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gyrB as a phylogenetic discriminator for members of the Bacillus anthracis-cereus-thuringiensis group.
J Microbiol Methods 2004; 56(3):383-94JM

Abstract

Bacillus anthracis, the causative agent of the human disease anthrax, Bacillus cereus, a food-borne pathogen capable of causing human illness, and Bacillus thuringiensis, a well-characterized insecticidal toxin producer, all cluster together within a very tight clade (B. cereus group) phylogenetically and are indistinguishable from one another via 16S rDNA sequence analysis. As new pathogens are continually emerging, it is imperative to devise a system capable of rapidly and accurately differentiating closely related, yet phenotypically distinct species. Although the gyrB gene has proven useful in discriminating closely related species, its sequence analysis has not yet been validated by DNA:DNA hybridization, the taxonomically accepted "gold standard". We phylogenetically characterized the gyrB sequences of various species and serotypes encompassed in the "B. cereus group," including lab strains and environmental isolates. Results were compared to those obtained from analyses of phenotypic characteristics, 16S rDNA sequence, DNA:DNA hybridization, and virulence factors. The gyrB gene proved more highly differential than 16S, while, at the same time, as analytical as costly and laborious DNA:DNA hybridization techniques in differentiating species within the B. cereus group.

Authors+Show Affiliations

Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA. mtladuc@jpl.nasa.govNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

14967230

Citation

La Duc, Myron T., et al. "GyrB as a Phylogenetic Discriminator for Members of the Bacillus Anthracis-cereus-thuringiensis Group." Journal of Microbiological Methods, vol. 56, no. 3, 2004, pp. 383-94.
La Duc MT, Satomi M, Agata N, et al. GyrB as a phylogenetic discriminator for members of the Bacillus anthracis-cereus-thuringiensis group. J Microbiol Methods. 2004;56(3):383-94.
La Duc, M. T., Satomi, M., Agata, N., & Venkateswaran, K. (2004). GyrB as a phylogenetic discriminator for members of the Bacillus anthracis-cereus-thuringiensis group. Journal of Microbiological Methods, 56(3), pp. 383-94.
La Duc MT, et al. GyrB as a Phylogenetic Discriminator for Members of the Bacillus Anthracis-cereus-thuringiensis Group. J Microbiol Methods. 2004;56(3):383-94. PubMed PMID: 14967230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - gyrB as a phylogenetic discriminator for members of the Bacillus anthracis-cereus-thuringiensis group. AU - La Duc,Myron T, AU - Satomi,Masataka, AU - Agata,Norio, AU - Venkateswaran,Kasthuri, PY - 2003/11/05/received PY - 2003/11/10/revised PY - 2003/11/10/accepted PY - 2004/2/18/pubmed PY - 2004/4/15/medline PY - 2004/2/18/entrez SP - 383 EP - 94 JF - Journal of microbiological methods JO - J. Microbiol. Methods VL - 56 IS - 3 N2 - Bacillus anthracis, the causative agent of the human disease anthrax, Bacillus cereus, a food-borne pathogen capable of causing human illness, and Bacillus thuringiensis, a well-characterized insecticidal toxin producer, all cluster together within a very tight clade (B. cereus group) phylogenetically and are indistinguishable from one another via 16S rDNA sequence analysis. As new pathogens are continually emerging, it is imperative to devise a system capable of rapidly and accurately differentiating closely related, yet phenotypically distinct species. Although the gyrB gene has proven useful in discriminating closely related species, its sequence analysis has not yet been validated by DNA:DNA hybridization, the taxonomically accepted "gold standard". We phylogenetically characterized the gyrB sequences of various species and serotypes encompassed in the "B. cereus group," including lab strains and environmental isolates. Results were compared to those obtained from analyses of phenotypic characteristics, 16S rDNA sequence, DNA:DNA hybridization, and virulence factors. The gyrB gene proved more highly differential than 16S, while, at the same time, as analytical as costly and laborious DNA:DNA hybridization techniques in differentiating species within the B. cereus group. SN - 0167-7012 UR - https://www.unboundmedicine.com/medline/citation/14967230/gyrB_as_a_phylogenetic_discriminator_for_members_of_the_Bacillus_anthracis_cereus_thuringiensis_group_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167701203003191 DB - PRIME DP - Unbound Medicine ER -