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Antisense KGFR oligonucleotide inhibition of KGF-induced motility in breast cancer cells.
Anticancer Res. 2003 Nov-Dec; 23(6C):4913-9.AR

Abstract

BACKGROUND

The metastasis of breast cancer is known to be directly associated with the motility of breast cancer cells. We have previously shown that keratinocyte growth factor (KGF) enhances the motility of estrogen receptor (ER)-positive breast cancer cells and that this motility response is associated with cellular levels of the KGF receptor (KGFR). Further, we observed that KGF treatment enhanced KGFR gene expression in MCF-7 cells. The objective of the present study was to examine the influence of antisense KGFR oligonucleotide treatment on the KGF-induced motility response in breast cancer cells.

MATERIALS AND METHODS

Both time-lapse video microscopy (TLVM) and culture wounding experiments were used to quantify cell motility. KGFR antisense effects on the expression of KGFR were determined by Western blotting, real time PCR and immunocytochemistry.

RESULTS

Antisense KGFR treatment significantly reduced both KGFR mRNA and protein expression. In addition, the antisense KGFR abolished the KGF-mediated cell motility response as early as 2 h following KGF treatment as observed by TLVM and lasting for up 48 h as observed by culture wounding. CONCLUSIOS: The results of this study indicate that KGFR activation and intracellular signaling mediates the KGF motility effect and suggests that KGFR may be an important new therapeutic target for the treatment or prevention of metastatic progression in breast cancer.

Authors+Show Affiliations

Departments of Pharmaceutical Sciences, Pathology and Surgery, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14981945

Citation

Zang, Xiao-Ping, et al. "Antisense KGFR Oligonucleotide Inhibition of KGF-induced Motility in Breast Cancer Cells." Anticancer Research, vol. 23, no. 6C, 2003, pp. 4913-9.
Zang XP, Lerner MR, Dunn ST, et al. Antisense KGFR oligonucleotide inhibition of KGF-induced motility in breast cancer cells. Anticancer Res. 2003;23(6C):4913-9.
Zang, X. P., Lerner, M. R., Dunn, S. T., Brackett, D. J., & Pento, J. T. (2003). Antisense KGFR oligonucleotide inhibition of KGF-induced motility in breast cancer cells. Anticancer Research, 23(6C), 4913-9.
Zang XP, et al. Antisense KGFR Oligonucleotide Inhibition of KGF-induced Motility in Breast Cancer Cells. Anticancer Res. 2003 Nov-Dec;23(6C):4913-9. PubMed PMID: 14981945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antisense KGFR oligonucleotide inhibition of KGF-induced motility in breast cancer cells. AU - Zang,Xiao-Ping, AU - Lerner,Megan R, AU - Dunn,S Terence, AU - Brackett,Daniel J, AU - Pento,J Thomas, PY - 2004/2/26/pubmed PY - 2004/4/2/medline PY - 2004/2/26/entrez SP - 4913 EP - 9 JF - Anticancer research JO - Anticancer Res VL - 23 IS - 6C N2 - BACKGROUND: The metastasis of breast cancer is known to be directly associated with the motility of breast cancer cells. We have previously shown that keratinocyte growth factor (KGF) enhances the motility of estrogen receptor (ER)-positive breast cancer cells and that this motility response is associated with cellular levels of the KGF receptor (KGFR). Further, we observed that KGF treatment enhanced KGFR gene expression in MCF-7 cells. The objective of the present study was to examine the influence of antisense KGFR oligonucleotide treatment on the KGF-induced motility response in breast cancer cells. MATERIALS AND METHODS: Both time-lapse video microscopy (TLVM) and culture wounding experiments were used to quantify cell motility. KGFR antisense effects on the expression of KGFR were determined by Western blotting, real time PCR and immunocytochemistry. RESULTS: Antisense KGFR treatment significantly reduced both KGFR mRNA and protein expression. In addition, the antisense KGFR abolished the KGF-mediated cell motility response as early as 2 h following KGF treatment as observed by TLVM and lasting for up 48 h as observed by culture wounding. CONCLUSIOS: The results of this study indicate that KGFR activation and intracellular signaling mediates the KGF motility effect and suggests that KGFR may be an important new therapeutic target for the treatment or prevention of metastatic progression in breast cancer. SN - 0250-7005 UR - https://www.unboundmedicine.com/medline/citation/14981945/Antisense_KGFR_oligonucleotide_inhibition_of_KGF_induced_motility_in_breast_cancer_cells_ L2 - https://www.diseaseinfosearch.org/result/960 DB - PRIME DP - Unbound Medicine ER -