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The putative sigma factor KatF (RpoS) is required for the transcription of the Salmonella typhimurium virulence gene spvB in Escherichia coli.
FEMS Microbiol Lett. 1992 Dec 01; 78(2-3):271-6.FM

Abstract

The virulence of Salmonella typhimurium for mice is dependent on a plasmid-borne gene cluster termed spv. We previously determined that both S. typhimurium and Escherichia coli bacteria grown in a rich medium preferentially express the spv genes during the stationary phase of growth. In this study we evaluated the role of KatF, a putative sigma factor for starvation- and stationary phase-induced genes, in the expression of the spvB gene. The transcription of spvB in E. coli was compared in katF and wild-type backgrounds, using cloned spvB-lacZ and spvB-cat fusions. Expression of spvB was found to be greatly affected in katF mutants. Complementation experiments performed with the cloned katF gene confirmed that KatF is required for the expression of the S. typhimurium virulence gene spvB in E. coli.

Authors+Show Affiliations

Institut Pasteur, Unité des Entérobactéries, Unité INSERM 199, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1490610

Citation

Norel, F, et al. "The Putative Sigma Factor KatF (RpoS) Is Required for the Transcription of the Salmonella Typhimurium Virulence Gene spvB in Escherichia Coli." FEMS Microbiology Letters, vol. 78, no. 2-3, 1992, pp. 271-6.
Norel F, Robbe-Saule V, Popoff MY, et al. The putative sigma factor KatF (RpoS) is required for the transcription of the Salmonella typhimurium virulence gene spvB in Escherichia coli. FEMS Microbiol Lett. 1992;78(2-3):271-6.
Norel, F., Robbe-Saule, V., Popoff, M. Y., & Coynault, C. (1992). The putative sigma factor KatF (RpoS) is required for the transcription of the Salmonella typhimurium virulence gene spvB in Escherichia coli. FEMS Microbiology Letters, 78(2-3), 271-6.
Norel F, et al. The Putative Sigma Factor KatF (RpoS) Is Required for the Transcription of the Salmonella Typhimurium Virulence Gene spvB in Escherichia Coli. FEMS Microbiol Lett. 1992 Dec 1;78(2-3):271-6. PubMed PMID: 1490610.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The putative sigma factor KatF (RpoS) is required for the transcription of the Salmonella typhimurium virulence gene spvB in Escherichia coli. AU - Norel,F, AU - Robbe-Saule,V, AU - Popoff,M Y, AU - Coynault,C, PY - 1992/12/1/pubmed PY - 1992/12/1/medline PY - 1992/12/1/entrez SP - 271 EP - 6 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 78 IS - 2-3 N2 - The virulence of Salmonella typhimurium for mice is dependent on a plasmid-borne gene cluster termed spv. We previously determined that both S. typhimurium and Escherichia coli bacteria grown in a rich medium preferentially express the spv genes during the stationary phase of growth. In this study we evaluated the role of KatF, a putative sigma factor for starvation- and stationary phase-induced genes, in the expression of the spvB gene. The transcription of spvB in E. coli was compared in katF and wild-type backgrounds, using cloned spvB-lacZ and spvB-cat fusions. Expression of spvB was found to be greatly affected in katF mutants. Complementation experiments performed with the cloned katF gene confirmed that KatF is required for the expression of the S. typhimurium virulence gene spvB in E. coli. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/1490610/The_putative_sigma_factor_KatF__RpoS__is_required_for_the_transcription_of_the_Salmonella_typhimurium_virulence_gene_spvB_in_Escherichia_coli_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1016/0378-1097(92)90039-q DB - PRIME DP - Unbound Medicine ER -