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Substance P and enkephalinergic synapses onto neurokinin-1 receptor-immunoreactive neurons in the pre-Bötzinger complex of rats.
Eur J Neurosci. 2004 Jan; 19(1):65-75.EJ

Abstract

Our previous studies have demonstrated that neurokinin-1 receptor (NK1R)-immunoreactive (ir) neurons in the pre-Bötzinger Complex (pre-BötC), the hypothesized kernel of respiratory rhythmogenesis, receive both glutamatergic excitatory and GABAergic or glycinergic inhibitory inputs. Neuromodulators, such as substance P (SP) and opioids, play important roles in normal respiratory activity and respiratory disorders. The identification of the relationship between neurotransmitters and NK1R-ir neurons at the cellular level is essential for understanding the synaptic interaction within the pre-BötC network. Using immunofluorescence and immunogold-silver staining, we wished to exploit SP and enkephalin (ENK) immunoreactivity and their relationships with glutamate, GABA, glycine, or NK1R in the pre-BötC in adult Sprague-Dawley rats. The pre-BötC contained a substantial amount of SP-ir and ENK-ir boutons. They were largely colocalized with glutamate and much less so with GABA. Glycine immunoreactivity was rarely found in either SP-ir or ENK-ir boutons. A number of SP-ir boutons were ENK-ir as well. Synapses were commonly found between SP-ir or ENK-ir terminals and NK1R-ir neurons in the pre-BötC. Most of them were asymmetric. Symmetric synapses made up 10% of all synapses examined between SP-ir boutons and NK1R-ir neurons, and 19% of ENK/NK1R synapses. Colocalization of SP and/or ENK with glutamate in boutons in the pre-BötC implies the combined synaptic release of excitatory amino acid and neuropeptides, which may exert combined post-synaptic effects onto NK1R-ir neurons and contribute to respiratory activity.

Authors+Show Affiliations

Institute of Neurosciences, The Fourth Military Medical University, 17 Chang Le Xi Road, Xi'an 710032, PR, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14750964

Citation

Liu, Ying-Ying, et al. "Substance P and Enkephalinergic Synapses Onto Neurokinin-1 Receptor-immunoreactive Neurons in the pre-Bötzinger Complex of Rats." The European Journal of Neuroscience, vol. 19, no. 1, 2004, pp. 65-75.
Liu YY, Wong-Riley MT, Liu JP, et al. Substance P and enkephalinergic synapses onto neurokinin-1 receptor-immunoreactive neurons in the pre-Bötzinger complex of rats. Eur J Neurosci. 2004;19(1):65-75.
Liu, Y. Y., Wong-Riley, M. T., Liu, J. P., Wei, X. Y., Jia, Y., Liu, H. L., Fujiyama, F., & Ju, G. (2004). Substance P and enkephalinergic synapses onto neurokinin-1 receptor-immunoreactive neurons in the pre-Bötzinger complex of rats. The European Journal of Neuroscience, 19(1), 65-75.
Liu YY, et al. Substance P and Enkephalinergic Synapses Onto Neurokinin-1 Receptor-immunoreactive Neurons in the pre-Bötzinger Complex of Rats. Eur J Neurosci. 2004;19(1):65-75. PubMed PMID: 14750964.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Substance P and enkephalinergic synapses onto neurokinin-1 receptor-immunoreactive neurons in the pre-Bötzinger complex of rats. AU - Liu,Ying-Ying, AU - Wong-Riley,Margaret T T, AU - Liu,Jin-Ping, AU - Wei,Xiao-Yan, AU - Jia,Yi, AU - Liu,Hui-Ling, AU - Fujiyama,Fumino, AU - Ju,Gong, PY - 2004/1/31/pubmed PY - 2004/6/21/medline PY - 2004/1/31/entrez SP - 65 EP - 75 JF - The European journal of neuroscience JO - Eur. J. Neurosci. VL - 19 IS - 1 N2 - Our previous studies have demonstrated that neurokinin-1 receptor (NK1R)-immunoreactive (ir) neurons in the pre-Bötzinger Complex (pre-BötC), the hypothesized kernel of respiratory rhythmogenesis, receive both glutamatergic excitatory and GABAergic or glycinergic inhibitory inputs. Neuromodulators, such as substance P (SP) and opioids, play important roles in normal respiratory activity and respiratory disorders. The identification of the relationship between neurotransmitters and NK1R-ir neurons at the cellular level is essential for understanding the synaptic interaction within the pre-BötC network. Using immunofluorescence and immunogold-silver staining, we wished to exploit SP and enkephalin (ENK) immunoreactivity and their relationships with glutamate, GABA, glycine, or NK1R in the pre-BötC in adult Sprague-Dawley rats. The pre-BötC contained a substantial amount of SP-ir and ENK-ir boutons. They were largely colocalized with glutamate and much less so with GABA. Glycine immunoreactivity was rarely found in either SP-ir or ENK-ir boutons. A number of SP-ir boutons were ENK-ir as well. Synapses were commonly found between SP-ir or ENK-ir terminals and NK1R-ir neurons in the pre-BötC. Most of them were asymmetric. Symmetric synapses made up 10% of all synapses examined between SP-ir boutons and NK1R-ir neurons, and 19% of ENK/NK1R synapses. Colocalization of SP and/or ENK with glutamate in boutons in the pre-BötC implies the combined synaptic release of excitatory amino acid and neuropeptides, which may exert combined post-synaptic effects onto NK1R-ir neurons and contribute to respiratory activity. SN - 0953-816X UR - https://www.unboundmedicine.com/medline/citation/14750964/Substance_P_and_enkephalinergic_synapses_onto_neurokinin_1_receptor_immunoreactive_neurons_in_the_pre_Bötzinger_complex_of_rats_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0953-816X&date=2004&volume=19&issue=1&spage=65 DB - PRIME DP - Unbound Medicine ER -