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Simultaneous analysis of hepatotoxic pyrrolizidine alkaloids and N-oxides in comfrey root by LC-ion trap mass spectrometry.
Analyst 2004; 129(2):150-6A

Abstract

The purpose of the current study was to develop a LC-MS(n) method for the analysis of pyrrolizidine alkaloids (PAs) in comfrey. Published data presents an extensive list of PAs and their N-oxides present in comfrey. However, standards are not commercially available for any of the PAs typically present in comfrey. Those PAs that are not stereoisomers were readily resolved on a C(18) column using a water-acetonitrile gradient as the mobile phase. The use of a selective technique, LC-MS/MS, allowed us to identify groups of PAs and their N-oxides, as well as identify the number of PAs present in each group, including those that were not completely resolved chromatographically.

Authors+Show Affiliations

US Food and Drug Administration, Forensic Chemistry Center, Cincinnati, Ohio 45237-3097, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14752559

Citation

Wuilloud, Jorgelina C A., et al. "Simultaneous Analysis of Hepatotoxic Pyrrolizidine Alkaloids and N-oxides in Comfrey Root By LC-ion Trap Mass Spectrometry." The Analyst, vol. 129, no. 2, 2004, pp. 150-6.
Wuilloud JC, Gratze SR, Gamble BM, et al. Simultaneous analysis of hepatotoxic pyrrolizidine alkaloids and N-oxides in comfrey root by LC-ion trap mass spectrometry. Analyst. 2004;129(2):150-6.
Wuilloud, J. C., Gratze, S. R., Gamble, B. M., & Wolnik, K. A. (2004). Simultaneous analysis of hepatotoxic pyrrolizidine alkaloids and N-oxides in comfrey root by LC-ion trap mass spectrometry. The Analyst, 129(2), pp. 150-6.
Wuilloud JC, et al. Simultaneous Analysis of Hepatotoxic Pyrrolizidine Alkaloids and N-oxides in Comfrey Root By LC-ion Trap Mass Spectrometry. Analyst. 2004;129(2):150-6. PubMed PMID: 14752559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous analysis of hepatotoxic pyrrolizidine alkaloids and N-oxides in comfrey root by LC-ion trap mass spectrometry. AU - Wuilloud,Jorgelina C A, AU - Gratze,Samuel R, AU - Gamble,Bryan M, AU - Wolnik,Karen A, Y1 - 2004/01/08/ PY - 2004/1/31/pubmed PY - 2004/5/22/medline PY - 2004/1/31/entrez SP - 150 EP - 6 JF - The Analyst JO - Analyst VL - 129 IS - 2 N2 - The purpose of the current study was to develop a LC-MS(n) method for the analysis of pyrrolizidine alkaloids (PAs) in comfrey. Published data presents an extensive list of PAs and their N-oxides present in comfrey. However, standards are not commercially available for any of the PAs typically present in comfrey. Those PAs that are not stereoisomers were readily resolved on a C(18) column using a water-acetonitrile gradient as the mobile phase. The use of a selective technique, LC-MS/MS, allowed us to identify groups of PAs and their N-oxides, as well as identify the number of PAs present in each group, including those that were not completely resolved chromatographically. SN - 0003-2654 UR - https://www.unboundmedicine.com/medline/citation/14752559/Simultaneous_analysis_of_hepatotoxic_pyrrolizidine_alkaloids_and_N_oxides_in_comfrey_root_by_LC_ion_trap_mass_spectrometry_ L2 - https://doi.org/10.1039/b311030c DB - PRIME DP - Unbound Medicine ER -