Tags

Type your tag names separated by a space and hit enter

Cloning and characterization of a gamma-3 hordein mRNA (cDNA) from Hordeum chilense (Roem. et Schult.).
Theor Appl Genet. 2004 May; 108(7):1359-65.TA

Abstract

Hordeum chilense is a wild relative of H. vulgare, cultivated barley, that has been successfully used in the synthesis of amphiploids by crossing with Triticum spp. These amphiploids-named generically x Tritordeum-have been tested under field conditions, and one of them, the hexaploid tritordeum obtained following chromosome doubling of the hybrid H. chilense x T. turgidum, shows traits of interest inherited from the barley parent. Of great interest is the allelic variation observed in the endosperm storage proteins and their influence on the breadmaking and malting quality of tritordeum. We report here two mRNA (cDNA) sequences for a gamma-3 hordein from two accession lines of H. chilense, H1 and H7, and their characterization by quantitative real time (QRT)-PCR in the developing endosperm. Sequences were obtained by rapid amplification of cDNA ends and "edge-to-edge" amplification of open reading frames from cDNA of H. chilense. Eight putative single nucleotide polymorphisms and one codon insertion were identified in the sequences of the H1 and H7 gamma-3 hordeins. The deduced amino acid sequences showed similar features to that of the gamma-3 hordein and gamma-gliadins from barley and wheat, respectively. While the repetitive motif (PQQQPF) is similar to that of the gamma-3 hordein from H. vulgare, there are 19 motif repeats in H. vulgare, whereas H. chilense shows 15 tandem repeats. The transcription of the genes encoding for the gamma-3 hordein were monitored by QRT-PCR: in both lines maximum transcription occurred 12 days after flowering.

Authors+Show Affiliations

Pistón F
Instituto de Agricultura Sostenible, CSIC, Apdo. 4084, 14080 Córdoba, Spain.
Dorado G
No affiliation info available
Martín A
No affiliation info available
Barro F
No affiliation info available

MeSH

Amino Acid MotifsBase SequenceDNA PrimersDNA, ComplementaryGlutensHordeumHybridization, GeneticMolecular Sequence DataPlant ProteinsPolymerase Chain ReactionPolyploidyRNA, MessengerSequence AlignmentSequence Analysis, DNATime FactorsTranscription, Genetic

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14747917

Citation

Pistón, F, et al. "Cloning and Characterization of a Gamma-3 Hordein mRNA (cDNA) From Hordeum Chilense (Roem. Et Schult.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 108, no. 7, 2004, pp. 1359-65.
Pistón F, Dorado G, Martín A, et al. Cloning and characterization of a gamma-3 hordein mRNA (cDNA) from Hordeum chilense (Roem. et Schult.). Theor Appl Genet. 2004;108(7):1359-65.
Pistón, F., Dorado, G., Martín, A., & Barro, F. (2004). Cloning and characterization of a gamma-3 hordein mRNA (cDNA) from Hordeum chilense (Roem. et Schult.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 108(7), 1359-65.
Pistón F, et al. Cloning and Characterization of a Gamma-3 Hordein mRNA (cDNA) From Hordeum Chilense (Roem. Et Schult.). Theor Appl Genet. 2004;108(7):1359-65. PubMed PMID: 14747917.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and characterization of a gamma-3 hordein mRNA (cDNA) from Hordeum chilense (Roem. et Schult.). AU - Pistón,F, AU - Dorado,G, AU - Martín,A, AU - Barro,F, Y1 - 2004/01/28/ PY - 2003/07/22/received PY - 2003/11/14/accepted PY - 2004/1/30/pubmed PY - 2004/7/14/medline PY - 2004/1/30/entrez SP - 1359 EP - 65 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 108 IS - 7 N2 - Hordeum chilense is a wild relative of H. vulgare, cultivated barley, that has been successfully used in the synthesis of amphiploids by crossing with Triticum spp. These amphiploids-named generically x Tritordeum-have been tested under field conditions, and one of them, the hexaploid tritordeum obtained following chromosome doubling of the hybrid H. chilense x T. turgidum, shows traits of interest inherited from the barley parent. Of great interest is the allelic variation observed in the endosperm storage proteins and their influence on the breadmaking and malting quality of tritordeum. We report here two mRNA (cDNA) sequences for a gamma-3 hordein from two accession lines of H. chilense, H1 and H7, and their characterization by quantitative real time (QRT)-PCR in the developing endosperm. Sequences were obtained by rapid amplification of cDNA ends and "edge-to-edge" amplification of open reading frames from cDNA of H. chilense. Eight putative single nucleotide polymorphisms and one codon insertion were identified in the sequences of the H1 and H7 gamma-3 hordeins. The deduced amino acid sequences showed similar features to that of the gamma-3 hordein and gamma-gliadins from barley and wheat, respectively. While the repetitive motif (PQQQPF) is similar to that of the gamma-3 hordein from H. vulgare, there are 19 motif repeats in H. vulgare, whereas H. chilense shows 15 tandem repeats. The transcription of the genes encoding for the gamma-3 hordein were monitored by QRT-PCR: in both lines maximum transcription occurred 12 days after flowering. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/14747917/Cloning_and_characterization_of_a_gamma_3_hordein_mRNA__cDNA__from_Hordeum_chilense__Roem__et_Schult___ DB - PRIME DP - Unbound Medicine ER -