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Cloning and transgenesis in mammals: implications for xenotransplantation.
Am J Transplant. 2004; 4 Suppl 6:43-50.AJ

Abstract

Availability of suitable organs for transplantation remains of major concern and projections indicate that the problem will continue to increase. Therefore, alternatives to the use of human organs for transplantation, continue to be explored including use of stem cells, artificial organs, and organs from other species (xenotransplantation). In xenotransplantation, the species of choice remains the pig due to its physiological similarities to humans, reduced costs, ease of manipulation, and reduced ethical concerns to its use. However, in order to develop pig organs that are suitable for xenotransplantation, complex genetic modification need to be undertaken. These modifications require the introduction of precise genetic changes into the pig that can only be accomplished at this time using somatic cell nuclear transfer. We cover in this review advances in transgenic manipulation and cloning in swine and how the development of these two technologies is critical to the eventual utilization of the pig as a human organ donor.

Authors+Show Affiliations

Piedrahita JA
Department of Molecular Biomedical Sciences, College Of Veterinary Medicine, North Carolina State University, USA. jorge_piedrahita@ncsu.edu
Mir B
No affiliation info available

MeSH

AnimalsAnimals, Genetically ModifiedCloning, OrganismEmbryo, MammalianGraft RejectionHumansImmune ToleranceImmunosuppression TherapyNuclear Transfer TechniquesStem CellsSwineTransplantation, Heterologous

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

14871273

Citation

Piedrahita, Jorge A., and Bashir Mir. "Cloning and Transgenesis in Mammals: Implications for Xenotransplantation." American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, vol. 4 Suppl 6, 2004, pp. 43-50.
Piedrahita JA, Mir B. Cloning and transgenesis in mammals: implications for xenotransplantation. Am J Transplant. 2004;4 Suppl 6:43-50.
Piedrahita, J. A., & Mir, B. (2004). Cloning and transgenesis in mammals: implications for xenotransplantation. American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 4 Suppl 6, 43-50.
Piedrahita JA, Mir B. Cloning and Transgenesis in Mammals: Implications for Xenotransplantation. Am J Transplant. 2004;4 Suppl 6:43-50. PubMed PMID: 14871273.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and transgenesis in mammals: implications for xenotransplantation. AU - Piedrahita,Jorge A, AU - Mir,Bashir, PY - 2004/2/12/pubmed PY - 2004/10/1/medline PY - 2004/2/12/entrez SP - 43 EP - 50 JF - American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons JO - Am J Transplant VL - 4 Suppl 6 N2 - Availability of suitable organs for transplantation remains of major concern and projections indicate that the problem will continue to increase. Therefore, alternatives to the use of human organs for transplantation, continue to be explored including use of stem cells, artificial organs, and organs from other species (xenotransplantation). In xenotransplantation, the species of choice remains the pig due to its physiological similarities to humans, reduced costs, ease of manipulation, and reduced ethical concerns to its use. However, in order to develop pig organs that are suitable for xenotransplantation, complex genetic modification need to be undertaken. These modifications require the introduction of precise genetic changes into the pig that can only be accomplished at this time using somatic cell nuclear transfer. We cover in this review advances in transgenic manipulation and cloning in swine and how the development of these two technologies is critical to the eventual utilization of the pig as a human organ donor. SN - 1600-6135 UR - https://www.unboundmedicine.com/medline/citation/14871273/Cloning_and_transgenesis_in_mammals:_implications_for_xenotransplantation_ DB - PRIME DP - Unbound Medicine ER -