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[Analysis of 187 children with enteroviral central nervous system infection in Shandong area].
Zhonghua Er Ke Za Zhi. 2003 Mar; 41(3):199-202.ZE

Abstract

OBJECTIVE

To evaluate the diagnostic potential of previously published enterovirus (EV) reverse transcription polymerase chain reaction (RT-PCR) assay in detection of EV in CSF samples from children with a diagnosis of aseptic meningitis and to investigate the clinical characteristics of the patients seen in Shandong.

METHODS

EV RNA was detected in 187 CSF samples and serum and/or urine samples of a part of patients by RT-PCR and viral culture technique.

RESULTS

RT-PCR was positive in all 62 CSF specimens which were positive by cell culture (100%). In addition, 93 of 125 (74.4%) CSF samples negative by cell culture were RT-PCR positive. In 4 of these 93 (4.3%) patients, viral culture of specimens from other sites (serum or urine) was also positive. The sensitivity of CSF RT-PCR based on clinical diagnosis in patients with meningitis of negative bacterial culture results was 82.9% (155/187), which was considerably higher than the sensitivity of CSF virus culture 33.2% (62/187). The results of RT-PCR can be reported within 4 hours, whereas the viral culture of CSF requires 4.6 days for a cytopathic effect to develop. EV meningitis occurred in a sporadic form and in some areas there were outbreaks. The clinical characteristics of 155 patients with EV meningitis were different in different age groups.

CONCLUSION

EV was one of the most common causes of aseptic meningitis in Shandong area. The RT-PCR assay was rapid, sensitive and specific for the diagnosis of EV meningitis and may be a potential tests to shorten hospital stay and reduce the use of antibiotics.

Authors+Show Affiliations

Department of Pediatrics, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003 China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

14756959

Citation

Chen, Zong-bo, et al. "[Analysis of 187 Children With Enteroviral Central Nervous System Infection in Shandong Area]." Zhonghua Er Ke Za Zhi = Chinese Journal of Pediatrics, vol. 41, no. 3, 2003, pp. 199-202.
Chen ZB, Fan XW, Dong YS, et al. [Analysis of 187 children with enteroviral central nervous system infection in Shandong area]. Zhonghua Er Ke Za Zhi. 2003;41(3):199-202.
Chen, Z. B., Fan, X. W., Dong, Y. S., Sun, J. Q., & Liu, Y. C. (2003). [Analysis of 187 children with enteroviral central nervous system infection in Shandong area]. Zhonghua Er Ke Za Zhi = Chinese Journal of Pediatrics, 41(3), 199-202.
Chen ZB, et al. [Analysis of 187 Children With Enteroviral Central Nervous System Infection in Shandong Area]. Zhonghua Er Ke Za Zhi. 2003;41(3):199-202. PubMed PMID: 14756959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Analysis of 187 children with enteroviral central nervous system infection in Shandong area]. AU - Chen,Zong-bo, AU - Fan,Xi-wen, AU - Dong,Yong-sui, AU - Sun,Jin-qiao, AU - Liu,Yuan-chang, PY - 2004/2/6/pubmed PY - 2004/4/23/medline PY - 2004/2/6/entrez SP - 199 EP - 202 JF - Zhonghua er ke za zhi = Chinese journal of pediatrics JO - Zhonghua Er Ke Za Zhi VL - 41 IS - 3 N2 - OBJECTIVE: To evaluate the diagnostic potential of previously published enterovirus (EV) reverse transcription polymerase chain reaction (RT-PCR) assay in detection of EV in CSF samples from children with a diagnosis of aseptic meningitis and to investigate the clinical characteristics of the patients seen in Shandong. METHODS: EV RNA was detected in 187 CSF samples and serum and/or urine samples of a part of patients by RT-PCR and viral culture technique. RESULTS: RT-PCR was positive in all 62 CSF specimens which were positive by cell culture (100%). In addition, 93 of 125 (74.4%) CSF samples negative by cell culture were RT-PCR positive. In 4 of these 93 (4.3%) patients, viral culture of specimens from other sites (serum or urine) was also positive. The sensitivity of CSF RT-PCR based on clinical diagnosis in patients with meningitis of negative bacterial culture results was 82.9% (155/187), which was considerably higher than the sensitivity of CSF virus culture 33.2% (62/187). The results of RT-PCR can be reported within 4 hours, whereas the viral culture of CSF requires 4.6 days for a cytopathic effect to develop. EV meningitis occurred in a sporadic form and in some areas there were outbreaks. The clinical characteristics of 155 patients with EV meningitis were different in different age groups. CONCLUSION: EV was one of the most common causes of aseptic meningitis in Shandong area. The RT-PCR assay was rapid, sensitive and specific for the diagnosis of EV meningitis and may be a potential tests to shorten hospital stay and reduce the use of antibiotics. SN - 0578-1310 UR - https://www.unboundmedicine.com/medline/citation/14756959/[Analysis_of_187_children_with_enteroviral_central_nervous_system_infection_in_Shandong_area]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0578-1310&year=2003&vol=41&issue=3&fpage=199 DB - PRIME DP - Unbound Medicine ER -