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Structural assignment of isomeric 2-aminopyridine-derivatized oligosaccharides using MSn spectral matching.
Rapid Commun Mass Spectrom. 2004; 18(4):385-91.RC

Abstract

Two isomeric pairs of 2-aminopyridine (PA)-derivatized fucosylated and non-fucosylated oligosaccharides (complex-type N-glycans of IgG) were analyzed using liquid chromatography/ion trap mass spectrometry (LC/ITMS) with a sonic spray ionization source and by varying the collision-induced dissociation voltage. Reproducibility of MS(n) (n = 2) spectra obtained by LC/ITMS was tested considering both fragment ions (m/z) and intensities. A comparison of their MS(n) spectra and evaluation of similarities (or matching), based on correlation coefficients between MS(n) spectra, was investigated as a possibility for structural assignment of the isomers. It is shown that such MS(n) spectral matching is useful and applicable to the structural assignment of relatively large fucosylated and sialylated PA-oligosaccharides released from IgG based on Bn- and Yn-type fragmentations of the corresponding [M+H+Na](2+) ions.

Authors+Show Affiliations

Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 001-0021, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14966844

Citation

Takegawa, Yasuhiro, et al. "Structural Assignment of Isomeric 2-aminopyridine-derivatized Oligosaccharides Using MSn Spectral Matching." Rapid Communications in Mass Spectrometry : RCM, vol. 18, no. 4, 2004, pp. 385-91.
Takegawa Y, Ito S, Yoshioka S, et al. Structural assignment of isomeric 2-aminopyridine-derivatized oligosaccharides using MSn spectral matching. Rapid Commun Mass Spectrom. 2004;18(4):385-91.
Takegawa, Y., Ito, S., Yoshioka, S., Deguchi, K., Nakagawa, H., Monde, K., & Nishimura, S. (2004). Structural assignment of isomeric 2-aminopyridine-derivatized oligosaccharides using MSn spectral matching. Rapid Communications in Mass Spectrometry : RCM, 18(4), 385-91.
Takegawa Y, et al. Structural Assignment of Isomeric 2-aminopyridine-derivatized Oligosaccharides Using MSn Spectral Matching. Rapid Commun Mass Spectrom. 2004;18(4):385-91. PubMed PMID: 14966844.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural assignment of isomeric 2-aminopyridine-derivatized oligosaccharides using MSn spectral matching. AU - Takegawa,Yasuhiro, AU - Ito,Shinya, AU - Yoshioka,Shinji, AU - Deguchi,Kisaburo, AU - Nakagawa,Hiroaki, AU - Monde,Kenji, AU - Nishimura,Shin-Ichiro, PY - 2004/2/18/pubmed PY - 2004/5/5/medline PY - 2004/2/18/entrez SP - 385 EP - 91 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 18 IS - 4 N2 - Two isomeric pairs of 2-aminopyridine (PA)-derivatized fucosylated and non-fucosylated oligosaccharides (complex-type N-glycans of IgG) were analyzed using liquid chromatography/ion trap mass spectrometry (LC/ITMS) with a sonic spray ionization source and by varying the collision-induced dissociation voltage. Reproducibility of MS(n) (n = 2) spectra obtained by LC/ITMS was tested considering both fragment ions (m/z) and intensities. A comparison of their MS(n) spectra and evaluation of similarities (or matching), based on correlation coefficients between MS(n) spectra, was investigated as a possibility for structural assignment of the isomers. It is shown that such MS(n) spectral matching is useful and applicable to the structural assignment of relatively large fucosylated and sialylated PA-oligosaccharides released from IgG based on Bn- and Yn-type fragmentations of the corresponding [M+H+Na](2+) ions. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/14966844/Structural_assignment_of_isomeric_2_aminopyridine_derivatized_oligosaccharides_using_MSn_spectral_matching_ DB - PRIME DP - Unbound Medicine ER -