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Zn2+-induced cell death is mediated by the induction of intracellular ROS in ARPE-19 cells.
Curr Eye Res. 2004 Mar; 28(3):195-201.CE

Abstract

PURPOSE

Recent studies have shown that Zn2+ induced cell death in retinal pigment epithelial cells. Here we sought to investigate the mode of Zn2+-induced cell death and the role of reactive oxygen species (ROS) in human retinal pigment epithelial cell line, ARPE-19 cells.

METHODS

Cell viability was measured by MTT assay. Cell death of ARPE-19 cells was measured by annexin V-fluorescein isothiocyanate (FITC) binding assay, TUNEL assay. The formation of intracellular ROS was measured using 2',7'-dichlorofluorescein diacetate (DCFH-DA). The activation of mitogen-activated protein kinase (MAPK) was examined by Western blot analysis.

RESULTS

This study demonstrated that Zn2+ treatment induced both necrosis and apoptosis in ARPE-19 cells. Exposure of ARPE-19 cells to Zn2+ led to the activation of ERK1/2, JNK1/2/3, and p38 MAPKs. The activation of these MAPKs was blocked by treatment with the antioxidant, N-acetylcystein (NAC). More importantly, inhibition of ROS production by NAC completely prevented Zn2+-induced cell death in RPE cells.

CONCLUSIONS

This study suggests that Zn2+ induces both apoptosis and necrosis in ARPE-19 cells and that its cytotoxicity may depend on the induction of intracellular ROS.

Authors+Show Affiliations

Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14977522

Citation

Song, Jeongmin, et al. "Zn2+-induced Cell Death Is Mediated By the Induction of Intracellular ROS in ARPE-19 Cells." Current Eye Research, vol. 28, no. 3, 2004, pp. 195-201.
Song J, Lee SC, Kim SS, et al. Zn2+-induced cell death is mediated by the induction of intracellular ROS in ARPE-19 cells. Curr Eye Res. 2004;28(3):195-201.
Song, J., Lee, S. C., Kim, S. S., Koh, H. J., Kwon, O. W., Kang, J. J., Kim, E. K., Shin, S. H., & Lee, J. H. (2004). Zn2+-induced cell death is mediated by the induction of intracellular ROS in ARPE-19 cells. Current Eye Research, 28(3), 195-201.
Song J, et al. Zn2+-induced Cell Death Is Mediated By the Induction of Intracellular ROS in ARPE-19 Cells. Curr Eye Res. 2004;28(3):195-201. PubMed PMID: 14977522.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Zn2+-induced cell death is mediated by the induction of intracellular ROS in ARPE-19 cells. AU - Song,Jeongmin, AU - Lee,Sung Chul, AU - Kim,Sung Soo, AU - Koh,Hyung J, AU - Kwon,Oh Woong, AU - Kang,Jimmy Jaeyoung, AU - Kim,Eung Kweon, AU - Shin,Seung-Hun, AU - Lee,Joon H, PY - 2004/2/24/pubmed PY - 2004/4/16/medline PY - 2004/2/24/entrez SP - 195 EP - 201 JF - Current eye research JO - Curr Eye Res VL - 28 IS - 3 N2 - PURPOSE: Recent studies have shown that Zn2+ induced cell death in retinal pigment epithelial cells. Here we sought to investigate the mode of Zn2+-induced cell death and the role of reactive oxygen species (ROS) in human retinal pigment epithelial cell line, ARPE-19 cells. METHODS: Cell viability was measured by MTT assay. Cell death of ARPE-19 cells was measured by annexin V-fluorescein isothiocyanate (FITC) binding assay, TUNEL assay. The formation of intracellular ROS was measured using 2',7'-dichlorofluorescein diacetate (DCFH-DA). The activation of mitogen-activated protein kinase (MAPK) was examined by Western blot analysis. RESULTS: This study demonstrated that Zn2+ treatment induced both necrosis and apoptosis in ARPE-19 cells. Exposure of ARPE-19 cells to Zn2+ led to the activation of ERK1/2, JNK1/2/3, and p38 MAPKs. The activation of these MAPKs was blocked by treatment with the antioxidant, N-acetylcystein (NAC). More importantly, inhibition of ROS production by NAC completely prevented Zn2+-induced cell death in RPE cells. CONCLUSIONS: This study suggests that Zn2+ induces both apoptosis and necrosis in ARPE-19 cells and that its cytotoxicity may depend on the induction of intracellular ROS. SN - 0271-3683 UR - https://www.unboundmedicine.com/medline/citation/14977522/Zn2+_induced_cell_death_is_mediated_by_the_induction_of_intracellular_ROS_in_ARPE_19_cells_ L2 - https://www.tandfonline.com/doi/full/10.1076/ceyr.28.3.195.26251 DB - PRIME DP - Unbound Medicine ER -