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Expression, purification, crystallization and preliminary crystallographic analysis of osmotically inducible protein C.
Acta Crystallogr D Biol Crystallogr. 2004 Feb; 60(Pt 2):357-8.AC

Abstract

Selenium-incorporated osmotically inducible protein C from the thermophilic bacterium Thermus thermophilus was overexpressed, purified and crystallized. The crystals belong to space group P1, with unit-cell parameters a = 37.58, b = 40.95, c = 48.14 A, alpha = 76.93, beta = 74.04, gamma = 64.05 degrees. Five data sets were collected from a single crystal to 1.6 A using synchrotron radiation for MAD phasing. Self-rotation functions and the Matthews coefficient are consistent with two molecules in the asymmetric unit.

Authors+Show Affiliations

Rehse PH
Highthroughput Factory, RIKEN Harima Institute, 1-1-1 Kouto, Mikazuki-cho, Sayo-gun, Hyogo 679-5148, Japan.
Nodake Y
No affiliation info available
Kuroishi C
No affiliation info available
Tahirov TH
No affiliation info available

MeSH

CrystallographyCrystallography, X-RayDatabases as TopicEscherichia coliPolymerase Chain ReactionProtein CSeleniumSynchrotronsTemperatureThermus thermophilus

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14747724

Citation

Rehse, Peter H., et al. "Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of Osmotically Inducible Protein C." Acta Crystallographica. Section D, Biological Crystallography, vol. 60, no. Pt 2, 2004, pp. 357-8.
Rehse PH, Nodake Y, Kuroishi C, et al. Expression, purification, crystallization and preliminary crystallographic analysis of osmotically inducible protein C. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 2):357-8.
Rehse, P. H., Nodake, Y., Kuroishi, C., & Tahirov, T. H. (2004). Expression, purification, crystallization and preliminary crystallographic analysis of osmotically inducible protein C. Acta Crystallographica. Section D, Biological Crystallography, 60(Pt 2), 357-8.
Rehse PH, et al. Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of Osmotically Inducible Protein C. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 2):357-8. PubMed PMID: 14747724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression, purification, crystallization and preliminary crystallographic analysis of osmotically inducible protein C. AU - Rehse,Peter H, AU - Nodake,Yuichi, AU - Kuroishi,Chizu, AU - Tahirov,Tahir H, Y1 - 2004/01/23/ PY - 2003/10/10/received PY - 2003/12/01/accepted PY - 2004/1/30/pubmed PY - 2004/10/9/medline PY - 2004/1/30/entrez SP - 357 EP - 8 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr D Biol Crystallogr VL - 60 IS - Pt 2 N2 - Selenium-incorporated osmotically inducible protein C from the thermophilic bacterium Thermus thermophilus was overexpressed, purified and crystallized. The crystals belong to space group P1, with unit-cell parameters a = 37.58, b = 40.95, c = 48.14 A, alpha = 76.93, beta = 74.04, gamma = 64.05 degrees. Five data sets were collected from a single crystal to 1.6 A using synchrotron radiation for MAD phasing. Self-rotation functions and the Matthews coefficient are consistent with two molecules in the asymmetric unit. SN - 0907-4449 UR - https://www.unboundmedicine.com/medline/citation/14747724/Expression_purification_crystallization_and_preliminary_crystallographic_analysis_of_osmotically_inducible_protein_C_ DB - PRIME DP - Unbound Medicine ER -