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Interdigitated Array microelectrode-based electrochemical impedance immunosensor for detection of Escherichia coli O157:H7.
Anal Chem. 2004 Feb 15; 76(4):1107-13.AC

Abstract

A label-free electrochemical impedance immunosensor for rapid detection of Escherichia coli O157:H7 was developed by immobilizing anti-E. coli antibodies onto an indium-tin oxide interdigitated array (IDA) microelectrode. Based on the general electronic equivalent model of an electrochemical cell and the behavior of the IDA microelectrode, an equivalent circuit, consisting of an ohmic resistor of the electrolyte between two electrodes and a double layer capacitor, an electron-transfer resistor, and a Warburg impedance around each electrode, was introduced for interpretation of the impedance components of the IDA microelectrode system. The results showed that the immobilization of antibodies and the binding of E. coli cells to the IDA microelectrode surface increased the electron-transfer resistance, which was directly measured with electrochemical impedance spectroscopy in the presence of [Fe(CN)(6)](3-/4-) as a redox probe. The electron-transfer resistance was correlated with the concentration of E. coli cells in a range from 4.36 x 10(5) to 4.36 x 10(8) cfu/mL with the detection limit of 10(6) cfu/mL.

Authors+Show Affiliations

Department of Biological & Agricultural Engineering, and Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14961745

Citation

Yang, Liju, et al. "Interdigitated Array Microelectrode-based Electrochemical Impedance Immunosensor for Detection of Escherichia Coli O157:H7." Analytical Chemistry, vol. 76, no. 4, 2004, pp. 1107-13.
Yang L, Li Y, Erf GF. Interdigitated Array microelectrode-based electrochemical impedance immunosensor for detection of Escherichia coli O157:H7. Anal Chem. 2004;76(4):1107-13.
Yang, L., Li, Y., & Erf, G. F. (2004). Interdigitated Array microelectrode-based electrochemical impedance immunosensor for detection of Escherichia coli O157:H7. Analytical Chemistry, 76(4), 1107-13.
Yang L, Li Y, Erf GF. Interdigitated Array Microelectrode-based Electrochemical Impedance Immunosensor for Detection of Escherichia Coli O157:H7. Anal Chem. 2004 Feb 15;76(4):1107-13. PubMed PMID: 14961745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interdigitated Array microelectrode-based electrochemical impedance immunosensor for detection of Escherichia coli O157:H7. AU - Yang,Liju, AU - Li,Yanbin, AU - Erf,Gisela F, PY - 2004/2/14/pubmed PY - 2004/8/6/medline PY - 2004/2/14/entrez SP - 1107 EP - 13 JF - Analytical chemistry JO - Anal Chem VL - 76 IS - 4 N2 - A label-free electrochemical impedance immunosensor for rapid detection of Escherichia coli O157:H7 was developed by immobilizing anti-E. coli antibodies onto an indium-tin oxide interdigitated array (IDA) microelectrode. Based on the general electronic equivalent model of an electrochemical cell and the behavior of the IDA microelectrode, an equivalent circuit, consisting of an ohmic resistor of the electrolyte between two electrodes and a double layer capacitor, an electron-transfer resistor, and a Warburg impedance around each electrode, was introduced for interpretation of the impedance components of the IDA microelectrode system. The results showed that the immobilization of antibodies and the binding of E. coli cells to the IDA microelectrode surface increased the electron-transfer resistance, which was directly measured with electrochemical impedance spectroscopy in the presence of [Fe(CN)(6)](3-/4-) as a redox probe. The electron-transfer resistance was correlated with the concentration of E. coli cells in a range from 4.36 x 10(5) to 4.36 x 10(8) cfu/mL with the detection limit of 10(6) cfu/mL. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/14961745/Interdigitated_Array_microelectrode_based_electrochemical_impedance_immunosensor_for_detection_of_Escherichia_coli_O157:H7_ DB - PRIME DP - Unbound Medicine ER -