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The role of apolipoprotein E gene polymorphisms in primary open-angle glaucoma.
Arch Ophthalmol. 2004 Feb; 122(2):258-61.AO

Abstract

OBJECTIVE

To test the hypothesis that genetic polymorphisms of the apolipoprotein E (APOE) gene are associated with primary open-angle glaucoma (POAG), based on the association between neurodegenerative diseases and the APOE genotype.

METHODS

Genomic DNA was examined from an unrelated cohort of 137 POAG patients and 75 control subjects from the ophthalmology department of the Royal Victoria Infirmary. The APOE allele frequency (epsilon2, epsilon3, and epsilon4 alleles) was studied by polymerase chain reaction amplification of the related locus (19q13.2), enzymatic digestion of the products, gel electrophoresis, and imaging under UV illumination. For statistical analysis, we used a logistic regression model that included intraocular pressure as a continuous variable to study the possible correlation between POAG and APOE allele frequency.

RESULTS

Logistic regression analysis showed no statistically significant association between the frequency of the APOE allele and POAG for the population studied, irrespective of the IOP (epsilon2 odds ratio, 0.82; 95% confidence interval, 0.12-5.79 [P =.84]; epsilon3 odds ratio, 0.39; 95% confidence interval, 0.10-1.49 [P =.17]; and epsilon4 odds ratio, 3.84; 95% confidence interval, 0.80-18.49 [P =.09]).

CONCLUSION

In our cohort, the APOE genotype does not constitute a risk factor for developing POAG, even in patients with normal-tension glaucoma.Clinical Relevance Apolipoprotein E polymorphisms do not appear to be contributory to POAG.

Authors+Show Affiliations

Ressiniotis T
Department of Ophthalmology, Royal Victoria Infirmary, and Department of Neurology, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, England. tomres@doctors.org.uk
Griffiths PG
No affiliation info available
Birch M
No affiliation info available
Keers S
No affiliation info available
Chinnery PF
No affiliation info available

MeSH

AgedAged, 80 and overAllelesApolipoproteins EGene FrequencyGenotypeGlaucoma, Open-AngleHumansMiddle AgedPolymerase Chain ReactionPolymorphism, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14769603

Citation

Ressiniotis, Thomas, et al. "The Role of Apolipoprotein E Gene Polymorphisms in Primary Open-angle Glaucoma." Archives of Ophthalmology (Chicago, Ill. : 1960), vol. 122, no. 2, 2004, pp. 258-61.
Ressiniotis T, Griffiths PG, Birch M, et al. The role of apolipoprotein E gene polymorphisms in primary open-angle glaucoma. Arch Ophthalmol. 2004;122(2):258-61.
Ressiniotis, T., Griffiths, P. G., Birch, M., Keers, S., & Chinnery, P. F. (2004). The role of apolipoprotein E gene polymorphisms in primary open-angle glaucoma. Archives of Ophthalmology (Chicago, Ill. : 1960), 122(2), 258-61.
Ressiniotis T, et al. The Role of Apolipoprotein E Gene Polymorphisms in Primary Open-angle Glaucoma. Arch Ophthalmol. 2004;122(2):258-61. PubMed PMID: 14769603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of apolipoprotein E gene polymorphisms in primary open-angle glaucoma. AU - Ressiniotis,Thomas, AU - Griffiths,Philip G, AU - Birch,Michael, AU - Keers,Sharon, AU - Chinnery,Patrick F, PY - 2004/2/11/pubmed PY - 2004/3/3/medline PY - 2004/2/11/entrez SP - 258 EP - 61 JF - Archives of ophthalmology (Chicago, Ill. : 1960) JO - Arch Ophthalmol VL - 122 IS - 2 N2 - OBJECTIVE: To test the hypothesis that genetic polymorphisms of the apolipoprotein E (APOE) gene are associated with primary open-angle glaucoma (POAG), based on the association between neurodegenerative diseases and the APOE genotype. METHODS: Genomic DNA was examined from an unrelated cohort of 137 POAG patients and 75 control subjects from the ophthalmology department of the Royal Victoria Infirmary. The APOE allele frequency (epsilon2, epsilon3, and epsilon4 alleles) was studied by polymerase chain reaction amplification of the related locus (19q13.2), enzymatic digestion of the products, gel electrophoresis, and imaging under UV illumination. For statistical analysis, we used a logistic regression model that included intraocular pressure as a continuous variable to study the possible correlation between POAG and APOE allele frequency. RESULTS: Logistic regression analysis showed no statistically significant association between the frequency of the APOE allele and POAG for the population studied, irrespective of the IOP (epsilon2 odds ratio, 0.82; 95% confidence interval, 0.12-5.79 [P =.84]; epsilon3 odds ratio, 0.39; 95% confidence interval, 0.10-1.49 [P =.17]; and epsilon4 odds ratio, 3.84; 95% confidence interval, 0.80-18.49 [P =.09]). CONCLUSION: In our cohort, the APOE genotype does not constitute a risk factor for developing POAG, even in patients with normal-tension glaucoma.Clinical Relevance Apolipoprotein E polymorphisms do not appear to be contributory to POAG. SN - 0003-9950 UR - https://www.unboundmedicine.com/medline/citation/14769603/The_role_of_apolipoprotein_E_gene_polymorphisms_in_primary_open_angle_glaucoma_ DB - PRIME DP - Unbound Medicine ER -