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Contrasting effects of interferon-gamma and interleukin-4 on neopterin generation from human adherent monocytes.
Lymphokine Cytokine Res. 1992 Dec; 11(6):327-30.LC

Abstract

The production of neopterin is closely correlated with activation of cell-mediated immunity. Neopterin appears to be produced by human macrophages specifically stimulated with interferon-gamma (IFN-gamma). Interleukin-4 (IL-4), a B and T stimulatory factor, has recently been shown to inhibit monocyte/macrophage functions, including the ability to suppress monocyte-generated cytokines. In this report we confirmed previous studies that identified the monocyte/macrophage as the main producing cell among human blood cells and that secretion is stimulated by IFN-gamma and lipopolysaccharides (LPS). IL-4 inhibits the generation of neopterin from unstimulated monocytes. This inhibitory effect was dose dependent and occurred at concentrations lower than 0.01 ng/ml. However, IL-4 had only a minimal inhibitory effect on LPS-induced generation of neopterin and could not reverse IFN-gamma-induced neopterin secretion from adherent monocytes. Furthermore, we report that LPS induced IFN-gamma production in monocyte culture. This production is strongly inhibited by IL-4 treatment. These findings indicate that IL-4 can regulate the synthesis of neopterin by adherent blood mononuclear cells and provide further evidence that LPS-induced neopterin in macrophages may act by IFN-gamma-independent mechanisms.

Authors+Show Affiliations

Department of Urology, Osaka University Hospital, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1477185

Citation

Moutabarrik, A, et al. "Contrasting Effects of Interferon-gamma and Interleukin-4 On Neopterin Generation From Human Adherent Monocytes." Lymphokine and Cytokine Research, vol. 11, no. 6, 1992, pp. 327-30.
Moutabarrik A, Takahara S, Namiki M, et al. Contrasting effects of interferon-gamma and interleukin-4 on neopterin generation from human adherent monocytes. Lymphokine Cytokine Res. 1992;11(6):327-30.
Moutabarrik, A., Takahara, S., Namiki, M., Kameoka, H., Seguchi, T., Yokokawa, K., Takano, Y., Sonoda, T., Ishibashi, M., & Zaid, D. (1992). Contrasting effects of interferon-gamma and interleukin-4 on neopterin generation from human adherent monocytes. Lymphokine and Cytokine Research, 11(6), 327-30.
Moutabarrik A, et al. Contrasting Effects of Interferon-gamma and Interleukin-4 On Neopterin Generation From Human Adherent Monocytes. Lymphokine Cytokine Res. 1992;11(6):327-30. PubMed PMID: 1477185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Contrasting effects of interferon-gamma and interleukin-4 on neopterin generation from human adherent monocytes. A1 - Moutabarrik,A, AU - Takahara,S, AU - Namiki,M, AU - Kameoka,H, AU - Seguchi,T, AU - Yokokawa,K, AU - Takano,Y, AU - Sonoda,T, AU - Ishibashi,M, AU - Zaid,D, PY - 1992/12/1/pubmed PY - 1992/12/1/medline PY - 1992/12/1/entrez SP - 327 EP - 30 JF - Lymphokine and cytokine research JO - Lymphokine Cytokine Res. VL - 11 IS - 6 N2 - The production of neopterin is closely correlated with activation of cell-mediated immunity. Neopterin appears to be produced by human macrophages specifically stimulated with interferon-gamma (IFN-gamma). Interleukin-4 (IL-4), a B and T stimulatory factor, has recently been shown to inhibit monocyte/macrophage functions, including the ability to suppress monocyte-generated cytokines. In this report we confirmed previous studies that identified the monocyte/macrophage as the main producing cell among human blood cells and that secretion is stimulated by IFN-gamma and lipopolysaccharides (LPS). IL-4 inhibits the generation of neopterin from unstimulated monocytes. This inhibitory effect was dose dependent and occurred at concentrations lower than 0.01 ng/ml. However, IL-4 had only a minimal inhibitory effect on LPS-induced generation of neopterin and could not reverse IFN-gamma-induced neopterin secretion from adherent monocytes. Furthermore, we report that LPS induced IFN-gamma production in monocyte culture. This production is strongly inhibited by IL-4 treatment. These findings indicate that IL-4 can regulate the synthesis of neopterin by adherent blood mononuclear cells and provide further evidence that LPS-induced neopterin in macrophages may act by IFN-gamma-independent mechanisms. SN - 1056-5477 UR - https://www.unboundmedicine.com/medline/citation/1477185/Contrasting_effects_of_interferon_gamma_and_interleukin_4_on_neopterin_generation_from_human_adherent_monocytes_ DB - PRIME DP - Unbound Medicine ER -