TY - JOUR T1 - Structural assignment of isomeric 2-aminopyridine-derivatized oligosaccharides using MSn spectral matching. AU - Takegawa,Yasuhiro, AU - Ito,Shinya, AU - Yoshioka,Shinji, AU - Deguchi,Kisaburo, AU - Nakagawa,Hiroaki, AU - Monde,Kenji, AU - Nishimura,Shin-Ichiro, PY - 2004/2/18/pubmed PY - 2004/5/5/medline PY - 2004/2/18/entrez SP - 385 EP - 91 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 18 IS - 4 N2 - Two isomeric pairs of 2-aminopyridine (PA)-derivatized fucosylated and non-fucosylated oligosaccharides (complex-type N-glycans of IgG) were analyzed using liquid chromatography/ion trap mass spectrometry (LC/ITMS) with a sonic spray ionization source and by varying the collision-induced dissociation voltage. Reproducibility of MS(n) (n = 2) spectra obtained by LC/ITMS was tested considering both fragment ions (m/z) and intensities. A comparison of their MS(n) spectra and evaluation of similarities (or matching), based on correlation coefficients between MS(n) spectra, was investigated as a possibility for structural assignment of the isomers. It is shown that such MS(n) spectral matching is useful and applicable to the structural assignment of relatively large fucosylated and sialylated PA-oligosaccharides released from IgG based on Bn- and Yn-type fragmentations of the corresponding [M+H+Na](2+) ions. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/14966844/Structural_assignment_of_isomeric_2_aminopyridine_derivatized_oligosaccharides_using_MSn_spectral_matching_ DB - PRIME DP - Unbound Medicine ER -