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Proliferative and cytotoxic response of human natural killer cells exposed to transporter associated with antigen-processing-deficient cells.
Scand J Immunol. 2004 Feb; 59(2):159-67.SJ

Abstract

In transporter associated with antigen-processing (TAP)-deficient patients affected by a severe downmodulation of human leucocyte antigen class I (HLA-I) molecules, natural killer (NK) cells have an increased expression of the inhibitory receptor CD94/NKG2A. Focusing our attention on NK cells, we have investigated the phenotype, function and proliferative response of peripheral blood lymphocytes (PBLs) derived from healthy donors after coculturing with TAP (T2)- or HLA-I-deficient (721.221) cell lines and their related HLA-I-expressing transfectants (T3 and DT360, respectively). After 4 days, NK cells cocultured with T2 cells had a threefold increased CD94 expression compared to NK cells cocultured with T3. This increase was due to proliferation of the CD56brightCD94bright subset. In contrast, expression of other inhibitory receptors [killer cell immunoglobulin (Ig)-like receptors] was variable during time and was not related to HLA-I molecules expressed by stimulating cells. Similar results were obtained using HLA-I-deficient cells (721.221). The PBLs cocultured for 4 days with T2 cells displayed enhanced cytotoxic responses. The results suggest that CD56brightCD94bright NK cells are induced to proliferate and kill in response to a TAP-deficient environment. The changes seen in the NK-cell compartment were partially contributed by T lymphocytes present in the coculture. These data could explain the increased CD94 expression and autoimmune manifestations observed in TAP-deficient patients.

Authors+Show Affiliations

Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14871292

Citation

Cerboni, C, et al. "Proliferative and Cytotoxic Response of Human Natural Killer Cells Exposed to Transporter Associated With Antigen-processing-deficient Cells." Scandinavian Journal of Immunology, vol. 59, no. 2, 2004, pp. 159-67.
Cerboni C, Oberg L, Terrazzano G, et al. Proliferative and cytotoxic response of human natural killer cells exposed to transporter associated with antigen-processing-deficient cells. Scand J Immunol. 2004;59(2):159-67.
Cerboni, C., Oberg, L., Terrazzano, G., Zappacosta, S., Carbone, E., & Kärre, K. (2004). Proliferative and cytotoxic response of human natural killer cells exposed to transporter associated with antigen-processing-deficient cells. Scandinavian Journal of Immunology, 59(2), 159-67.
Cerboni C, et al. Proliferative and Cytotoxic Response of Human Natural Killer Cells Exposed to Transporter Associated With Antigen-processing-deficient Cells. Scand J Immunol. 2004;59(2):159-67. PubMed PMID: 14871292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proliferative and cytotoxic response of human natural killer cells exposed to transporter associated with antigen-processing-deficient cells. AU - Cerboni,C, AU - Oberg,L, AU - Terrazzano,G, AU - Zappacosta,S, AU - Carbone,E, AU - Kärre,K, PY - 2004/2/12/pubmed PY - 2004/3/12/medline PY - 2004/2/12/entrez SP - 159 EP - 67 JF - Scandinavian journal of immunology JO - Scand J Immunol VL - 59 IS - 2 N2 - In transporter associated with antigen-processing (TAP)-deficient patients affected by a severe downmodulation of human leucocyte antigen class I (HLA-I) molecules, natural killer (NK) cells have an increased expression of the inhibitory receptor CD94/NKG2A. Focusing our attention on NK cells, we have investigated the phenotype, function and proliferative response of peripheral blood lymphocytes (PBLs) derived from healthy donors after coculturing with TAP (T2)- or HLA-I-deficient (721.221) cell lines and their related HLA-I-expressing transfectants (T3 and DT360, respectively). After 4 days, NK cells cocultured with T2 cells had a threefold increased CD94 expression compared to NK cells cocultured with T3. This increase was due to proliferation of the CD56brightCD94bright subset. In contrast, expression of other inhibitory receptors [killer cell immunoglobulin (Ig)-like receptors] was variable during time and was not related to HLA-I molecules expressed by stimulating cells. Similar results were obtained using HLA-I-deficient cells (721.221). The PBLs cocultured for 4 days with T2 cells displayed enhanced cytotoxic responses. The results suggest that CD56brightCD94bright NK cells are induced to proliferate and kill in response to a TAP-deficient environment. The changes seen in the NK-cell compartment were partially contributed by T lymphocytes present in the coculture. These data could explain the increased CD94 expression and autoimmune manifestations observed in TAP-deficient patients. SN - 0300-9475 UR - https://www.unboundmedicine.com/medline/citation/14871292/Proliferative_and_cytotoxic_response_of_human_natural_killer_cells_exposed_to_transporter_associated_with_antigen_processing_deficient_cells_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0300-9475&date=2004&volume=59&issue=2&spage=159 DB - PRIME DP - Unbound Medicine ER -