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Analysis of ephedra-alkaloids using sweeping and cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography methods.
Electrophoresis. 2004 Feb; 25(4-5):670-6.E

Abstract

Two stacking methods of capillary electrophoresis (CE) were developed for the separation of very dilute solutions of ephedra-alkaloids, namely ephedrine, pseudoephedrine, methylephedrine, methylpseudoephedrine, norephedrine, and norpseudoephedrine. A sweeping method which uses a carrier comprised of phosphoric acid, sodium dodecyl sulfate (SDS), diethylamine and acetonitrile permits the detection of the alkaloids down to the 10(-1) microg/mL level, and the cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography (CSEI-Sweep-MEKC) method using phosphoric acid, SDS, and acetronitrile as electrolytes can detect down to the 10(-3) microg/mL level. The former requires the conductance of the sample solution to be adjusted beforehand, and only five peaks were observed, two of which were overlapped. The latter is capable of separating the six alkaloids but has a somewhat poorer reproducibility. Using an optimized injection time, it was found that the more diluted a solution is, the greater the sweeping effect will be. The CSEI-Sweep-MEKC method with a 600 s injection time and a 10(-1) microg/mL solution concentration provides an amplification effect of approximately 10(4). The method is suitable for analyses of dilute herb drug extracts and mouse sera. The effect of buffers on the separation and validation of the methods in this study are also discussed.

Authors+Show Affiliations

Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14981695

Citation

Chiang, Hui-Yu, and Shuenn-Jyi Sheu. "Analysis of Ephedra-alkaloids Using Sweeping and Cation-selective Exhaustive Injection and Sweeping Micellar Electrokinetic Chromatography Methods." Electrophoresis, vol. 25, no. 4-5, 2004, pp. 670-6.
Chiang HY, Sheu SJ. Analysis of ephedra-alkaloids using sweeping and cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography methods. Electrophoresis. 2004;25(4-5):670-6.
Chiang, H. Y., & Sheu, S. J. (2004). Analysis of ephedra-alkaloids using sweeping and cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography methods. Electrophoresis, 25(4-5), 670-6.
Chiang HY, Sheu SJ. Analysis of Ephedra-alkaloids Using Sweeping and Cation-selective Exhaustive Injection and Sweeping Micellar Electrokinetic Chromatography Methods. Electrophoresis. 2004;25(4-5):670-6. PubMed PMID: 14981695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of ephedra-alkaloids using sweeping and cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography methods. AU - Chiang,Hui-Yu, AU - Sheu,Shuenn-Jyi, PY - 2004/2/26/pubmed PY - 2004/10/16/medline PY - 2004/2/26/entrez SP - 670 EP - 6 JF - Electrophoresis JO - Electrophoresis VL - 25 IS - 4-5 N2 - Two stacking methods of capillary electrophoresis (CE) were developed for the separation of very dilute solutions of ephedra-alkaloids, namely ephedrine, pseudoephedrine, methylephedrine, methylpseudoephedrine, norephedrine, and norpseudoephedrine. A sweeping method which uses a carrier comprised of phosphoric acid, sodium dodecyl sulfate (SDS), diethylamine and acetonitrile permits the detection of the alkaloids down to the 10(-1) microg/mL level, and the cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography (CSEI-Sweep-MEKC) method using phosphoric acid, SDS, and acetronitrile as electrolytes can detect down to the 10(-3) microg/mL level. The former requires the conductance of the sample solution to be adjusted beforehand, and only five peaks were observed, two of which were overlapped. The latter is capable of separating the six alkaloids but has a somewhat poorer reproducibility. Using an optimized injection time, it was found that the more diluted a solution is, the greater the sweeping effect will be. The CSEI-Sweep-MEKC method with a 600 s injection time and a 10(-1) microg/mL solution concentration provides an amplification effect of approximately 10(4). The method is suitable for analyses of dilute herb drug extracts and mouse sera. The effect of buffers on the separation and validation of the methods in this study are also discussed. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/14981695/Analysis_of_ephedra_alkaloids_using_sweeping_and_cation_selective_exhaustive_injection_and_sweeping_micellar_electrokinetic_chromatography_methods_ L2 - https://doi.org/10.1002/elps.200305729 DB - PRIME DP - Unbound Medicine ER -