Tags

Type your tag names separated by a space and hit enter

Microcystin production by Radiocystis fernandoi (Chroococcales, Cyanobacteria) isolated from a drinking water reservoir in the city of Belém, PA, Brazilian Amazonia region.
Toxicon 2003; 42(7):709-13T

Abstract

During the monitoring of toxic cyanobacteria in the Utinga Reservoir, which is the main drinking water supply for the city of Belém, PA, Brazil, a Radiocystis fernandoi strain (SPC714) was isolated. This non-axenic strain was submitted to a toxicity bioassay with mice and microcystin production analyzed by HPLC-DAD. The species was identified based on cultured and natural preserved material. Morphometric, developmental and reproductive characteristics were analyzed. The strain was cultured in liquid ASM-1 medium, at 25+/-1 degrees C, at an incident irradiance of 20 micromol photon m(-2)s(-1) and constant aeration. At the end of the exponential growth phase, cells were lyophilized and submitted to toxicity tests. The strain showed high toxicity to mice, by intraperitoneal route, with an approximate LD100 of 60 mg kg(-1) of body weight, producing characteristic symptoms of hepatotoxicity. Analyses performed by HPLC-DAD confirmed the production of microcystins, in a concentration of 3.83 microg mg(-1) of lyophilized cells. This is the first reference related to the toxicity of the genus Radiocystis.

Authors+Show Affiliations

Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Pará, 66075-110 Belém, PA, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14757200

Citation

dos S Vieira, José Maria, et al. "Microcystin Production By Radiocystis Fernandoi (Chroococcales, Cyanobacteria) Isolated From a Drinking Water Reservoir in the City of Belém, PA, Brazilian Amazonia Region." Toxicon : Official Journal of the International Society On Toxinology, vol. 42, no. 7, 2003, pp. 709-13.
dos S Vieira JM, de P Azevedo MT, de Oliveira Azevedo SM, et al. Microcystin production by Radiocystis fernandoi (Chroococcales, Cyanobacteria) isolated from a drinking water reservoir in the city of Belém, PA, Brazilian Amazonia region. Toxicon. 2003;42(7):709-13.
dos S Vieira, J. M., de P Azevedo, M. T., de Oliveira Azevedo, S. M., Honda, R. Y., & Corrêa, B. (2003). Microcystin production by Radiocystis fernandoi (Chroococcales, Cyanobacteria) isolated from a drinking water reservoir in the city of Belém, PA, Brazilian Amazonia region. Toxicon : Official Journal of the International Society On Toxinology, 42(7), pp. 709-13.
dos S Vieira JM, et al. Microcystin Production By Radiocystis Fernandoi (Chroococcales, Cyanobacteria) Isolated From a Drinking Water Reservoir in the City of Belém, PA, Brazilian Amazonia Region. Toxicon. 2003;42(7):709-13. PubMed PMID: 14757200.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microcystin production by Radiocystis fernandoi (Chroococcales, Cyanobacteria) isolated from a drinking water reservoir in the city of Belém, PA, Brazilian Amazonia region. AU - dos S Vieira,José Maria, AU - de P Azevedo,Maria Teresa, AU - de Oliveira Azevedo,Sandra Maria Feliciano, AU - Honda,Ricardo Yukio, AU - Corrêa,Benedito, PY - 2004/2/6/pubmed PY - 2004/3/5/medline PY - 2004/2/6/entrez SP - 709 EP - 13 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 42 IS - 7 N2 - During the monitoring of toxic cyanobacteria in the Utinga Reservoir, which is the main drinking water supply for the city of Belém, PA, Brazil, a Radiocystis fernandoi strain (SPC714) was isolated. This non-axenic strain was submitted to a toxicity bioassay with mice and microcystin production analyzed by HPLC-DAD. The species was identified based on cultured and natural preserved material. Morphometric, developmental and reproductive characteristics were analyzed. The strain was cultured in liquid ASM-1 medium, at 25+/-1 degrees C, at an incident irradiance of 20 micromol photon m(-2)s(-1) and constant aeration. At the end of the exponential growth phase, cells were lyophilized and submitted to toxicity tests. The strain showed high toxicity to mice, by intraperitoneal route, with an approximate LD100 of 60 mg kg(-1) of body weight, producing characteristic symptoms of hepatotoxicity. Analyses performed by HPLC-DAD confirmed the production of microcystins, in a concentration of 3.83 microg mg(-1) of lyophilized cells. This is the first reference related to the toxicity of the genus Radiocystis. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/14757200/abstract/Microcystin_production_by_Radiocystis_fernandoi__Chroococcales_Cyanobacteria__isolated_from_a_drinking_water_reservoir_in_the_city_of_Belém_PA_Brazilian_Amazonia_region_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S004101010300240X DB - PRIME DP - Unbound Medicine ER -