Tags

Type your tag names separated by a space and hit enter

Suitability of N,O-bis(trimethylsilyl)trifluoroacetamide and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide as derivatization reagents for the determination of the estrogens estrone and 17alpha-ethinylestradiol by gas chromatography-mass spectrometry.
J Chromatogr A. 2004 Feb 13; 1026(1-2):295-300.JC

Abstract

This paper describes apreviously unreported problem with the use of N,O-bis-(trimethylsilyl)trifluoroacetamide (BSTFA) and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) to derivatise the natural hormone estrone (E1) and the synthetic estrogen 17alpha-ethinylestradiol (EE2). The resulting trimethylsilyl (TMS) and t-butyldimethylsilyl (TBS) derivatives of EE2 were partially converted to their respective El derivatives. Therefore, these reagents may not be suitable for simultaneous determination of estrogens in environmental samples, which raises questions about the reliability of results from some earlier studies.

Authors+Show Affiliations

Shareef A
Colloid and Environmental Chemistry Laboratory, La Trobe University, PO Box 199, Bendigo, Vic. 3552, Australia. a.shareef@bendigo.latrobe.edu.au
Parnis CJ
No affiliation info available
Angove MJ
No affiliation info available
Wells JD
No affiliation info available
Johnson BB
No affiliation info available

MeSH

AcetamidesEnvironmentEstroneFluoroacetatesGas Chromatography-Mass SpectrometryMolecular StructureNorethynodrelOrganosilicon CompoundsReproducibility of ResultsTrifluoroacetic AcidTrimethylsilyl Compounds

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14763757

Citation

Shareef, Ali, et al. "Suitability of N,O-bis(trimethylsilyl)trifluoroacetamide and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide as Derivatization Reagents for the Determination of the Estrogens Estrone and 17alpha-ethinylestradiol By Gas Chromatography-mass Spectrometry." Journal of Chromatography. A, vol. 1026, no. 1-2, 2004, pp. 295-300.
Shareef A, Parnis CJ, Angove MJ, et al. Suitability of N,O-bis(trimethylsilyl)trifluoroacetamide and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide as derivatization reagents for the determination of the estrogens estrone and 17alpha-ethinylestradiol by gas chromatography-mass spectrometry. J Chromatogr A. 2004;1026(1-2):295-300.
Shareef, A., Parnis, C. J., Angove, M. J., Wells, J. D., & Johnson, B. B. (2004). Suitability of N,O-bis(trimethylsilyl)trifluoroacetamide and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide as derivatization reagents for the determination of the estrogens estrone and 17alpha-ethinylestradiol by gas chromatography-mass spectrometry. Journal of Chromatography. A, 1026(1-2), 295-300.
Shareef A, et al. Suitability of N,O-bis(trimethylsilyl)trifluoroacetamide and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide as Derivatization Reagents for the Determination of the Estrogens Estrone and 17alpha-ethinylestradiol By Gas Chromatography-mass Spectrometry. J Chromatogr A. 2004 Feb 13;1026(1-2):295-300. PubMed PMID: 14763757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suitability of N,O-bis(trimethylsilyl)trifluoroacetamide and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide as derivatization reagents for the determination of the estrogens estrone and 17alpha-ethinylestradiol by gas chromatography-mass spectrometry. AU - Shareef,Ali, AU - Parnis,Craig J, AU - Angove,Michael J, AU - Wells,John D, AU - Johnson,Bruce B, PY - 2004/2/7/pubmed PY - 2004/12/16/medline PY - 2004/2/7/entrez SP - 295 EP - 300 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1026 IS - 1-2 N2 - This paper describes apreviously unreported problem with the use of N,O-bis-(trimethylsilyl)trifluoroacetamide (BSTFA) and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) to derivatise the natural hormone estrone (E1) and the synthetic estrogen 17alpha-ethinylestradiol (EE2). The resulting trimethylsilyl (TMS) and t-butyldimethylsilyl (TBS) derivatives of EE2 were partially converted to their respective El derivatives. Therefore, these reagents may not be suitable for simultaneous determination of estrogens in environmental samples, which raises questions about the reliability of results from some earlier studies. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/14763757/Suitability_of_NO_bis_trimethylsilyl_trifluoroacetamide_and_N__tert_butyldimethylsilyl__N_methyltrifluoroacetamide_as_derivatization_reagents_for_the_determination_of_the_estrogens_estrone_and_17alpha_ethinylestradiol_by_gas_chromatography_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓2]

Related Citations

More