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Characterization of two genes expressed in the salivary glands of the Hessian fly, Mayetiola destructor (Say).
Insect Biochem Mol Biol. 2004 Mar; 34(3):229-37.IB

Abstract

Two genes, SSGP-11A1 and SSGP-12A1, have been isolated that encodes proteins with a secretion signal peptide at theN-terminals from the Hessian fly (Mayetiola destructor (Say)). The SSGP-11A1 gene contains one small intron (89 bp) and encodes a putative protein with 79 amino acids. The first 18 amino acids constitute a putative secretion signal peptide. The SSGP-12A1 gene contains three small introns and encodes a putative protein with 234 amino acids. The first 19 amino acids constitute a putative secretion signal peptide. Northern blot analysis revealed that both of the genes are primarily expressed in the salivary glands of Hessian fly larvae, the feeding stage of the insect. These observations are consistent with the possibility that the proteins encoded by them are secreted into host plants during feeding. Even though both genes are exclusively expressed in Hessian fly larvae, the expression profiles between them were quite different in insects at different instars. The SSGP-11A1 gene was expressed in all instars of larvae while the SSGP-12A1 gene was almost exclusively expressed in the first instar larvae. The differential expression suggests that the proteins encoded by these two genes may perform different functions. In situ hybridization revealed that the SSGP-11A1 gene is located on the short arm of chromosome A1 while SSGP-12A1 gene is on the long arm of chromosome A2.

Authors+Show Affiliations

Department of Entomology, Kansas State University, 123 Waters Hall, Manhattan, KS 66506, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

14871619

Citation

Liu, Xuming, et al. "Characterization of Two Genes Expressed in the Salivary Glands of the Hessian Fly, Mayetiola Destructor (Say)." Insect Biochemistry and Molecular Biology, vol. 34, no. 3, 2004, pp. 229-37.
Liu X, Fellers JP, Wilde GE, et al. Characterization of two genes expressed in the salivary glands of the Hessian fly, Mayetiola destructor (Say). Insect Biochem Mol Biol. 2004;34(3):229-37.
Liu, X., Fellers, J. P., Wilde, G. E., Stuart, J. J., & Chen, M. S. (2004). Characterization of two genes expressed in the salivary glands of the Hessian fly, Mayetiola destructor (Say). Insect Biochemistry and Molecular Biology, 34(3), 229-37.
Liu X, et al. Characterization of Two Genes Expressed in the Salivary Glands of the Hessian Fly, Mayetiola Destructor (Say). Insect Biochem Mol Biol. 2004;34(3):229-37. PubMed PMID: 14871619.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of two genes expressed in the salivary glands of the Hessian fly, Mayetiola destructor (Say). AU - Liu,Xuming, AU - Fellers,John P, AU - Wilde,Gerald E, AU - Stuart,Jeffrey J, AU - Chen,Ming-Shun, PY - 2003/08/11/received PY - 2003/10/28/revised PY - 2003/10/28/accepted PY - 2004/2/12/pubmed PY - 2004/5/28/medline PY - 2004/2/12/entrez SP - 229 EP - 37 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 34 IS - 3 N2 - Two genes, SSGP-11A1 and SSGP-12A1, have been isolated that encodes proteins with a secretion signal peptide at theN-terminals from the Hessian fly (Mayetiola destructor (Say)). The SSGP-11A1 gene contains one small intron (89 bp) and encodes a putative protein with 79 amino acids. The first 18 amino acids constitute a putative secretion signal peptide. The SSGP-12A1 gene contains three small introns and encodes a putative protein with 234 amino acids. The first 19 amino acids constitute a putative secretion signal peptide. Northern blot analysis revealed that both of the genes are primarily expressed in the salivary glands of Hessian fly larvae, the feeding stage of the insect. These observations are consistent with the possibility that the proteins encoded by them are secreted into host plants during feeding. Even though both genes are exclusively expressed in Hessian fly larvae, the expression profiles between them were quite different in insects at different instars. The SSGP-11A1 gene was expressed in all instars of larvae while the SSGP-12A1 gene was almost exclusively expressed in the first instar larvae. The differential expression suggests that the proteins encoded by these two genes may perform different functions. In situ hybridization revealed that the SSGP-11A1 gene is located on the short arm of chromosome A1 while SSGP-12A1 gene is on the long arm of chromosome A2. SN - 0965-1748 UR - https://www.unboundmedicine.com/medline/citation/14871619/Characterization_of_two_genes_expressed_in_the_salivary_glands_of_the_Hessian_fly_Mayetiola_destructor__Say__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965174803001942 DB - PRIME DP - Unbound Medicine ER -