Tags

Type your tag names separated by a space and hit enter

Additional evidence of acute anti-inflammatory effects of cyclosporin A in a murine model of pleurisy.
Transpl Immunol. 2004 Jan; 12(2):151-7.TI

Abstract

INTRODUCTION

It is well known that cyclosporin A (CsA) exhibits important anti-inflammatory effects, besides its immunosuppressive activity. However, the mechanisms by which CsA exerts these effects remain unclear.

OBJECTIVE

This study evaluated whether the acute administration of CsA significantly interfered in leukocyte migration, exudation, myeloperoxidase (MPO) and adenosine-deaminase activities and nitrate/nitrite levels, in a mouse model of pleurisy.

MATERIALS AND METHODS

Pleurisy was induced by carrageenan (1%) treatment and the parameters were analyzed 4 and 48 h after. Groups of animals were previously treated with different doses of CsA and compared with non-treated groups.

RESULTS

CsA (0.1-5 mg/kg, intraperitoneal, 1 h before pleurisy induction) inhibited neutrophil migration (P<0.05), but not the exudation that occurred 4 h after pleurisy induction. At this time, CsA (1 mg/kg, 1 h before) also decreased nitrate/nitrite levels and MPO activity (P<0.01). CsA (2 mg/kg, 0.5 h before) was also effective in decreasing mononuclear influx, exudation and nitrate/nitrite levels 48 h after onset of inflammation.

CONCLUSIONS

These results indicate that the acute administration of CsA is able to reduce the two leukocyte populations that occur both at 4 and 48 h after pleurisy induction, late exudation (48 h), MPO activity (4 h) and nitrate/nitrite levels (4 and 48 h). Taken together, these findings indicate that CsA has acute anti-inflammatory effects in immunocompetent animals.

Authors+Show Affiliations

Department of Clinical Analysis, Center of Health Sciences, Universidade Federal de Santa Catarina, Campus Universitário - Trindade, 88040-970 Florianopolis, SC, Brazil.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14967313

Citation

Monguilhott Dalmarco, Eduardo, et al. "Additional Evidence of Acute Anti-inflammatory Effects of Cyclosporin a in a Murine Model of Pleurisy." Transplant Immunology, vol. 12, no. 2, 2004, pp. 151-7.
Monguilhott Dalmarco E, Fröde TS, Medeiros YS. Additional evidence of acute anti-inflammatory effects of cyclosporin A in a murine model of pleurisy. Transpl Immunol. 2004;12(2):151-7.
Monguilhott Dalmarco, E., Fröde, T. S., & Medeiros, Y. S. (2004). Additional evidence of acute anti-inflammatory effects of cyclosporin A in a murine model of pleurisy. Transplant Immunology, 12(2), 151-7.
Monguilhott Dalmarco E, Fröde TS, Medeiros YS. Additional Evidence of Acute Anti-inflammatory Effects of Cyclosporin a in a Murine Model of Pleurisy. Transpl Immunol. 2004;12(2):151-7. PubMed PMID: 14967313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Additional evidence of acute anti-inflammatory effects of cyclosporin A in a murine model of pleurisy. AU - Monguilhott Dalmarco,Eduardo, AU - Fröde,Tânia Silvia, AU - Medeiros,Yara Santos, PY - 2003/05/20/received PY - 2003/09/08/revised PY - 2003/09/11/accepted PY - 2004/2/18/pubmed PY - 2004/9/15/medline PY - 2004/2/18/entrez SP - 151 EP - 7 JF - Transplant immunology JO - Transpl Immunol VL - 12 IS - 2 N2 - INTRODUCTION: It is well known that cyclosporin A (CsA) exhibits important anti-inflammatory effects, besides its immunosuppressive activity. However, the mechanisms by which CsA exerts these effects remain unclear. OBJECTIVE: This study evaluated whether the acute administration of CsA significantly interfered in leukocyte migration, exudation, myeloperoxidase (MPO) and adenosine-deaminase activities and nitrate/nitrite levels, in a mouse model of pleurisy. MATERIALS AND METHODS: Pleurisy was induced by carrageenan (1%) treatment and the parameters were analyzed 4 and 48 h after. Groups of animals were previously treated with different doses of CsA and compared with non-treated groups. RESULTS: CsA (0.1-5 mg/kg, intraperitoneal, 1 h before pleurisy induction) inhibited neutrophil migration (P<0.05), but not the exudation that occurred 4 h after pleurisy induction. At this time, CsA (1 mg/kg, 1 h before) also decreased nitrate/nitrite levels and MPO activity (P<0.01). CsA (2 mg/kg, 0.5 h before) was also effective in decreasing mononuclear influx, exudation and nitrate/nitrite levels 48 h after onset of inflammation. CONCLUSIONS: These results indicate that the acute administration of CsA is able to reduce the two leukocyte populations that occur both at 4 and 48 h after pleurisy induction, late exudation (48 h), MPO activity (4 h) and nitrate/nitrite levels (4 and 48 h). Taken together, these findings indicate that CsA has acute anti-inflammatory effects in immunocompetent animals. SN - 0966-3274 UR - https://www.unboundmedicine.com/medline/citation/14967313/Additional_evidence_of_acute_anti_inflammatory_effects_of_cyclosporin_A_in_a_murine_model_of_pleurisy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0966-3274(03)00092-3 DB - PRIME DP - Unbound Medicine ER -