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Downregulation of follicle-stimulating hormone (FSH)-receptor messenger RNA levels in the hamster ovary: effect of the endogenous and exogenous FSH.
Biol Reprod. 2004 Jun; 70(6):1580-8.BR

Abstract

Although gonadotropins have been reported to downregulate FSH-receptor (FSHR) mRNA levels in the ovaries of female rats, the effect of the gonadotropin surge, particularly FSH, on hamster follicular FSHR mRNA levels warrants further examination. The objectives of the present study were to clone and determine the complete FSHR cDNA sequence of the hamster and to delineate the effects of endogenous and exogenous FSH on the steady-state levels of ovarian FSHR mRNA. Complete FSHR cDNA was derived from hamster ovarian total RNA by the strategy of 3'- and 5'-rapid amplification of cDNA ends. Ovaries were obtained before and after the endogenous gonadotropin surge or exogenous FSH administration, and the steady-state levels of FSHR mRNA were assessed by Northern blot hybridization. Cloned FSHR cDNA consists of a reading frame corresponding to exons 1-10 of the human FSHR gene and the 5'- and 3'-untranslated regions. The nucleic acid and amino acid sequences of the reading frame were at least 87% and 92% identical, respectively, to that of human, rat, and mouse FSHR. Furthermore, the amino acid sequence contained seven transmembrane domains characteristic of the FSHR. The steady-state levels of FSHR mRNA increased from estrus (Day 1) to reach a peak on proestrus (Day 4) noon; however, significant attenuation was noted following the gonadotropin surge, which was blocked by phenobarbital. Exogenous FSH also downregulated, both dose- and time-dependently, ovarian FSHR mRNA levels. These data indicate that the nucleic acid sequence of hamster FSHR has been identified and that FSH modulates FSHR mRNA levels in the hamster ovary.

Authors+Show Affiliations

Department of Physiology and Biophysics, University of Nebraska Medical Center, Omaha, 68198-4515, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14749302

Citation

Zhang, Yi-Ming, and Shyamal K. Roy. "Downregulation of Follicle-stimulating Hormone (FSH)-receptor Messenger RNA Levels in the Hamster Ovary: Effect of the Endogenous and Exogenous FSH." Biology of Reproduction, vol. 70, no. 6, 2004, pp. 1580-8.
Zhang YM, Roy SK. Downregulation of follicle-stimulating hormone (FSH)-receptor messenger RNA levels in the hamster ovary: effect of the endogenous and exogenous FSH. Biol Reprod. 2004;70(6):1580-8.
Zhang, Y. M., & Roy, S. K. (2004). Downregulation of follicle-stimulating hormone (FSH)-receptor messenger RNA levels in the hamster ovary: effect of the endogenous and exogenous FSH. Biology of Reproduction, 70(6), 1580-8.
Zhang YM, Roy SK. Downregulation of Follicle-stimulating Hormone (FSH)-receptor Messenger RNA Levels in the Hamster Ovary: Effect of the Endogenous and Exogenous FSH. Biol Reprod. 2004;70(6):1580-8. PubMed PMID: 14749302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Downregulation of follicle-stimulating hormone (FSH)-receptor messenger RNA levels in the hamster ovary: effect of the endogenous and exogenous FSH. AU - Zhang,Yi-Ming, AU - Roy,Shyamal K, Y1 - 2004/01/28/ PY - 2004/1/30/pubmed PY - 2004/12/16/medline PY - 2004/1/30/entrez SP - 1580 EP - 8 JF - Biology of reproduction JO - Biol Reprod VL - 70 IS - 6 N2 - Although gonadotropins have been reported to downregulate FSH-receptor (FSHR) mRNA levels in the ovaries of female rats, the effect of the gonadotropin surge, particularly FSH, on hamster follicular FSHR mRNA levels warrants further examination. The objectives of the present study were to clone and determine the complete FSHR cDNA sequence of the hamster and to delineate the effects of endogenous and exogenous FSH on the steady-state levels of ovarian FSHR mRNA. Complete FSHR cDNA was derived from hamster ovarian total RNA by the strategy of 3'- and 5'-rapid amplification of cDNA ends. Ovaries were obtained before and after the endogenous gonadotropin surge or exogenous FSH administration, and the steady-state levels of FSHR mRNA were assessed by Northern blot hybridization. Cloned FSHR cDNA consists of a reading frame corresponding to exons 1-10 of the human FSHR gene and the 5'- and 3'-untranslated regions. The nucleic acid and amino acid sequences of the reading frame were at least 87% and 92% identical, respectively, to that of human, rat, and mouse FSHR. Furthermore, the amino acid sequence contained seven transmembrane domains characteristic of the FSHR. The steady-state levels of FSHR mRNA increased from estrus (Day 1) to reach a peak on proestrus (Day 4) noon; however, significant attenuation was noted following the gonadotropin surge, which was blocked by phenobarbital. Exogenous FSH also downregulated, both dose- and time-dependently, ovarian FSHR mRNA levels. These data indicate that the nucleic acid sequence of hamster FSHR has been identified and that FSH modulates FSHR mRNA levels in the hamster ovary. SN - 0006-3363 UR - https://www.unboundmedicine.com/medline/citation/14749302/Downregulation_of_follicle_stimulating_hormone__FSH__receptor_messenger_RNA_levels_in_the_hamster_ovary:_effect_of_the_endogenous_and_exogenous_FSH_ L2 - https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.103.026898 DB - PRIME DP - Unbound Medicine ER -