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[Studies on the polyclonal antibody and the double-antibody enzyme-linked immunosorbent assay against E. coli O157 in food].
Wei Sheng Yan Jiu. 2003 Nov; 32(6):606-9.WS

Abstract

The polyclonal antibody (PcAb) for E. coli O157:H7 was available by intravenous injection with New Zealand rabbit with E. coli O157:H7 ATCC43895 strain. Together with the monoclonal antibody (McAb) 3A5 specific against E. coli O157 and O113:H21, a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was developed with the as the capture antibody and the McAb 3A5 as the detection antibody. The detection limits are 10(3)-10(4) cfu/ml in pure culture of E. coli O157 and without any cross reaction with the non-O157 strains. With 10 h enrichment procedure, E. coli O157:H7 recovered well from inoculated ground poultry meat and pasteurized milk at levels of 0.1 cfu/g(cfu/ml). The results demonstrated that the double-antibody sandwich ELISA is a sensitive and specific method for detecting E. coli O157 in food samples.

Authors+Show Affiliations

Zhao Z
National Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Liu X
No affiliation info available

MeSH

AnimalsAntibodies, BacterialAntibodies, MonoclonalEnzyme-Linked Immunosorbent AssayEscherichia coli InfectionsEscherichia coli O157FemaleFood MicrobiologyMeatMilkPoultry ProductsRabbitsSensitivity and Specificity

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

14963916

Citation

Zhao, Zhijing, and Xiumei Liu. "[Studies On the Polyclonal Antibody and the Double-antibody Enzyme-linked Immunosorbent Assay Against E. Coli O157 in Food]." Wei Sheng Yan Jiu = Journal of Hygiene Research, vol. 32, no. 6, 2003, pp. 606-9.
Zhao Z, Liu X. [Studies on the polyclonal antibody and the double-antibody enzyme-linked immunosorbent assay against E. coli O157 in food]. Wei Sheng Yan Jiu. 2003;32(6):606-9.
Zhao, Z., & Liu, X. (2003). [Studies on the polyclonal antibody and the double-antibody enzyme-linked immunosorbent assay against E. coli O157 in food]. Wei Sheng Yan Jiu = Journal of Hygiene Research, 32(6), 606-9.
Zhao Z, Liu X. [Studies On the Polyclonal Antibody and the Double-antibody Enzyme-linked Immunosorbent Assay Against E. Coli O157 in Food]. Wei Sheng Yan Jiu. 2003;32(6):606-9. PubMed PMID: 14963916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Studies on the polyclonal antibody and the double-antibody enzyme-linked immunosorbent assay against E. coli O157 in food]. AU - Zhao,Zhijing, AU - Liu,Xiumei, PY - 2004/2/18/pubmed PY - 2004/7/23/medline PY - 2004/2/18/entrez SP - 606 EP - 9 JF - Wei sheng yan jiu = Journal of hygiene research JO - Wei Sheng Yan Jiu VL - 32 IS - 6 N2 - The polyclonal antibody (PcAb) for E. coli O157:H7 was available by intravenous injection with New Zealand rabbit with E. coli O157:H7 ATCC43895 strain. Together with the monoclonal antibody (McAb) 3A5 specific against E. coli O157 and O113:H21, a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was developed with the as the capture antibody and the McAb 3A5 as the detection antibody. The detection limits are 10(3)-10(4) cfu/ml in pure culture of E. coli O157 and without any cross reaction with the non-O157 strains. With 10 h enrichment procedure, E. coli O157:H7 recovered well from inoculated ground poultry meat and pasteurized milk at levels of 0.1 cfu/g(cfu/ml). The results demonstrated that the double-antibody sandwich ELISA is a sensitive and specific method for detecting E. coli O157 in food samples. SN - 1000-8020 UR - https://www.unboundmedicine.com/medline/citation/14963916/[Studies_on_the_polyclonal_antibody_and_the_double_antibody_enzyme_linked_immunosorbent_assay_against_E__coli_O157_in_food]_ DB - PRIME DP - Unbound Medicine ER -