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Leukotrienes and 8-isoprostane in exhaled breath condensate of children with stable and unstable asthma.
J Allergy Clin Immunol. 2004 Feb; 113(2):257-63.JA

Abstract

BACKGROUND

Cysteinyl-leukotrienes (cys-LTs) and 8-isoprostane are biomarkers of airway inflammation and oxidative stress.

OBJECTIVE

The aim of this study was to evaluate cys-LT and 8-isoprostane levels in exhaled breath condensate (EBC) of children with different degrees of asthma severity.

METHODS

EBC was collected from 14 steroid-naive children with mild persistent asthma, 13 children with stable mild- to-moderate persistent asthma treated with inhaled corticosteroids (ICS), 9 ICS-treated children with unstable asthma, and 19 healthy children.

RESULTS

In the three groups of asthmatic children, EBC concentrations of cys-LTs and 8-isoprostane were significantly higher than in control children (steroid-naive asthmatic children: cys-LTs median, 10.8 pg/mL, P <.001, 8-isoprostane, 16.2 pg/mL, P <.001; ICS-treated stable asthmatic children: cys-LTs, 12.7 pg/mL, P <.001, 8-isoprostane, 18.1 pg/mL, P <.001; children with unstable asthma: cys-LTs, 106.0 pg/mL, P <.01, 8-isoprostane, 29.7 pg/mL, P <.01; control children: cys-LTs, 4.3 pg/mL, 8-isoprostane, 3.5 pg/mL). Cys-LT levels were higher in children with unstable asthma than in the other two asthmatic groups (P <.05). FE(NO) levels were significantly higher in steroid-naive and in children with unstable asthma compared with ICS-treated children with stable asthma (P <.01).

CONCLUSIONS

Our study shows that EBC cys-LTs and 8-isoprostane concentrations are higher in asthmatic children than in healthy control children, with scattered values in patients with unstable asthma. These findings suggest that EBC eicosanoid measurement may have useful clinical implications for investigating phenotype differences among asthmatic patients.

Authors+Show Affiliations

Department of Pediatrics, University of Padova, Padova, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14767439

Citation

Zanconato, Stefania, et al. "Leukotrienes and 8-isoprostane in Exhaled Breath Condensate of Children With Stable and Unstable Asthma." The Journal of Allergy and Clinical Immunology, vol. 113, no. 2, 2004, pp. 257-63.
Zanconato S, Carraro S, Corradi M, et al. Leukotrienes and 8-isoprostane in exhaled breath condensate of children with stable and unstable asthma. J Allergy Clin Immunol. 2004;113(2):257-63.
Zanconato, S., Carraro, S., Corradi, M., Alinovi, R., Pasquale, M. F., Piacentini, G., Zacchello, F., & Baraldi, E. (2004). Leukotrienes and 8-isoprostane in exhaled breath condensate of children with stable and unstable asthma. The Journal of Allergy and Clinical Immunology, 113(2), 257-63.
Zanconato S, et al. Leukotrienes and 8-isoprostane in Exhaled Breath Condensate of Children With Stable and Unstable Asthma. J Allergy Clin Immunol. 2004;113(2):257-63. PubMed PMID: 14767439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leukotrienes and 8-isoprostane in exhaled breath condensate of children with stable and unstable asthma. AU - Zanconato,Stefania, AU - Carraro,Silvia, AU - Corradi,Massimo, AU - Alinovi,Rossella, AU - Pasquale,Maria Francesca, AU - Piacentini,Giorgio, AU - Zacchello,Franco, AU - Baraldi,Eugenio, PY - 2004/2/10/pubmed PY - 2004/3/18/medline PY - 2004/2/10/entrez SP - 257 EP - 63 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 113 IS - 2 N2 - BACKGROUND: Cysteinyl-leukotrienes (cys-LTs) and 8-isoprostane are biomarkers of airway inflammation and oxidative stress. OBJECTIVE: The aim of this study was to evaluate cys-LT and 8-isoprostane levels in exhaled breath condensate (EBC) of children with different degrees of asthma severity. METHODS: EBC was collected from 14 steroid-naive children with mild persistent asthma, 13 children with stable mild- to-moderate persistent asthma treated with inhaled corticosteroids (ICS), 9 ICS-treated children with unstable asthma, and 19 healthy children. RESULTS: In the three groups of asthmatic children, EBC concentrations of cys-LTs and 8-isoprostane were significantly higher than in control children (steroid-naive asthmatic children: cys-LTs median, 10.8 pg/mL, P <.001, 8-isoprostane, 16.2 pg/mL, P <.001; ICS-treated stable asthmatic children: cys-LTs, 12.7 pg/mL, P <.001, 8-isoprostane, 18.1 pg/mL, P <.001; children with unstable asthma: cys-LTs, 106.0 pg/mL, P <.01, 8-isoprostane, 29.7 pg/mL, P <.01; control children: cys-LTs, 4.3 pg/mL, 8-isoprostane, 3.5 pg/mL). Cys-LT levels were higher in children with unstable asthma than in the other two asthmatic groups (P <.05). FE(NO) levels were significantly higher in steroid-naive and in children with unstable asthma compared with ICS-treated children with stable asthma (P <.01). CONCLUSIONS: Our study shows that EBC cys-LTs and 8-isoprostane concentrations are higher in asthmatic children than in healthy control children, with scattered values in patients with unstable asthma. These findings suggest that EBC eicosanoid measurement may have useful clinical implications for investigating phenotype differences among asthmatic patients. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/14767439/Leukotrienes_and_8_isoprostane_in_exhaled_breath_condensate_of_children_with_stable_and_unstable_asthma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091674903024886 DB - PRIME DP - Unbound Medicine ER -