Tags

Type your tag names separated by a space and hit enter

On-site diagnosis of Plasmodium falciparum, P. vivax, and P. malariae by using the Quantitative Buffy Coat system.
J Parasitol. 1992 Dec; 78(6):994-8.JP

Abstract

The Quantitative Buffy Coat (QBC) system was used for the detection and identification of malaria parasites in blood specimens from 570 residents of Oksibil, an isolated highland valley in the eastern Jayawijaya Mountains of Irian Jaya (Indonesian New Guinea). The availability of a battery-powered centrifuge and a fiberoptic Paralens enabled us to complete and interpret the assay in this remote environment. Of 322 QBC tubes examined for 2-4 min each, results of 295 (92%) concurred with findings on the matched Giemsa-stained thick smear (GTS). The 27 discrepant results included 13 QBC+/GTS- that, upon reexamination, were found to be GTS+. When using the corrected GTS results as the standard, the sensitivity and specificity of the QBC were 94% and 96%, respectively. Because electricity was available only 3 hr per day, it was decided to decrease the examination for an additional 248 QBC to a maximum of 90 sec per tube. This shortened inspection time resulted in a reduction of sensitivity to 53% but specificity was preserved at 89%. Forty-two of 45 conflicting results, QBC-/GTS+ from cases of light Plasmodium falciparum infections with < 1 trophozoite or gametocyte per field, were resolved by reexamination of the QBC in the laboratory. Tubes held at 4 C could be reexamined, without noticeable loss of fluorescence, for at least 6 wk after collection. Despite some difficulty in the identification of Plasmodium species, it was concluded that the QBC is an easy, sensitive method for the rapid diagnosis of malaria in the field and that it provides the inexperienced microscopist with an additional means for on-site identification of individuals needing treatment.

Authors+Show Affiliations

Anthony RL
United States Naval Medical Research Unit No. 2, Jakarta, Indonesia.
Bangs MJ
No affiliation info available
Anthony JM
No affiliation info available
Purnomo
No affiliation info available

MeSH

AdolescentAdultAnimalsChildChild, PreschoolHumansInfantMalariaMalaria, FalciparumMalaria, VivaxMaleMicroscopy, FluorescencePlasmodium falciparumPlasmodium malariaePlasmodium vivaxSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

1491313

Citation

Anthony, R L., et al. "On-site Diagnosis of Plasmodium Falciparum, P. Vivax, and P. Malariae By Using the Quantitative Buffy Coat System." The Journal of Parasitology, vol. 78, no. 6, 1992, pp. 994-8.
Anthony RL, Bangs MJ, Anthony JM, et al. On-site diagnosis of Plasmodium falciparum, P. vivax, and P. malariae by using the Quantitative Buffy Coat system. J Parasitol. 1992;78(6):994-8.
Anthony, R. L., Bangs, M. J., Anthony, J. M., & Purnomo, . (1992). On-site diagnosis of Plasmodium falciparum, P. vivax, and P. malariae by using the Quantitative Buffy Coat system. The Journal of Parasitology, 78(6), 994-8.
Anthony RL, et al. On-site Diagnosis of Plasmodium Falciparum, P. Vivax, and P. Malariae By Using the Quantitative Buffy Coat System. J Parasitol. 1992;78(6):994-8. PubMed PMID: 1491313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-site diagnosis of Plasmodium falciparum, P. vivax, and P. malariae by using the Quantitative Buffy Coat system. AU - Anthony,R L, AU - Bangs,M J, AU - Anthony,J M, AU - Purnomo,, PY - 1992/12/1/pubmed PY - 1992/12/1/medline PY - 1992/12/1/entrez SP - 994 EP - 8 JF - The Journal of parasitology JO - J Parasitol VL - 78 IS - 6 N2 - The Quantitative Buffy Coat (QBC) system was used for the detection and identification of malaria parasites in blood specimens from 570 residents of Oksibil, an isolated highland valley in the eastern Jayawijaya Mountains of Irian Jaya (Indonesian New Guinea). The availability of a battery-powered centrifuge and a fiberoptic Paralens enabled us to complete and interpret the assay in this remote environment. Of 322 QBC tubes examined for 2-4 min each, results of 295 (92%) concurred with findings on the matched Giemsa-stained thick smear (GTS). The 27 discrepant results included 13 QBC+/GTS- that, upon reexamination, were found to be GTS+. When using the corrected GTS results as the standard, the sensitivity and specificity of the QBC were 94% and 96%, respectively. Because electricity was available only 3 hr per day, it was decided to decrease the examination for an additional 248 QBC to a maximum of 90 sec per tube. This shortened inspection time resulted in a reduction of sensitivity to 53% but specificity was preserved at 89%. Forty-two of 45 conflicting results, QBC-/GTS+ from cases of light Plasmodium falciparum infections with < 1 trophozoite or gametocyte per field, were resolved by reexamination of the QBC in the laboratory. Tubes held at 4 C could be reexamined, without noticeable loss of fluorescence, for at least 6 wk after collection. Despite some difficulty in the identification of Plasmodium species, it was concluded that the QBC is an easy, sensitive method for the rapid diagnosis of malaria in the field and that it provides the inexperienced microscopist with an additional means for on-site identification of individuals needing treatment. SN - 0022-3395 UR - https://www.unboundmedicine.com/medline/citation/1491313/On_site_diagnosis_of_Plasmodium_falciparum_P__vivax_and_P__malariae_by_using_the_Quantitative_Buffy_Coat_system_ DB - PRIME DP - Unbound Medicine ER -