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Characterization of severe acute respiratory syndrome coronavirus genomes in Taiwan: molecular epidemiology and genome evolution.
Proc Natl Acad Sci U S A. 2004 Feb 24; 101(8):2542-7.PN

Abstract

Since early March 2003, the severe acute respiratory syndrome (SARS) coronavirus (CoV) infection has claimed 346 cases and 37 deaths in Taiwan. The epidemic occurred in two stages. The first stage caused limited familial or hospital infections and lasted from early March to mid-April. All cases had clear contact histories, primarily from Guangdong or Hong Kong. The second stage resulted in a large outbreak in a municipal hospital, and quickly spread to northern and southern Taiwan from late April to mid-June. During this stage, there were some sporadic cases with untraceable contact histories. To investigate the origin and transmission route of SARS-CoV in Taiwan's epidemic, we conducted a systematic viral lineage study by sequencing the entire viral genome from ten SARS patients. SARS-CoV viruses isolated from Taiwan were found closely related to those from Guangdong and Hong Kong. In addition, all cases from the second stage belonged to the same lineage after the municipal hospital outbreak, including the patients without an apparent contact history. Analyses of these full-length sequences showed a positive selection occurring during SARS-CoV virus evolution. The mismatch distribution indicated that SARS viral genomes did not reach equilibrium and suggested a recent introduction of the viruses into human populations. The estimated genome mutation rate was approximately 0.1 per genome, demonstrating possibly one of the lowest rates among known RNA viruses.

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Authors+Show Affiliations

Yeh SH
Division of Molecular and Genomic Medicine, National Health Research Institutes, Taipei 115, Taiwan.
Wang HY
No affiliation info available
Tsai CY
No affiliation info available
Kao CL
No affiliation info available
Yang JY
No affiliation info available
Liu HW
No affiliation info available
Su IJ
No affiliation info available
Tsai SF
No affiliation info available
Chen DS
No affiliation info available
Chen PJ
No affiliation info available
National Taiwan University SARS Research Team
No affiliation info available

MeSH

Base PairingBase SequenceDNA, ViralEvolution, MolecularGenome, ViralHumansMolecular Sequence DataMutationPhylogenyPolymerase Chain ReactionRNA, ViralSevere acute respiratory syndrome-related coronavirusSevere Acute Respiratory SyndromeTaiwan

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14983045

Citation

Yeh, Shiou-Hwei, et al. "Characterization of Severe Acute Respiratory Syndrome Coronavirus Genomes in Taiwan: Molecular Epidemiology and Genome Evolution." Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 8, 2004, pp. 2542-7.
Yeh SH, Wang HY, Tsai CY, et al. Characterization of severe acute respiratory syndrome coronavirus genomes in Taiwan: molecular epidemiology and genome evolution. Proc Natl Acad Sci U S A. 2004;101(8):2542-7.
Yeh, S. H., Wang, H. Y., Tsai, C. Y., Kao, C. L., Yang, J. Y., Liu, H. W., Su, I. J., Tsai, S. F., Chen, D. S., & Chen, P. J. (2004). Characterization of severe acute respiratory syndrome coronavirus genomes in Taiwan: molecular epidemiology and genome evolution. Proceedings of the National Academy of Sciences of the United States of America, 101(8), 2542-7.
Yeh SH, et al. Characterization of Severe Acute Respiratory Syndrome Coronavirus Genomes in Taiwan: Molecular Epidemiology and Genome Evolution. Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2542-7. PubMed PMID: 14983045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of severe acute respiratory syndrome coronavirus genomes in Taiwan: molecular epidemiology and genome evolution. AU - Yeh,Shiou-Hwei, AU - Wang,Hurng-Yi, AU - Tsai,Ching-Yi, AU - Kao,Chuan-Liang, AU - Yang,Jyh-Yuan, AU - Liu,Hwan-Wun, AU - Su,Ih-Jen, AU - Tsai,Shih-Feng, AU - Chen,Ding-Shinn, AU - Chen,Pei-Jer, AU - ,, PY - 2004/2/26/pubmed PY - 2004/4/10/medline PY - 2004/2/26/entrez SP - 2542 EP - 7 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 101 IS - 8 N2 - Since early March 2003, the severe acute respiratory syndrome (SARS) coronavirus (CoV) infection has claimed 346 cases and 37 deaths in Taiwan. The epidemic occurred in two stages. The first stage caused limited familial or hospital infections and lasted from early March to mid-April. All cases had clear contact histories, primarily from Guangdong or Hong Kong. The second stage resulted in a large outbreak in a municipal hospital, and quickly spread to northern and southern Taiwan from late April to mid-June. During this stage, there were some sporadic cases with untraceable contact histories. To investigate the origin and transmission route of SARS-CoV in Taiwan's epidemic, we conducted a systematic viral lineage study by sequencing the entire viral genome from ten SARS patients. SARS-CoV viruses isolated from Taiwan were found closely related to those from Guangdong and Hong Kong. In addition, all cases from the second stage belonged to the same lineage after the municipal hospital outbreak, including the patients without an apparent contact history. Analyses of these full-length sequences showed a positive selection occurring during SARS-CoV virus evolution. The mismatch distribution indicated that SARS viral genomes did not reach equilibrium and suggested a recent introduction of the viruses into human populations. The estimated genome mutation rate was approximately 0.1 per genome, demonstrating possibly one of the lowest rates among known RNA viruses. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/14983045/Characterization_of_severe_acute_respiratory_syndrome_coronavirus_genomes_in_Taiwan:_molecular_epidemiology_and_genome_evolution_ DB - PRIME DP - Unbound Medicine ER -