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Inflammatory effects of coarse and fine particulate matter in relation to chemical and biological constituents.
Toxicol Appl Pharmacol. 2004 Feb 15; 195(1):1-11.TA

Abstract

There is conflicting evidence in the literature as to the predominant mechanism and also the compositional element(s) that drives the pulmonary inflammatory response of ambient particulate matter (PM). We have investigated the inflammogenic potential of coarse (2.5-10 microm) and fine (<2.5 microm) PM from both a rural and an industrial location in Germany, using bronchoalveolar lavage (BAL) of rat lungs 18 h post intratracheal instillation with PM. Irrespective of the sampling location, the coarse fraction of PM(10) but not its fine counterpart caused neutrophilic inflammation in rat lungs, in the absence of any severe pulmonary toxicity as indicated by the lack of an increase in lavage protein and lactate dehydrogenase levels. The rural sample of coarse PM also caused a significant increase in the tumor necrosis factor alpha (TNFalpha) content as well as glutathione depletion in the BAL fluid. The contrasting inflammatory responses of the different samples could not be explained by differences in the concentrations of soluble Fe, Cu, V, Ni, Cr, or Al or by the.OH generating capacities of the PM suspensions. However, the effects of the different PM samples were clearly associated with their endotoxin content, as well as their ability to induce interleukin (IL)-8 and TNFalpha from whole blood in vitro. In conclusion, on an equal mass basis, coarse but not fine PM samples from our sampling campaign induced an inflammatory reaction in the lung in the absence of gross cellular lung damage, following intratracheal instillation. Our data also indicate, in accordance with previous independent in vitro observations, that endotoxin or related contaminants may play a role in these in vivo effects.

Authors+Show Affiliations

Institut für Umweltmedizinische Forschung (IUF) an der Heinrich-Heine University, Düsseldorf, Germany. roel.schins@uni-duesseldorf.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14962500

Citation

Schins, Roel P F., et al. "Inflammatory Effects of Coarse and Fine Particulate Matter in Relation to Chemical and Biological Constituents." Toxicology and Applied Pharmacology, vol. 195, no. 1, 2004, pp. 1-11.
Schins RP, Lightbody JH, Borm PJ, et al. Inflammatory effects of coarse and fine particulate matter in relation to chemical and biological constituents. Toxicol Appl Pharmacol. 2004;195(1):1-11.
Schins, R. P., Lightbody, J. H., Borm, P. J., Shi, T., Donaldson, K., & Stone, V. (2004). Inflammatory effects of coarse and fine particulate matter in relation to chemical and biological constituents. Toxicology and Applied Pharmacology, 195(1), 1-11.
Schins RP, et al. Inflammatory Effects of Coarse and Fine Particulate Matter in Relation to Chemical and Biological Constituents. Toxicol Appl Pharmacol. 2004 Feb 15;195(1):1-11. PubMed PMID: 14962500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inflammatory effects of coarse and fine particulate matter in relation to chemical and biological constituents. AU - Schins,Roel P F, AU - Lightbody,Janet H, AU - Borm,Paul J A, AU - Shi,Tingming, AU - Donaldson,Ken, AU - Stone,Vicki, PY - 2003/05/21/received PY - 2003/10/06/accepted PY - 2004/2/14/pubmed PY - 2004/3/23/medline PY - 2004/2/14/entrez SP - 1 EP - 11 JF - Toxicology and applied pharmacology JO - Toxicol Appl Pharmacol VL - 195 IS - 1 N2 - There is conflicting evidence in the literature as to the predominant mechanism and also the compositional element(s) that drives the pulmonary inflammatory response of ambient particulate matter (PM). We have investigated the inflammogenic potential of coarse (2.5-10 microm) and fine (<2.5 microm) PM from both a rural and an industrial location in Germany, using bronchoalveolar lavage (BAL) of rat lungs 18 h post intratracheal instillation with PM. Irrespective of the sampling location, the coarse fraction of PM(10) but not its fine counterpart caused neutrophilic inflammation in rat lungs, in the absence of any severe pulmonary toxicity as indicated by the lack of an increase in lavage protein and lactate dehydrogenase levels. The rural sample of coarse PM also caused a significant increase in the tumor necrosis factor alpha (TNFalpha) content as well as glutathione depletion in the BAL fluid. The contrasting inflammatory responses of the different samples could not be explained by differences in the concentrations of soluble Fe, Cu, V, Ni, Cr, or Al or by the.OH generating capacities of the PM suspensions. However, the effects of the different PM samples were clearly associated with their endotoxin content, as well as their ability to induce interleukin (IL)-8 and TNFalpha from whole blood in vitro. In conclusion, on an equal mass basis, coarse but not fine PM samples from our sampling campaign induced an inflammatory reaction in the lung in the absence of gross cellular lung damage, following intratracheal instillation. Our data also indicate, in accordance with previous independent in vitro observations, that endotoxin or related contaminants may play a role in these in vivo effects. SN - 0041-008X UR - https://www.unboundmedicine.com/medline/citation/14962500/Inflammatory_effects_of_coarse_and_fine_particulate_matter_in_relation_to_chemical_and_biological_constituents_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041008X03005015 DB - PRIME DP - Unbound Medicine ER -