10(6)/ml) selected by swim-up from each semen sample were incubated with four oocytes for 2 h. The number of sperm bound per ZP and the ZP-induced AR were assessed. Under these conditions, an average of < or =40 sperm bound/ZP was defined as low sperm-ZP binding and a ZP-induced AR < or =16% was defined as low ZP-induced AR. RESULTS: In the 72 oligozoospermic men, 28% (20/72) had low sperm-ZP binding. Of those with normal sperm-ZP binding, 69% (36/52) had low ZP-induced AR. Overall, 78% (56/72) had either low ZP-binding or normal ZP binding but low ZP-induced AR. This means that only 22% (16/72) had both normal sperm-ZP binding and normal ZP-induced AR. CONCLUSION: Oligozoospermic men have a very high frequency of defective sperm-ZP interaction, consistent with their low natural fertility or low fertilization rate in conventional IVF. Infertile couples with oligozoospermic semen should be treated by ICSI rather than by conventional IVF. SN - 0268-1161 UR - https://www.unboundmedicine.com/medline/citation/14747159/High_frequency_of_defective_sperm_zona_pellucida_interaction_in_oligozoospermic_infertile_men_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/deh067 DB - PRIME DP - Unbound Medicine ER -