[Studies on the polyclonal antibody and the double-antibody enzyme-linked immunosorbent assay against E. coli O157 in food].Wei Sheng Yan Jiu. 2003 Nov; 32(6):606-9.WS
The polyclonal antibody (PcAb) for E. coli O157:H7 was available by intravenous injection with New Zealand rabbit with E. coli O157:H7 ATCC43895 strain. Together with the monoclonal antibody (McAb) 3A5 specific against E. coli O157 and O113:H21, a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was developed with the as the capture antibody and the McAb 3A5 as the detection antibody. The detection limits are 10(3)-10(4) cfu/ml in pure culture of E. coli O157 and without any cross reaction with the non-O157 strains. With 10 h enrichment procedure, E. coli O157:H7 recovered well from inoculated ground poultry meat and pasteurized milk at levels of 0.1 cfu/g(cfu/ml). The results demonstrated that the double-antibody sandwich ELISA is a sensitive and specific method for detecting E. coli O157 in food samples.