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Dietary oxidized cholesterol decreases expression of hepatic microsomal triglyceride transfer protein in rats.
J Nutr Biochem. 2004 Feb; 15(2):103-11.JN

Abstract

The aim of this study was to compare the effects of dietary oxidized cholesterol and pure cholesterol on plasma and very low density lipoprotein (VLDL) lipids and on some parameters of VLDL assembly and secretion in rats fed two different dietary fats. Four groups of male growing Sprague-Dawley rats were fed diets containing pure or oxidized cholesterol (5 g/kg diet) with either coconut oil or salmon oil as dietary fat (100 g/kg diet) for 35 days. Rats fed oxidized cholesterol supplemented diets had significantly lower concentrations of triglycerides and cholesterol in plasma and VLDL than rats fed pure cholesterol supplemented diets irrespective of the type of fat. In addition, rats fed oxidized cholesterol supplemented diets had significantly lower relative concentrations of microsomal triglyceride transfer protein messenger ribonucleic acid (mRNA) than rats fed pure cholesterol supplemented diets. In contrast, hepatic lipid concentrations and the relative concentration of apolipoprotein B mRNA were not influenced by the dietary factors investigated. Parameters of hepatic lipogenesis (relative mRNA concentration of sterol regulatory element binding protein-1c and activity of glucose-6-phosphat dehydrogenase) were significantly reduced by feeding fish oil compared to coconut oil, but were not affected by the type of cholesterol. In conclusion, the data of this study suggest, that dietary oxidized cholesterol affects VLDL assembly and/or secretion by reducing the synthesis of MTP but not by impairing hepatic lipogenesis or synthesis of apolipoprotein B.

Authors+Show Affiliations

Institut für Ernährungswissenschaften, Martin-Luther-Universität Halle-Wittenberg, Halle/Saale, Germany.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

14972350

Citation

Ringseis, Robert, and Klaus Eder. "Dietary Oxidized Cholesterol Decreases Expression of Hepatic Microsomal Triglyceride Transfer Protein in Rats." The Journal of Nutritional Biochemistry, vol. 15, no. 2, 2004, pp. 103-11.
Ringseis R, Eder K. Dietary oxidized cholesterol decreases expression of hepatic microsomal triglyceride transfer protein in rats. J Nutr Biochem. 2004;15(2):103-11.
Ringseis, R., & Eder, K. (2004). Dietary oxidized cholesterol decreases expression of hepatic microsomal triglyceride transfer protein in rats. The Journal of Nutritional Biochemistry, 15(2), 103-11.
Ringseis R, Eder K. Dietary Oxidized Cholesterol Decreases Expression of Hepatic Microsomal Triglyceride Transfer Protein in Rats. J Nutr Biochem. 2004;15(2):103-11. PubMed PMID: 14972350.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary oxidized cholesterol decreases expression of hepatic microsomal triglyceride transfer protein in rats. AU - Ringseis,Robert, AU - Eder,Klaus, PY - 2003/01/21/received PY - 2003/09/20/revised PY - 2003/09/26/accepted PY - 2004/2/20/pubmed PY - 2004/9/17/medline PY - 2004/2/20/entrez SP - 103 EP - 11 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 15 IS - 2 N2 - The aim of this study was to compare the effects of dietary oxidized cholesterol and pure cholesterol on plasma and very low density lipoprotein (VLDL) lipids and on some parameters of VLDL assembly and secretion in rats fed two different dietary fats. Four groups of male growing Sprague-Dawley rats were fed diets containing pure or oxidized cholesterol (5 g/kg diet) with either coconut oil or salmon oil as dietary fat (100 g/kg diet) for 35 days. Rats fed oxidized cholesterol supplemented diets had significantly lower concentrations of triglycerides and cholesterol in plasma and VLDL than rats fed pure cholesterol supplemented diets irrespective of the type of fat. In addition, rats fed oxidized cholesterol supplemented diets had significantly lower relative concentrations of microsomal triglyceride transfer protein messenger ribonucleic acid (mRNA) than rats fed pure cholesterol supplemented diets. In contrast, hepatic lipid concentrations and the relative concentration of apolipoprotein B mRNA were not influenced by the dietary factors investigated. Parameters of hepatic lipogenesis (relative mRNA concentration of sterol regulatory element binding protein-1c and activity of glucose-6-phosphat dehydrogenase) were significantly reduced by feeding fish oil compared to coconut oil, but were not affected by the type of cholesterol. In conclusion, the data of this study suggest, that dietary oxidized cholesterol affects VLDL assembly and/or secretion by reducing the synthesis of MTP but not by impairing hepatic lipogenesis or synthesis of apolipoprotein B. SN - 0955-2863 UR - https://www.unboundmedicine.com/medline/citation/14972350/Dietary_oxidized_cholesterol_decreases_expression_of_hepatic_microsomal_triglyceride_transfer_protein_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955286303001748 DB - PRIME DP - Unbound Medicine ER -