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Multiparameter flow cytometry of fluorescent protein reporters.
Methods Mol Biol. 2004; 263:219-38.MM

Abstract

Reporters based on the green fluorescent protein (GFP) from the jellyfish Aequorea victoria and GFP-like proteins from other marine organisms provide valuable tools to monitor gene transfer and expression noninvasively in living cells. Stable cell lines were generated from the Sp2/0-Ag14 hybridoma that express up to three spectral enhanced versions of GFP, the enhanced cyan fluorescent protein (ECFP), the enhanced green fluorescent protein (EGFP), and the enhanced yellow fluorescent protein (EYFP), and/or a variant of the Discosoma coral red fluorescent protein (DsRed). The panel of lines was used to demonstrate a flow cytometric procedure for simultaneous analysis of all four fluorescent proteins that utilizes dual-laser excitation at 488 nm and 407 nm. Additional schemes for simultaneous detection of two, three or four of these fluorescent proteins are also presented.

Authors+Show Affiliations

Flow Cytometry Facility and Hematopoiesis Department, Holland Laboratory, American Red Cross, Rockville, MD, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14976369

Citation

Hawley, Teresa S., et al. "Multiparameter Flow Cytometry of Fluorescent Protein Reporters." Methods in Molecular Biology (Clifton, N.J.), vol. 263, 2004, pp. 219-38.
Hawley TS, Herbert DJ, Eaker SS, et al. Multiparameter flow cytometry of fluorescent protein reporters. Methods Mol Biol. 2004;263:219-38.
Hawley, T. S., Herbert, D. J., Eaker, S. S., & Hawley, R. G. (2004). Multiparameter flow cytometry of fluorescent protein reporters. Methods in Molecular Biology (Clifton, N.J.), 263, 219-38.
Hawley TS, et al. Multiparameter Flow Cytometry of Fluorescent Protein Reporters. Methods Mol Biol. 2004;263:219-38. PubMed PMID: 14976369.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiparameter flow cytometry of fluorescent protein reporters. AU - Hawley,Teresa S, AU - Herbert,Donald J, AU - Eaker,Shannon S, AU - Hawley,Robert G, PY - 2004/2/21/pubmed PY - 2004/7/3/medline PY - 2004/2/21/entrez SP - 219 EP - 38 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol. Biol. VL - 263 N2 - Reporters based on the green fluorescent protein (GFP) from the jellyfish Aequorea victoria and GFP-like proteins from other marine organisms provide valuable tools to monitor gene transfer and expression noninvasively in living cells. Stable cell lines were generated from the Sp2/0-Ag14 hybridoma that express up to three spectral enhanced versions of GFP, the enhanced cyan fluorescent protein (ECFP), the enhanced green fluorescent protein (EGFP), and the enhanced yellow fluorescent protein (EYFP), and/or a variant of the Discosoma coral red fluorescent protein (DsRed). The panel of lines was used to demonstrate a flow cytometric procedure for simultaneous analysis of all four fluorescent proteins that utilizes dual-laser excitation at 488 nm and 407 nm. Additional schemes for simultaneous detection of two, three or four of these fluorescent proteins are also presented. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/14976369/Multiparameter_flow_cytometry_of_fluorescent_protein_reporters_ L2 - https://dx.doi.org/10.1385/1-59259-773-4:219 DB - PRIME DP - Unbound Medicine ER -