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Microglia/macrophage-specific protein Iba1 binds to fimbrin and enhances its actin-bundling activity.
J Neurochem 2004; 88(4):844-56JN

Abstract

Ionized calcium binding adaptor molecule 1 (Iba1) is a microglia/macrophage-specific calcium-binding protein. Iba1 has the actin-bundling activity and participates in membrane ruffling and phagocytosis in activated microglia. In order to understand the Iba1-related intracellular signalling pathway in greater detail, we employed a yeast two-hybrid screen to isolate an Iba1-interacting molecule and identified another actin-bundling protein, L-fimbrin. In response to stimulation, L-fimbrin accumulated and co-localized with Iba1 in membrane ruffles induced by M-CSF-stimulation and phagocytic cups formed by IgG-opsonized beads in microglial cell line MG5. L-fimbrin was shown to associate with Iba1 in cell lysate of COS-7 expressing L-fimbrin and Iba1. By using purified proteins, direct binding of Iba1 to L-fimbrin was demonstrated by immunoprecipitation, glutathione S-transferase pull-down assays and ligand overlay assays. The binding of Iba1 was also found to increase the actin-bundling activity of L-fimbrin. These results indicate that Iba1 forms complexes with L-fimbrin in membrane ruffles and phagocytic cups, and suggest that Iba1 co-operates with L-fimbrin in modulating actin reorganization to facilitate cell migration and phagocytosis by microglia.

Authors+Show Affiliations

Department of Neurochemistry, National Institute of Neuroscience, Tokyo, Japan. kohsaka@ncnp.go.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14756805

Citation

Ohsawa, Keiko, et al. "Microglia/macrophage-specific Protein Iba1 Binds to Fimbrin and Enhances Its Actin-bundling Activity." Journal of Neurochemistry, vol. 88, no. 4, 2004, pp. 844-56.
Ohsawa K, Imai Y, Sasaki Y, et al. Microglia/macrophage-specific protein Iba1 binds to fimbrin and enhances its actin-bundling activity. J Neurochem. 2004;88(4):844-56.
Ohsawa, K., Imai, Y., Sasaki, Y., & Kohsaka, S. (2004). Microglia/macrophage-specific protein Iba1 binds to fimbrin and enhances its actin-bundling activity. Journal of Neurochemistry, 88(4), pp. 844-56.
Ohsawa K, et al. Microglia/macrophage-specific Protein Iba1 Binds to Fimbrin and Enhances Its Actin-bundling Activity. J Neurochem. 2004;88(4):844-56. PubMed PMID: 14756805.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microglia/macrophage-specific protein Iba1 binds to fimbrin and enhances its actin-bundling activity. AU - Ohsawa,Keiko, AU - Imai,Yoshinori, AU - Sasaki,Yo, AU - Kohsaka,Shinichi, PY - 2004/2/6/pubmed PY - 2004/3/17/medline PY - 2004/2/6/entrez SP - 844 EP - 56 JF - Journal of neurochemistry JO - J. Neurochem. VL - 88 IS - 4 N2 - Ionized calcium binding adaptor molecule 1 (Iba1) is a microglia/macrophage-specific calcium-binding protein. Iba1 has the actin-bundling activity and participates in membrane ruffling and phagocytosis in activated microglia. In order to understand the Iba1-related intracellular signalling pathway in greater detail, we employed a yeast two-hybrid screen to isolate an Iba1-interacting molecule and identified another actin-bundling protein, L-fimbrin. In response to stimulation, L-fimbrin accumulated and co-localized with Iba1 in membrane ruffles induced by M-CSF-stimulation and phagocytic cups formed by IgG-opsonized beads in microglial cell line MG5. L-fimbrin was shown to associate with Iba1 in cell lysate of COS-7 expressing L-fimbrin and Iba1. By using purified proteins, direct binding of Iba1 to L-fimbrin was demonstrated by immunoprecipitation, glutathione S-transferase pull-down assays and ligand overlay assays. The binding of Iba1 was also found to increase the actin-bundling activity of L-fimbrin. These results indicate that Iba1 forms complexes with L-fimbrin in membrane ruffles and phagocytic cups, and suggest that Iba1 co-operates with L-fimbrin in modulating actin reorganization to facilitate cell migration and phagocytosis by microglia. SN - 0022-3042 UR - https://www.unboundmedicine.com/medline/citation/14756805/Microglia/macrophage_specific_protein_Iba1_binds_to_fimbrin_and_enhances_its_actin_bundling_activity_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0022-3042&date=2004&volume=88&issue=4&spage=844 DB - PRIME DP - Unbound Medicine ER -