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Glutathione peroxidase induction protects Saccharomyces cerevisiae sod1deltasod2delta double mutants against oxidative damage.
Braz J Med Biol Res. 2004 Feb; 37(2):159-65.BJ

Abstract

Saccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1delta, sod2delta and the double mutant) were subjected to H2O2 stress in the stationary phase. The highest sensitivity was observed in the sod2delta mutant, while the sod1deltasod2delta double mutant was not sensitive. Sod mutants had lower catalase activity (44%) than wild-type cells, independent of H2O2 stress. Untreated cells of sod1deltasod2delta double mutants showed increased glutathione peroxidase activity (126%), while sod1delta had lower activity (77%) than the wild type. Glutathione levels in sod1delta were increased (200-260%) after exposure to various H2O2 concentrations. In addition, the highest malondialdehyde levels could be observed without H2O2 treatment in sod1delta (167%) and sod2delta (225%) mutants. In contrast, the level of malondialdehyde in the sod1deltasod2delta double mutant was indistinguishable from that of the wild type. These results suggest that resistance to H2O2 by sod1deltasod2delta cells depends on the induction of glutathione peroxidase and is independent of catalase, and that glutathione is a primary antioxidant in the defense against H2O2 in stationary phase sod1delta mutants.

Authors+Show Affiliations

Laboratório de Estresse Oxidativo, Departamento de Biofísica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14762569

Citation

Manfredini, V, et al. "Glutathione Peroxidase Induction Protects Saccharomyces Cerevisiae Sod1deltasod2delta Double Mutants Against Oxidative Damage." Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas, vol. 37, no. 2, 2004, pp. 159-65.
Manfredini V, Roehrs R, Peralba MC, et al. Glutathione peroxidase induction protects Saccharomyces cerevisiae sod1deltasod2delta double mutants against oxidative damage. Braz J Med Biol Res. 2004;37(2):159-65.
Manfredini, V., Roehrs, R., Peralba, M. C., Henriques, J. A., Saffi, J., Ramos, A. L., & Benfato, M. S. (2004). Glutathione peroxidase induction protects Saccharomyces cerevisiae sod1deltasod2delta double mutants against oxidative damage. Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas, 37(2), 159-65.
Manfredini V, et al. Glutathione Peroxidase Induction Protects Saccharomyces Cerevisiae Sod1deltasod2delta Double Mutants Against Oxidative Damage. Braz J Med Biol Res. 2004;37(2):159-65. PubMed PMID: 14762569.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glutathione peroxidase induction protects Saccharomyces cerevisiae sod1deltasod2delta double mutants against oxidative damage. AU - Manfredini,V, AU - Roehrs,R, AU - Peralba,M C R, AU - Henriques,J A P, AU - Saffi,J, AU - Ramos,A L L P, AU - Benfato,M S, Y1 - 2004/01/30/ PY - 2004/2/6/pubmed PY - 2004/7/20/medline PY - 2004/2/6/entrez SP - 159 EP - 65 JF - Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas JO - Braz J Med Biol Res VL - 37 IS - 2 N2 - Saccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1delta, sod2delta and the double mutant) were subjected to H2O2 stress in the stationary phase. The highest sensitivity was observed in the sod2delta mutant, while the sod1deltasod2delta double mutant was not sensitive. Sod mutants had lower catalase activity (44%) than wild-type cells, independent of H2O2 stress. Untreated cells of sod1deltasod2delta double mutants showed increased glutathione peroxidase activity (126%), while sod1delta had lower activity (77%) than the wild type. Glutathione levels in sod1delta were increased (200-260%) after exposure to various H2O2 concentrations. In addition, the highest malondialdehyde levels could be observed without H2O2 treatment in sod1delta (167%) and sod2delta (225%) mutants. In contrast, the level of malondialdehyde in the sod1deltasod2delta double mutant was indistinguishable from that of the wild type. These results suggest that resistance to H2O2 by sod1deltasod2delta cells depends on the induction of glutathione peroxidase and is independent of catalase, and that glutathione is a primary antioxidant in the defense against H2O2 in stationary phase sod1delta mutants. SN - 0100-879X UR - https://www.unboundmedicine.com/medline/citation/14762569/Glutathione_peroxidase_induction_protects_Saccharomyces_cerevisiae_sod1deltasod2delta_double_mutants_against_oxidative_damage_ L2 - https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200001&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -