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Clenbuterol treatment affects myosin heavy chain isoforms and MyoD content similarly in intact and regenerated soleus muscles.
Acta Physiol Scand. 2004 Mar; 180(3):271-80.AP

Abstract

AIMS

Pharmacological treatment with the beta2-adrenoceptor agonist clenbuterol is known to induce a slow-to-fast fibre type and myosin heavy chain (MHC) isoform transition in intact muscle. This study examined the sensitivity of regenerated soleus muscle to 4 weeks of clenbuterol treatment (2 mg kg-1 day-1).

METHODS

Female Wistar rats were divided into two groups: vehicle treated (n = 8) and clenbuterol treated (n = 8). The clenbuterol effects on MHC and MyoD expression were examined in soleus muscles either intact, or previously degenerated by venom of the Notechis scutatus scutatus snake.

RESULTS

Post-treatment body weights and skeletal muscle weights were not affected by clenbuterol treatment. Muscle protein concentration was higher, and body fat lower in clenbuterol-treated rats than in vehicle-treated animals (P < 0.05). Polyacrylamide gel electrophoresis of soleus myofibrillar protein indicated a clenbuterol-induced decrease in the relative percentage of type I MHC with a concomitant increase in type IIa MHC (31%, P < 0.001). No degeneration effect was observed after 28 days of recovery on the MHC isoform content, and regenerated soleus muscles exhibited the same phenotypical profile as intact soleus muscles, whether or not they were treated with clenbuterol. In intact and in regenerated soleus muscles, MyoD protein levels were significantly increased by clenbuterol treatment (90 and 77%, respectively, P < 0.001).

CONCLUSION

These results show that regenerated soleus muscles, comprising a homogeneous population of fibres deriving from satellite cells, have a similar response to clenbuterol as intact muscle arising from at least two discrete populations of myotubes; it is suggested that the activity of signalling pathways involved in the effects of clenbuterol on MHC transitions is not related to the developmental history of myofibres.

Authors+Show Affiliations

Department of Human Factors, Centre de recherches du service de santé des armées, La Tronche Cedex, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14962009

Citation

Bricout, V-A, et al. "Clenbuterol Treatment Affects Myosin Heavy Chain Isoforms and MyoD Content Similarly in Intact and Regenerated Soleus Muscles." Acta Physiologica Scandinavica, vol. 180, no. 3, 2004, pp. 271-80.
Bricout VA, Serrurier BD, Bigard AX. Clenbuterol treatment affects myosin heavy chain isoforms and MyoD content similarly in intact and regenerated soleus muscles. Acta Physiol Scand. 2004;180(3):271-80.
Bricout, V. A., Serrurier, B. D., & Bigard, A. X. (2004). Clenbuterol treatment affects myosin heavy chain isoforms and MyoD content similarly in intact and regenerated soleus muscles. Acta Physiologica Scandinavica, 180(3), 271-80.
Bricout VA, Serrurier BD, Bigard AX. Clenbuterol Treatment Affects Myosin Heavy Chain Isoforms and MyoD Content Similarly in Intact and Regenerated Soleus Muscles. Acta Physiol Scand. 2004;180(3):271-80. PubMed PMID: 14962009.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clenbuterol treatment affects myosin heavy chain isoforms and MyoD content similarly in intact and regenerated soleus muscles. AU - Bricout,V-A, AU - Serrurier,B D, AU - Bigard,A X, PY - 2004/2/14/pubmed PY - 2004/6/29/medline PY - 2004/2/14/entrez SP - 271 EP - 80 JF - Acta physiologica Scandinavica JO - Acta Physiol Scand VL - 180 IS - 3 N2 - AIMS: Pharmacological treatment with the beta2-adrenoceptor agonist clenbuterol is known to induce a slow-to-fast fibre type and myosin heavy chain (MHC) isoform transition in intact muscle. This study examined the sensitivity of regenerated soleus muscle to 4 weeks of clenbuterol treatment (2 mg kg-1 day-1). METHODS: Female Wistar rats were divided into two groups: vehicle treated (n = 8) and clenbuterol treated (n = 8). The clenbuterol effects on MHC and MyoD expression were examined in soleus muscles either intact, or previously degenerated by venom of the Notechis scutatus scutatus snake. RESULTS: Post-treatment body weights and skeletal muscle weights were not affected by clenbuterol treatment. Muscle protein concentration was higher, and body fat lower in clenbuterol-treated rats than in vehicle-treated animals (P < 0.05). Polyacrylamide gel electrophoresis of soleus myofibrillar protein indicated a clenbuterol-induced decrease in the relative percentage of type I MHC with a concomitant increase in type IIa MHC (31%, P < 0.001). No degeneration effect was observed after 28 days of recovery on the MHC isoform content, and regenerated soleus muscles exhibited the same phenotypical profile as intact soleus muscles, whether or not they were treated with clenbuterol. In intact and in regenerated soleus muscles, MyoD protein levels were significantly increased by clenbuterol treatment (90 and 77%, respectively, P < 0.001). CONCLUSION: These results show that regenerated soleus muscles, comprising a homogeneous population of fibres deriving from satellite cells, have a similar response to clenbuterol as intact muscle arising from at least two discrete populations of myotubes; it is suggested that the activity of signalling pathways involved in the effects of clenbuterol on MHC transitions is not related to the developmental history of myofibres. SN - 0001-6772 UR - https://www.unboundmedicine.com/medline/citation/14962009/Clenbuterol_treatment_affects_myosin_heavy_chain_isoforms_and_MyoD_content_similarly_in_intact_and_regenerated_soleus_muscles_ DB - PRIME DP - Unbound Medicine ER -