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Analysis of propolis from the continental and Adriatic regions of Croatia.
Acta Pharm. 2003 Dec; 53(4):275-85.AP

Abstract

Thin-layer chromatography of ethanolic extract of propolis (EEP) from the continental and Adriatic regions of Croatia showed that 72.2% of propolis samples contain galangin, 88.8% of samples contain kaempferol, naringenin and apigenin and 66.6% of samples contain caffeic acid. Caffeic acid, pinocembrin, galangin, chrysin and naringenin were analyzed by HPLC. In all samples, pinocembrin was the dominant flavonoid. In samples from the Adriatic region, concentration of pinocembrin ranged from 0.03 to 6.14% (x = 2.87%) and in the continental region samples from 0 to 4.74% (x = 2.84%). Chrysin was found in all propolis samples in a concentration ranging from 0.22 to 5.32% (x = 1.86%) in the continental region samples and from 0.03 to 3.64% (x = 1.96%) in samples from the Adriatic region. Chrysin was followed by naringenin, ranging from 0 to 1.14% (x = 0.42%) in samples from the Adriatic region and from 0.22 to 2.41% (x = 0.60%) in the continental region samples. Concentration of caffeic acid ranged from 0 to 10.11% (x = 2.69%) in the Adriatic region samples and from 0.27 to 2.67% (x = 1.37%) in samples from the continental region of Croatia. Results of HPLC analyses suggest that propolis samples collected from various parts of Croatia do not differ markedly in contents of chrysin, pinocembrin, naringenin and galangin but differ in the concentration of caffeic acid. All EEPs significantly inhibited the growth of Bacillus subtilis in comparison with the control (80% ethanol) (p < 0.05), showing inhibition zones of 16 +/- 2 mm for samples from the continental region, and of 18 +/- 3 mm for samples from the Adriatic region. There was no significant difference in antimicrobial activity of EEPs from the continental and Adriatic regions of Croatia, suggesting that bactericidal activity depends on synergism of all phenolic compounds.

Authors+Show Affiliations

Institute of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, HR-10000 Zagreb, Croatia. ivan.kosalec@zg.htnet.hrNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

14769234

Citation

Kosalec, Ivan, et al. "Analysis of Propolis From the Continental and Adriatic Regions of Croatia." Acta Pharmaceutica (Zagreb, Croatia), vol. 53, no. 4, 2003, pp. 275-85.
Kosalec I, Bakmaz M, Pepeljnjak S. Analysis of propolis from the continental and Adriatic regions of Croatia. Acta Pharm. 2003;53(4):275-85.
Kosalec, I., Bakmaz, M., & Pepeljnjak, S. (2003). Analysis of propolis from the continental and Adriatic regions of Croatia. Acta Pharmaceutica (Zagreb, Croatia), 53(4), 275-85.
Kosalec I, Bakmaz M, Pepeljnjak S. Analysis of Propolis From the Continental and Adriatic Regions of Croatia. Acta Pharm. 2003;53(4):275-85. PubMed PMID: 14769234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of propolis from the continental and Adriatic regions of Croatia. AU - Kosalec,Ivan, AU - Bakmaz,Marina, AU - Pepeljnjak,Stjepan, PY - 2004/2/11/pubmed PY - 2004/3/9/medline PY - 2004/2/11/entrez SP - 275 EP - 85 JF - Acta pharmaceutica (Zagreb, Croatia) JO - Acta Pharm VL - 53 IS - 4 N2 - Thin-layer chromatography of ethanolic extract of propolis (EEP) from the continental and Adriatic regions of Croatia showed that 72.2% of propolis samples contain galangin, 88.8% of samples contain kaempferol, naringenin and apigenin and 66.6% of samples contain caffeic acid. Caffeic acid, pinocembrin, galangin, chrysin and naringenin were analyzed by HPLC. In all samples, pinocembrin was the dominant flavonoid. In samples from the Adriatic region, concentration of pinocembrin ranged from 0.03 to 6.14% (x = 2.87%) and in the continental region samples from 0 to 4.74% (x = 2.84%). Chrysin was found in all propolis samples in a concentration ranging from 0.22 to 5.32% (x = 1.86%) in the continental region samples and from 0.03 to 3.64% (x = 1.96%) in samples from the Adriatic region. Chrysin was followed by naringenin, ranging from 0 to 1.14% (x = 0.42%) in samples from the Adriatic region and from 0.22 to 2.41% (x = 0.60%) in the continental region samples. Concentration of caffeic acid ranged from 0 to 10.11% (x = 2.69%) in the Adriatic region samples and from 0.27 to 2.67% (x = 1.37%) in samples from the continental region of Croatia. Results of HPLC analyses suggest that propolis samples collected from various parts of Croatia do not differ markedly in contents of chrysin, pinocembrin, naringenin and galangin but differ in the concentration of caffeic acid. All EEPs significantly inhibited the growth of Bacillus subtilis in comparison with the control (80% ethanol) (p < 0.05), showing inhibition zones of 16 +/- 2 mm for samples from the continental region, and of 18 +/- 3 mm for samples from the Adriatic region. There was no significant difference in antimicrobial activity of EEPs from the continental and Adriatic regions of Croatia, suggesting that bactericidal activity depends on synergism of all phenolic compounds. SN - 1330-0075 UR - https://www.unboundmedicine.com/medline/citation/14769234/Analysis_of_propolis_from_the_continental_and_Adriatic_regions_of_Croatia_ DB - PRIME DP - Unbound Medicine ER -