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Transient expression of GABAA receptor alpha2 and alpha3 subunits in differentiating cerebellar neurons.
Brain Res Dev Brain Res 2004; 148(2):169-77BR

Abstract

In the adult mammalian brain, synaptic transmission mediated by gamma-amino butyric acid (GABA) plays a role in inhibition of excitatory synaptic transmission. During brain development, GABA is involved in brain morphogenesis. To clarify how GABA exerts its effect on immature neurons, we examined the expression of the GABAA receptor alpha2 and alpha3 subunits, which are abundantly expressed before alpha1 and alpha6 subunits appear, in the developing mouse cerebellum using in situ hybridization. Proliferating neuronal precursors in the ventricular zone and external granular layer expressed neither alpha2 nor alpha3 subunits. Hybridization signals for the alpha2 and alpha3 subunit mRNAs first appeared in the differentiating zone at embryonic day 13 (E13). The alpha2 subunit was detected in the migrating and differentiating granule cells and cerebellar nucleus neurons until postnatal day 14 (P14). Hybridization signals for the alpha3 subunit mRNA, on the other hand, were localized in the developing Purkinje cells and cerebellar nucleus neurons, and disappeared from Purkinje cells by the end of first postnatal week. Taken together, this indicated that the alpha2 and alpha3 subunits were abundantly expressed in distinct types of cerebellar neurons after completing cell proliferation while forming the neural network. These results suggest that GABA might extrasynaptically activate the GABAA receptors containing alpha2 and/or alpha3 subunits on the differentiating neurons before finishing the formation of synapses and networks, and could be involved in neuronal differentiation and maturation in the cerebellum.

Authors+Show Affiliations

Department of Molecular Neuroanatomy, Hokkaido University School of Medicine, Kita-15 Nishi-7, Kita-Ku, Sapporo 060-8638, Japan. takachan@med.hokudai.ac.jpNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14766194

Citation

Takayama, Chitoshi, and Yoshiro Inoue. "Transient Expression of GABAA Receptor Alpha2 and Alpha3 Subunits in Differentiating Cerebellar Neurons." Brain Research. Developmental Brain Research, vol. 148, no. 2, 2004, pp. 169-77.
Takayama C, Inoue Y. Transient expression of GABAA receptor alpha2 and alpha3 subunits in differentiating cerebellar neurons. Brain Res Dev Brain Res. 2004;148(2):169-77.
Takayama, C., & Inoue, Y. (2004). Transient expression of GABAA receptor alpha2 and alpha3 subunits in differentiating cerebellar neurons. Brain Research. Developmental Brain Research, 148(2), pp. 169-77.
Takayama C, Inoue Y. Transient Expression of GABAA Receptor Alpha2 and Alpha3 Subunits in Differentiating Cerebellar Neurons. Brain Res Dev Brain Res. 2004 Feb 20;148(2):169-77. PubMed PMID: 14766194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transient expression of GABAA receptor alpha2 and alpha3 subunits in differentiating cerebellar neurons. AU - Takayama,Chitoshi, AU - Inoue,Yoshiro, PY - 2003/11/18/accepted PY - 2004/2/10/pubmed PY - 2004/5/8/medline PY - 2004/2/10/entrez SP - 169 EP - 77 JF - Brain research. Developmental brain research JO - Brain Res. Dev. Brain Res. VL - 148 IS - 2 N2 - In the adult mammalian brain, synaptic transmission mediated by gamma-amino butyric acid (GABA) plays a role in inhibition of excitatory synaptic transmission. During brain development, GABA is involved in brain morphogenesis. To clarify how GABA exerts its effect on immature neurons, we examined the expression of the GABAA receptor alpha2 and alpha3 subunits, which are abundantly expressed before alpha1 and alpha6 subunits appear, in the developing mouse cerebellum using in situ hybridization. Proliferating neuronal precursors in the ventricular zone and external granular layer expressed neither alpha2 nor alpha3 subunits. Hybridization signals for the alpha2 and alpha3 subunit mRNAs first appeared in the differentiating zone at embryonic day 13 (E13). The alpha2 subunit was detected in the migrating and differentiating granule cells and cerebellar nucleus neurons until postnatal day 14 (P14). Hybridization signals for the alpha3 subunit mRNA, on the other hand, were localized in the developing Purkinje cells and cerebellar nucleus neurons, and disappeared from Purkinje cells by the end of first postnatal week. Taken together, this indicated that the alpha2 and alpha3 subunits were abundantly expressed in distinct types of cerebellar neurons after completing cell proliferation while forming the neural network. These results suggest that GABA might extrasynaptically activate the GABAA receptors containing alpha2 and/or alpha3 subunits on the differentiating neurons before finishing the formation of synapses and networks, and could be involved in neuronal differentiation and maturation in the cerebellum. SN - 0165-3806 UR - https://www.unboundmedicine.com/medline/citation/14766194/Transient_expression_of_GABAA_receptor_alpha2_and_alpha3_subunits_in_differentiating_cerebellar_neurons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165380603003493 DB - PRIME DP - Unbound Medicine ER -