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The effect of Botrytis cinerea infection on the antioxidant profile of mitochondria from tomato leaves.
J Exp Bot. 2004 Mar; 55(397):605-12.JE

Abstract

Infection of tomato leaves with the necrotrophic fungus Botrytis cinerea resulted in substantial changes in enzymatic and non-enzymatic components of the ascorbate-glutathione cycle as well as in superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione transferase (GST), and l-galactono-gamma-lactone dehydrogenase (GLDH) activities. In the initial phase of the 5 d experiment CuZn SOD was the most rapidly induced isoform (up to 209% of control), whereas later on its activity increase was not concomitant with the constant total SOD enhancement. Starting from the second day B. cinerea infection diminished the mitochondrial antioxidant capacity by decreasing activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) as well as declining ascorbate and glutathione contents. This was accompanied by dehydroascorbate (DHA) and oxidized glutathione (GSSG) accumulation that resulted in ascorbate and glutathione redox ratios decreases. The strongest redox ratio decline of 29% for ascorbate and of 34% for glutathione was found on the 3rd and 2nd days, respectively. Glutathione reductase (GR) induction (185% of control 2 d after inoculation) was insufficient to overcome the decreased antioxidant potential of glutathione. Changes in the ascorbate pool size were closely related to the activity of l-galactono-gamma-lactone dehydrogenase (GLDH). The activities of two glutathione-dependent enzymes: GSH-Px and GST were increased from day 1 to day 4. These results demonstrated that in B. cinerea-tomato interaction mitochondria could be one of the main targets for infection-induced oxidative stress.

Authors+Show Affiliations

Department of Plant Physiology and Biochemistry, University of Łódź, 90-237 Łódź, Banacha 12/16, Poland. elkuz@biol.uni.lodz.plNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14966215

Citation

Kuzniak, Elzbieta, and Maria Skłodowska. "The Effect of Botrytis Cinerea Infection On the Antioxidant Profile of Mitochondria From Tomato Leaves." Journal of Experimental Botany, vol. 55, no. 397, 2004, pp. 605-12.
Kuzniak E, Skłodowska M. The effect of Botrytis cinerea infection on the antioxidant profile of mitochondria from tomato leaves. J Exp Bot. 2004;55(397):605-12.
Kuzniak, E., & Skłodowska, M. (2004). The effect of Botrytis cinerea infection on the antioxidant profile of mitochondria from tomato leaves. Journal of Experimental Botany, 55(397), 605-12.
Kuzniak E, Skłodowska M. The Effect of Botrytis Cinerea Infection On the Antioxidant Profile of Mitochondria From Tomato Leaves. J Exp Bot. 2004;55(397):605-12. PubMed PMID: 14966215.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of Botrytis cinerea infection on the antioxidant profile of mitochondria from tomato leaves. AU - Kuzniak,Elzbieta, AU - Skłodowska,Maria, Y1 - 2004/02/13/ PY - 2004/2/18/pubmed PY - 2004/7/20/medline PY - 2004/2/18/entrez SP - 605 EP - 12 JF - Journal of experimental botany JO - J Exp Bot VL - 55 IS - 397 N2 - Infection of tomato leaves with the necrotrophic fungus Botrytis cinerea resulted in substantial changes in enzymatic and non-enzymatic components of the ascorbate-glutathione cycle as well as in superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione transferase (GST), and l-galactono-gamma-lactone dehydrogenase (GLDH) activities. In the initial phase of the 5 d experiment CuZn SOD was the most rapidly induced isoform (up to 209% of control), whereas later on its activity increase was not concomitant with the constant total SOD enhancement. Starting from the second day B. cinerea infection diminished the mitochondrial antioxidant capacity by decreasing activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) as well as declining ascorbate and glutathione contents. This was accompanied by dehydroascorbate (DHA) and oxidized glutathione (GSSG) accumulation that resulted in ascorbate and glutathione redox ratios decreases. The strongest redox ratio decline of 29% for ascorbate and of 34% for glutathione was found on the 3rd and 2nd days, respectively. Glutathione reductase (GR) induction (185% of control 2 d after inoculation) was insufficient to overcome the decreased antioxidant potential of glutathione. Changes in the ascorbate pool size were closely related to the activity of l-galactono-gamma-lactone dehydrogenase (GLDH). The activities of two glutathione-dependent enzymes: GSH-Px and GST were increased from day 1 to day 4. These results demonstrated that in B. cinerea-tomato interaction mitochondria could be one of the main targets for infection-induced oxidative stress. SN - 0022-0957 UR - https://www.unboundmedicine.com/medline/citation/14966215/The_effect_of_Botrytis_cinerea_infection_on_the_antioxidant_profile_of_mitochondria_from_tomato_leaves_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erh076 DB - PRIME DP - Unbound Medicine ER -