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The use of reconstructed human skin to evaluate UV-induced modifications and sunscreen efficacy.
Exp Dermatol. 2003; 12 Suppl 2:64-70.ED

Abstract

Biological and clinical effects of sun exposures are characterized by short-term reactions, i.e. sunburn reaction and suntan, as well as long-term consequences corresponding to photoaging and photocancers. We have developed several human in vitro three-dimensional models in order to assess both the photodamage and the photoprotection afforded by sunscreens. Using a full thickness reconstructed skin comprising a differentiated epidermis and a living dermal equivalent, UVB- and UVA-induced biological markers could be found at both the keratinocyte and the fibroblast level. Typical markers of the sunburn reaction could be reproduced in that model as well as dermal damages related to the photoaging process. Another model of reconstructed epidermis, comprising keratinocytes but also melanocytes and Langerhans cells, has been developed. The study of the UV-induced pigmentation as possible using the pigmented reconstructed epidermis and allowing to reproduce the epidermal melanin unit. The assessment of cellular parameters related to UV-induced immunosuppression could be performed using the reconstructed epidermis containing Langerhans cells. Exposure to solar-simulated radiation provokes morphological alterations and the reduction in numbers of Langerhans cells within the exposed epidermis. Using all these models, the efficiency of sunscreens could be envisaged after topical application. The results showed that appropriate sunscreens could efficiently prevent the damage described above.

Authors+Show Affiliations

Life Sciences Research, L'Oréal, Centre Charles Zviak, Clichy, France. cduval@recherche.loreal.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14756526

Citation

Duval, Christine, et al. "The Use of Reconstructed Human Skin to Evaluate UV-induced Modifications and Sunscreen Efficacy." Experimental Dermatology, vol. 12 Suppl 2, 2003, pp. 64-70.
Duval C, Schmidt R, Regnier M, et al. The use of reconstructed human skin to evaluate UV-induced modifications and sunscreen efficacy. Exp Dermatol. 2003;12 Suppl 2:64-70.
Duval, C., Schmidt, R., Regnier, M., Facy, V., Asselineau, D., & Bernerd, F. (2003). The use of reconstructed human skin to evaluate UV-induced modifications and sunscreen efficacy. Experimental Dermatology, 12 Suppl 2, 64-70.
Duval C, et al. The Use of Reconstructed Human Skin to Evaluate UV-induced Modifications and Sunscreen Efficacy. Exp Dermatol. 2003;12 Suppl 2:64-70. PubMed PMID: 14756526.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The use of reconstructed human skin to evaluate UV-induced modifications and sunscreen efficacy. AU - Duval,Christine, AU - Schmidt,Rainer, AU - Regnier,Marcelle, AU - Facy,Valérie, AU - Asselineau,Daniel, AU - Bernerd,Françoise, PY - 2004/2/6/pubmed PY - 2004/7/9/medline PY - 2004/2/6/entrez SP - 64 EP - 70 JF - Experimental dermatology JO - Exp Dermatol VL - 12 Suppl 2 N2 - Biological and clinical effects of sun exposures are characterized by short-term reactions, i.e. sunburn reaction and suntan, as well as long-term consequences corresponding to photoaging and photocancers. We have developed several human in vitro three-dimensional models in order to assess both the photodamage and the photoprotection afforded by sunscreens. Using a full thickness reconstructed skin comprising a differentiated epidermis and a living dermal equivalent, UVB- and UVA-induced biological markers could be found at both the keratinocyte and the fibroblast level. Typical markers of the sunburn reaction could be reproduced in that model as well as dermal damages related to the photoaging process. Another model of reconstructed epidermis, comprising keratinocytes but also melanocytes and Langerhans cells, has been developed. The study of the UV-induced pigmentation as possible using the pigmented reconstructed epidermis and allowing to reproduce the epidermal melanin unit. The assessment of cellular parameters related to UV-induced immunosuppression could be performed using the reconstructed epidermis containing Langerhans cells. Exposure to solar-simulated radiation provokes morphological alterations and the reduction in numbers of Langerhans cells within the exposed epidermis. Using all these models, the efficiency of sunscreens could be envisaged after topical application. The results showed that appropriate sunscreens could efficiently prevent the damage described above. SN - 0906-6705 UR - https://www.unboundmedicine.com/medline/citation/14756526/The_use_of_reconstructed_human_skin_to_evaluate_UV_induced_modifications_and_sunscreen_efficacy_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0906-6705&date=2003&volume=12&issue=&spage=64 DB - PRIME DP - Unbound Medicine ER -