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Fatty acid composition and properties of the liver microsomal membrane of rats fed diets enriched with cholesterol.
J Biochem. 1992 Oct; 112(4):562-7.JB

Abstract

Male rats were fed diets containing olive (OO) or evening primrose (EPO) oil (10% w/w), with or without added cholesterol (1% w/w). After 6-week feeding, the lipid and fatty acid compositions, fluidity, and fatty acid desaturating and cholesterol biosynthesis/esterification related enzymes of liver microsomes were determined. Both the OO and EPO diets, without added cholesterol, increased the contents of oleic and arachidonic acids, respectively, of rat liver microsomes. The results were consistent with the increases in delta 9 and delta 6 desaturation of n-6 essential fatty acids and the lower microviscosity in the EPO group. Dietary cholesterol led to an increase in the cholesterol content of liver microsomes as well as that of phosphatidylcholine (PC). The cholesterol/phospholipid and PC/PE (phosphatidylethanolamine) ratios were also elevated. Fatty acid composition changes were expressed as the accumulation of monounsaturated fatty acids, with accompanying milder depletion of saturated fatty acids in rat liver microsomes. In addition, the arachidonic acid content was lowered, with a concomitant increase in linoleic acid, which led to a significant decrease in the 20:4/18:2 ratio in comparison to in animals fed the cholesterol-free diets. Cholesterol feeding also increased delta 9 desaturase activity as well as membrane microviscosity, whereas it decreased delta 6 and delta 5 desaturase activities. There was a very strong correlation between fluidity and the unsaturation index reduction in the membrane. Furthermore, the activity of hydroxymethylglutaryl-CoA reductase increased and the activity of acyl-CoA:cholesterol acyltransferase decreased in liver microsomes from both cholesterol-fed groups.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Instituto de la Grasa y sus Derivados (CSIC), Sevilla, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1491012

Citation

Muriana, F J., et al. "Fatty Acid Composition and Properties of the Liver Microsomal Membrane of Rats Fed Diets Enriched With Cholesterol." Journal of Biochemistry, vol. 112, no. 4, 1992, pp. 562-7.
Muriana FJ, Vazquez CM, Ruiz-Gutierrez V. Fatty acid composition and properties of the liver microsomal membrane of rats fed diets enriched with cholesterol. J Biochem. 1992;112(4):562-7.
Muriana, F. J., Vazquez, C. M., & Ruiz-Gutierrez, V. (1992). Fatty acid composition and properties of the liver microsomal membrane of rats fed diets enriched with cholesterol. Journal of Biochemistry, 112(4), 562-7.
Muriana FJ, Vazquez CM, Ruiz-Gutierrez V. Fatty Acid Composition and Properties of the Liver Microsomal Membrane of Rats Fed Diets Enriched With Cholesterol. J Biochem. 1992;112(4):562-7. PubMed PMID: 1491012.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fatty acid composition and properties of the liver microsomal membrane of rats fed diets enriched with cholesterol. AU - Muriana,F J, AU - Vazquez,C M, AU - Ruiz-Gutierrez,V, PY - 1992/10/1/pubmed PY - 1992/10/1/medline PY - 1992/10/1/entrez SP - 562 EP - 7 JF - Journal of biochemistry JO - J Biochem VL - 112 IS - 4 N2 - Male rats were fed diets containing olive (OO) or evening primrose (EPO) oil (10% w/w), with or without added cholesterol (1% w/w). After 6-week feeding, the lipid and fatty acid compositions, fluidity, and fatty acid desaturating and cholesterol biosynthesis/esterification related enzymes of liver microsomes were determined. Both the OO and EPO diets, without added cholesterol, increased the contents of oleic and arachidonic acids, respectively, of rat liver microsomes. The results were consistent with the increases in delta 9 and delta 6 desaturation of n-6 essential fatty acids and the lower microviscosity in the EPO group. Dietary cholesterol led to an increase in the cholesterol content of liver microsomes as well as that of phosphatidylcholine (PC). The cholesterol/phospholipid and PC/PE (phosphatidylethanolamine) ratios were also elevated. Fatty acid composition changes were expressed as the accumulation of monounsaturated fatty acids, with accompanying milder depletion of saturated fatty acids in rat liver microsomes. In addition, the arachidonic acid content was lowered, with a concomitant increase in linoleic acid, which led to a significant decrease in the 20:4/18:2 ratio in comparison to in animals fed the cholesterol-free diets. Cholesterol feeding also increased delta 9 desaturase activity as well as membrane microviscosity, whereas it decreased delta 6 and delta 5 desaturase activities. There was a very strong correlation between fluidity and the unsaturation index reduction in the membrane. Furthermore, the activity of hydroxymethylglutaryl-CoA reductase increased and the activity of acyl-CoA:cholesterol acyltransferase decreased in liver microsomes from both cholesterol-fed groups.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0021-924X UR - https://www.unboundmedicine.com/medline/citation/1491012/Fatty_acid_composition_and_properties_of_the_liver_microsomal_membrane_of_rats_fed_diets_enriched_with_cholesterol_ L2 - https://joi.jlc.jst.go.jp/JST.Journalarchive/biochemistry1922/112.562?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -