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Practical uses of acridine orange fluorescence microscopy of centrifuged blood (QBC Malaria Test) and the QBCII Hematology System in patients attending malaria clinics in Thailand.
Southeast Asian J Trop Med Public Health. 1992 Sep; 23(3):406-13.SA

Abstract

We evaluated the detection of malaria parasites using acridine orange fluorescence microscopy of centrifuged blood (AOFM/CB or "QBC Malaria Test") at two government malaria clinics in rural Thailand. In a subgroup of the patients, a QBC Hematology System for the determination of complete blood counts was also utilized. A Giemsa-stained thick smear (GTS) reading of 100 (1,000x) microscopic fields was used as standard. The AOFM/CB sensitivities were 97% overall and 95% for P. falciparum (Pf). Sensitivity was lower for P. vivax (Pv) (76%). Pv sensitivity depended largely on ameboid form density. A threshold for AOFM/CB to consistently detect Pv ameboid forms was estimated to be 10/100 WBC (700/microliters blood). AOFM/CB was capable of detecting Pf gametocytes and schizonts more frequently than GTS. The total Pf rings per microliter blood estimated from GTS was highly correlated with the number of Pf rings per Paralens microscopic field (PMF) suggesting that AOFM/CB could be used quantitatively. From a technical standpoint, the rural tropical settings of Thailand in this study were not an obstacle to the use of QBC Hematology. The system was found to be useful in conjunction with AOFM/CB. However, in patients heavily infected with Pf gametocytes of Pv ameboid forms, their total WBC and lymphocyte counts needed to be appropriately corrected. Overall, AOFM/CB appears to be a promising tool for field diagnosis of malaria if it is affordable to developing countries.

Authors+Show Affiliations

Wongsrichanalai C
Department of Immunology and Biochemistry, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Chuanak N
No affiliation info available
Webster HK
No affiliation info available
Prasittisuk M
No affiliation info available

MeSH

Acridine OrangeEvaluation Studies as TopicHumansMalaria, FalciparumMalaria, VivaxMicroscopy, FluorescenceMyanmarSensitivity and SpecificityThailand

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

1488693

Citation

Wongsrichanalai, C, et al. "Practical Uses of Acridine Orange Fluorescence Microscopy of Centrifuged Blood (QBC Malaria Test) and the QBCII Hematology System in Patients Attending Malaria Clinics in Thailand." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 23, no. 3, 1992, pp. 406-13.
Wongsrichanalai C, Chuanak N, Webster HK, et al. Practical uses of acridine orange fluorescence microscopy of centrifuged blood (QBC Malaria Test) and the QBCII Hematology System in patients attending malaria clinics in Thailand. Southeast Asian J Trop Med Public Health. 1992;23(3):406-13.
Wongsrichanalai, C., Chuanak, N., Webster, H. K., & Prasittisuk, M. (1992). Practical uses of acridine orange fluorescence microscopy of centrifuged blood (QBC Malaria Test) and the QBCII Hematology System in patients attending malaria clinics in Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health, 23(3), 406-13.
Wongsrichanalai C, et al. Practical Uses of Acridine Orange Fluorescence Microscopy of Centrifuged Blood (QBC Malaria Test) and the QBCII Hematology System in Patients Attending Malaria Clinics in Thailand. Southeast Asian J Trop Med Public Health. 1992;23(3):406-13. PubMed PMID: 1488693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Practical uses of acridine orange fluorescence microscopy of centrifuged blood (QBC Malaria Test) and the QBCII Hematology System in patients attending malaria clinics in Thailand. AU - Wongsrichanalai,C, AU - Chuanak,N, AU - Webster,H K, AU - Prasittisuk,M, PY - 1992/9/1/pubmed PY - 1992/9/1/medline PY - 1992/9/1/entrez SP - 406 EP - 13 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 23 IS - 3 N2 - We evaluated the detection of malaria parasites using acridine orange fluorescence microscopy of centrifuged blood (AOFM/CB or "QBC Malaria Test") at two government malaria clinics in rural Thailand. In a subgroup of the patients, a QBC Hematology System for the determination of complete blood counts was also utilized. A Giemsa-stained thick smear (GTS) reading of 100 (1,000x) microscopic fields was used as standard. The AOFM/CB sensitivities were 97% overall and 95% for P. falciparum (Pf). Sensitivity was lower for P. vivax (Pv) (76%). Pv sensitivity depended largely on ameboid form density. A threshold for AOFM/CB to consistently detect Pv ameboid forms was estimated to be 10/100 WBC (700/microliters blood). AOFM/CB was capable of detecting Pf gametocytes and schizonts more frequently than GTS. The total Pf rings per microliter blood estimated from GTS was highly correlated with the number of Pf rings per Paralens microscopic field (PMF) suggesting that AOFM/CB could be used quantitatively. From a technical standpoint, the rural tropical settings of Thailand in this study were not an obstacle to the use of QBC Hematology. The system was found to be useful in conjunction with AOFM/CB. However, in patients heavily infected with Pf gametocytes of Pv ameboid forms, their total WBC and lymphocyte counts needed to be appropriately corrected. Overall, AOFM/CB appears to be a promising tool for field diagnosis of malaria if it is affordable to developing countries. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/1488693/Practical_uses_of_acridine_orange_fluorescence_microscopy_of_centrifuged_blood__QBC_Malaria_Test__and_the_QBCII_Hematology_System_in_patients_attending_malaria_clinics_in_Thailand_ DB - PRIME DP - Unbound Medicine ER -