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Contrasting effects of interferon-gamma and interleukin-4 on neopterin generation from human adherent monocytes.
Lymphokine Cytokine Res. 1992 Dec; 11(6):327-30.LC

Abstract

The production of neopterin is closely correlated with activation of cell-mediated immunity. Neopterin appears to be produced by human macrophages specifically stimulated with interferon-gamma (IFN-gamma). Interleukin-4 (IL-4), a B and T stimulatory factor, has recently been shown to inhibit monocyte/macrophage functions, including the ability to suppress monocyte-generated cytokines. In this report we confirmed previous studies that identified the monocyte/macrophage as the main producing cell among human blood cells and that secretion is stimulated by IFN-gamma and lipopolysaccharides (LPS). IL-4 inhibits the generation of neopterin from unstimulated monocytes. This inhibitory effect was dose dependent and occurred at concentrations lower than 0.01 ng/ml. However, IL-4 had only a minimal inhibitory effect on LPS-induced generation of neopterin and could not reverse IFN-gamma-induced neopterin secretion from adherent monocytes. Furthermore, we report that LPS induced IFN-gamma production in monocyte culture. This production is strongly inhibited by IL-4 treatment. These findings indicate that IL-4 can regulate the synthesis of neopterin by adherent blood mononuclear cells and provide further evidence that LPS-induced neopterin in macrophages may act by IFN-gamma-independent mechanisms.

Authors+Show Affiliations

Moutabarrik A
Department of Urology, Osaka University Hospital, Japan.
Takahara S
No affiliation info available
Namiki M
No affiliation info available
Kameoka H
No affiliation info available
Seguchi T
No affiliation info available
Yokokawa K
No affiliation info available
Takano Y
No affiliation info available
Sonoda T
No affiliation info available
Ishibashi M
No affiliation info available
Zaid D
No affiliation info available

MeSH

BiopterinCell AdhesionHumansIn Vitro TechniquesInterferon-gammaInterleukin-4LipopolysaccharidesMonocytesNeopterin

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1477185

Citation

Moutabarrik, A, et al. "Contrasting Effects of Interferon-gamma and Interleukin-4 On Neopterin Generation From Human Adherent Monocytes." Lymphokine and Cytokine Research, vol. 11, no. 6, 1992, pp. 327-30.
Moutabarrik A, Takahara S, Namiki M, et al. Contrasting effects of interferon-gamma and interleukin-4 on neopterin generation from human adherent monocytes. Lymphokine Cytokine Res. 1992;11(6):327-30.
Moutabarrik, A., Takahara, S., Namiki, M., Kameoka, H., Seguchi, T., Yokokawa, K., Takano, Y., Sonoda, T., Ishibashi, M., & Zaid, D. (1992). Contrasting effects of interferon-gamma and interleukin-4 on neopterin generation from human adherent monocytes. Lymphokine and Cytokine Research, 11(6), 327-30.
Moutabarrik A, et al. Contrasting Effects of Interferon-gamma and Interleukin-4 On Neopterin Generation From Human Adherent Monocytes. Lymphokine Cytokine Res. 1992;11(6):327-30. PubMed PMID: 1477185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Contrasting effects of interferon-gamma and interleukin-4 on neopterin generation from human adherent monocytes. A1 - Moutabarrik,A, AU - Takahara,S, AU - Namiki,M, AU - Kameoka,H, AU - Seguchi,T, AU - Yokokawa,K, AU - Takano,Y, AU - Sonoda,T, AU - Ishibashi,M, AU - Zaid,D, PY - 1992/12/1/pubmed PY - 1992/12/1/medline PY - 1992/12/1/entrez SP - 327 EP - 30 JF - Lymphokine and cytokine research JO - Lymphokine Cytokine Res. VL - 11 IS - 6 N2 - The production of neopterin is closely correlated with activation of cell-mediated immunity. Neopterin appears to be produced by human macrophages specifically stimulated with interferon-gamma (IFN-gamma). Interleukin-4 (IL-4), a B and T stimulatory factor, has recently been shown to inhibit monocyte/macrophage functions, including the ability to suppress monocyte-generated cytokines. In this report we confirmed previous studies that identified the monocyte/macrophage as the main producing cell among human blood cells and that secretion is stimulated by IFN-gamma and lipopolysaccharides (LPS). IL-4 inhibits the generation of neopterin from unstimulated monocytes. This inhibitory effect was dose dependent and occurred at concentrations lower than 0.01 ng/ml. However, IL-4 had only a minimal inhibitory effect on LPS-induced generation of neopterin and could not reverse IFN-gamma-induced neopterin secretion from adherent monocytes. Furthermore, we report that LPS induced IFN-gamma production in monocyte culture. This production is strongly inhibited by IL-4 treatment. These findings indicate that IL-4 can regulate the synthesis of neopterin by adherent blood mononuclear cells and provide further evidence that LPS-induced neopterin in macrophages may act by IFN-gamma-independent mechanisms. SN - 1056-5477 UR - https://www.unboundmedicine.com/medline/citation/1477185/Contrasting_effects_of_interferon_gamma_and_interleukin_4_on_neopterin_generation_from_human_adherent_monocytes_ DB - PRIME DP - Unbound Medicine ER -